Detailed information NTG1112195

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: I35_RS14395
• Genome accession: NZ_HG938370
• Coordinates: 3106503..3107663 (+)
• Length: 386 a.a.
• NCBI ID: WP_006483736.1
• Uniprot ID: B4EAQ6
• Organism: Burkholderia cenocepacia H111
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3101503..3112663

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I35_RS14365 (I35_2870)	-	3102149..3102754 (+)	606	WP_006483734.1	50S ribosomal protein L25/general stress protein Ctc	-
I35_RS14370 (I35_2871)	pth	3102875..3103474 (+)	600	WP_006499019.1	aminoacyl-tRNA hydrolase	-
I35_RS14375 (I35_2872)	hisC	3103591..3104658 (+)	1068	WP_006499020.1	histidinol-phosphate transaminase	-
I35_RS14380 (I35_2873)	-	3104737..3105003 (-)	267	WP_043206196.1	YfhL family 4Fe-4S dicluster ferredoxin	-
I35_RS14385 (I35_2874)	coaD	3105063..3105560 (-)	498	WP_006477773.1	pantetheine-phosphate adenylyltransferase	-
I35_RS14390 (I35_2875)	rsmD	3105672..3106286 (-)	615	WP_006483748.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
I35_RS14395 (I35_2876)	pilA	3106503..3107663 (+)	1161	WP_006483736.1	signal recognition particle-docking protein FtsY	Machinery gene
I35_RS14400 (I35_2877)	maiA	3107747..3108391 (-)	645	WP_006499022.1	maleylacetoacetate isomerase	-
I35_RS14405 (I35_2879)	ybiB	3108571..3109545 (+)	975	WP_006483732.1	DNA-binding protein YbiB	-
I35_RS14410 (I35_2881)	leuA	3109914..3111560 (+)	1647	WP_006499025.1	2-isopropylmalate synthase	-
I35_RS14415 (I35_2882)	-	3111705..3112088 (-)	384	WP_006483747.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 40953.90 Da
• Isoelectric Point: 4.7940

>NTDB_id=1112195 I35_RS14395 WP_006483736.1 3106503..3107663(+) (pilA) [Burkholderia cenocepacia H111]
MFSFFKRFKKTQEPDPAESQSADAQQADEASDADAPVLDAPPAASVPPAPAQPAAPAVVMTVTPTNDGRDEVVETVEIVP
PPLQDASAKKSWLARLKTGLAKTGSSITGVFVNTKIDEDLYEELETALLMSDAGVDATEYLLGALREKVRAGRLTDPQQV
KAALHDLLVELLTPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERN
NVTVVQQESGDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVIAKAHDGAPHEVLLVIDANTGQNAL
TQVKAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFSAVEFADALLG

Nucleotide

• Length: 1161 bp

>NTDB_id=1112195 I35_RS14395 WP_006483736.1 3106503..3107663(+) (pilA) [Burkholderia cenocepacia H111]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGATCCGGCGGAATCGCAATCGGCCGACGCGCAGCAAGC
GGACGAAGCGTCCGACGCCGACGCGCCCGTGCTCGACGCGCCGCCCGCGGCCAGCGTGCCGCCGGCGCCCGCGCAACCGG
CTGCGCCGGCCGTCGTGATGACCGTCACGCCGACCAACGACGGCCGCGACGAAGTCGTCGAGACGGTCGAGATCGTGCCG
CCGCCGCTGCAGGACGCGTCCGCGAAGAAGTCGTGGCTCGCGCGCCTGAAGACGGGGCTCGCGAAGACGGGCTCGAGCAT
CACCGGCGTCTTCGTCAACACGAAGATCGACGAGGATCTGTACGAGGAGCTCGAAACCGCGCTGCTGATGTCCGACGCGG
GCGTCGACGCGACCGAATACCTGCTCGGCGCGCTGCGCGAAAAGGTGCGCGCGGGCCGGCTCACCGATCCGCAGCAGGTG
AAGGCTGCACTGCACGACCTGCTCGTCGAGCTGCTGACGCCGCTCGAGAAATCGCTGATGCTCGGCCGCGCGCAGCCGCT
CGTGATGATGATCACCGGCGTGAACGGCGCGGGCAAGACGACCAGCATCGGCAAGCTCGCGAAGCACCTGCAGAGCTTCG
ACCAGTCGGTGCTGCTGGCTGCCGGCGACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCGGTCTGGGGCGAGCGCAAC
AACGTGACGGTCGTGCAGCAGGAGAGCGGCGATCCGGCCGCGGTGATCTTCGACGCGGTCAGCGCCGCGCGCGCGCGCAA
GATCGACGTGATGATGGCCGACACGGCCGGCCGCCTGCCGACGCAGCTGCATCTGATGGAGGAGCTGAAGAAGGTGAAGC
GCGTGATCGCGAAGGCGCACGACGGCGCGCCGCACGAAGTGCTGCTGGTGATCGACGCGAACACCGGCCAGAACGCGCTC
ACGCAGGTGAAGGCCTTCGACGACGCGCTGGGCCTGACCGGCCTGATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGG
GATTCTCGCCGCGATCGCGCGGCAACGGCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGC
CGTTCAGCGCGGTGGAATTCGCGGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (187-386)

• SRP54-type protein, helical bundle domain (SRP54_N) (104-166)

3D structure

• AlphaFold DB: B4EAQ6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	53.606	100	0.578