Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I35_RS14300 Genome accession   NZ_HG938370
Coordinates   3089698..3091203 (+) Length   501 a.a.
NCBI ID   WP_006499010.1    Uniprot ID   -
Organism   Burkholderia cenocepacia H111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3084698..3096203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I35_RS14275 (I35_2853) kdsD 3085929..3086912 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  I35_RS14280 (I35_2854) - 3086912..3087448 (+) 537 WP_006483148.1 KdsC family phosphatase -
  I35_RS14285 (I35_2855) lptC 3087452..3088066 (+) 615 WP_006483145.1 LPS export ABC transporter periplasmic protein LptC -
  I35_RS14290 (I35_2856) lptA 3088087..3088752 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  I35_RS14295 (I35_2857) lptB 3088749..3089525 (+) 777 WP_006483143.1 LPS export ABC transporter ATP-binding protein -
  I35_RS14300 (I35_2858) rpoN/rpoN1 3089698..3091203 (+) 1506 WP_006499010.1 RNA polymerase factor sigma-54 Machinery gene
  I35_RS14305 (I35_2859) hpf 3091362..3091721 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  I35_RS14310 (I35_2860) - 3091917..3092429 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  I35_RS14315 (I35_2861) hprK 3092559..3093527 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  I35_RS14320 (I35_2862) rapZ 3093602..3094510 (+) 909 WP_006483144.1 RNase adapter RapZ -
  I35_RS14325 (I35_2863) - 3094537..3095172 (+) 636 WP_006499012.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54734.88 Da        Isoelectric Point: 4.9301

>NTDB_id=1112194 I35_RS14300 WP_006499010.1 3089698..3091203(+) (rpoN/rpoN1) [Burkholderia cenocepacia H111]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNGSSPSSDRAERDEPAGADEYNGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLAAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1112194 I35_RS14300 WP_006499010.1 3089698..3091203(+) (rpoN/rpoN1) [Burkholderia cenocepacia H111]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCGCCGCG
ACCAACGGCAGCAGCCCGTCCAGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCCGACGAATACAACGGCTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTCGCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCACTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAAGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment