Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K2J19_RS13820 Genome accession   NZ_JAIEUD010000001
Coordinates   2915638..2917071 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain ECC47     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2910638..2922071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2J19_RS13790 (K2J19_13790) elbB 2912170..2912823 (+) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -
  K2J19_RS13795 (K2J19_13795) mtgA 2912820..2913545 (+) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  K2J19_RS13800 (K2J19_13800) npr 2913585..2913857 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  K2J19_RS13805 (K2J19_13805) rapZ 2913854..2914708 (-) 855 WP_003861870.1 RNase adapter RapZ -
  K2J19_RS13810 (K2J19_13810) ptsN 2914754..2915245 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K2J19_RS13815 (K2J19_13815) hpf 2915328..2915615 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  K2J19_RS13820 (K2J19_13820) rpoN/rpoN1 2915638..2917071 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  K2J19_RS13825 (K2J19_13825) lptB 2917119..2917844 (-) 726 WP_023304493.1 LPS export ABC transporter ATP-binding protein -
  K2J19_RS13830 (K2J19_13830) lptA 2917851..2918405 (-) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  K2J19_RS13835 (K2J19_13835) lptC 2918374..2918949 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  K2J19_RS13840 (K2J19_13840) kdsC 2918946..2919512 (-) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  K2J19_RS13845 (K2J19_13845) kdsD 2919527..2920513 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  K2J19_RS13850 (K2J19_13850) - 2920527..2921504 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=1112873 K2J19_RS13820 WP_003861864.1 2915638..2917071(-) (rpoN/rpoN1) [Enterobacter hormaechei strain ECC47]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1112873 K2J19_RS13820 WP_003861864.1 2915638..2917071(-) (rpoN/rpoN1) [Enterobacter hormaechei strain ECC47]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGACGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACTCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCAGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment