Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   MWH27_RS01965 Genome accession   NZ_JALJVV010000001
Coordinates   368313..368762 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain A-5     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 363313..373762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWH27_RS01925 (MWH27_01925) - 363638..363835 (+) 198 WP_121509395.1 hypothetical protein -
  MWH27_RS01930 (MWH27_01930) yckC 363855..364310 (+) 456 WP_014478793.1 RDD family protein -
  MWH27_RS01935 (MWH27_01935) yckD 364392..364724 (+) 333 WP_014478794.1 YckD family protein -
  MWH27_RS01940 (MWH27_01940) - 364852..365274 (+) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  MWH27_RS01945 (MWH27_01945) - 365262..365582 (+) 321 WP_014478796.1 DUF3147 family protein -
  MWH27_RS01950 (MWH27_01950) - 365601..366345 (+) 745 Protein_340 AAA family ATPase -
  MWH27_RS01955 (MWH27_01955) bglC 366418..367851 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  MWH27_RS01960 (MWH27_01960) nin/comJ 367888..368286 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  MWH27_RS01965 (MWH27_01965) nucA/comI 368313..368762 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  MWH27_RS01970 (MWH27_01970) tlpC 368930..370651 (-) 1722 Protein_344 methyl-accepting chemotaxis protein TlpC -
  MWH27_RS01975 (MWH27_01975) hxlB 370762..371319 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  MWH27_RS01980 (MWH27_01980) hxlA 371325..371957 (-) 633 WP_014478804.1 3-hexulose-6-phosphate synthase -
  MWH27_RS01985 (MWH27_01985) hxlR 372186..372548 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence


Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=1112983 MWH27_RS01965 WP_014475770.1 368313..368762(-) (nucA/comI) [Bacillus subtilis strain A-5]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1112983 MWH27_RS01965 WP_014475770.1 368313..368762(-) (nucA/comI) [Bacillus subtilis strain A-5]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987


Multiple sequence alignment