Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BN940_RS16575 Genome accession   NZ_HG916765
Coordinates   3711985..3713283 (-) Length   432 a.a.
NCBI ID   WP_043684826.1    Uniprot ID   W8X5R7
Organism   Castellaniella defragrans 65Phen     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3706985..3718283
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN940_RS16535 (BN940_17106) - 3707004..3707291 (+) 288 WP_052355973.1 toxin-antitoxin system TumE family protein -
  BN940_RS16540 (BN940_17111) - 3707288..3707659 (+) 372 WP_043684814.1 hypothetical protein -
  BN940_RS16545 (BN940_17116) - 3707667..3708173 (-) 507 WP_043684816.1 CinA family protein -
  BN940_RS16550 (BN940_17121) - 3708170..3708727 (-) 558 WP_084330745.1 phosphatidylglycerophosphatase A -
  BN940_RS16555 (BN940_17126) thiL 3708724..3709707 (-) 984 WP_043684819.1 thiamine-phosphate kinase -
  BN940_RS16560 (BN940_17131) nusB 3709727..3710215 (-) 489 WP_043684822.1 transcription antitermination factor NusB -
  BN940_RS16565 (BN940_17136) ribH 3710208..3710741 (-) 534 WP_043684824.1 6,7-dimethyl-8-ribityllumazine synthase -
  BN940_RS16570 (BN940_17141) ribBA 3710758..3711936 (-) 1179 WP_052355974.1 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II -
  BN940_RS16575 (BN940_17146) pilA 3711985..3713283 (-) 1299 WP_043684826.1 signal recognition particle-docking protein FtsY Machinery gene
  BN940_RS16580 (BN940_17151) - 3713341..3713937 (+) 597 WP_043684829.1 RsmD family RNA methyltransferase -
  BN940_RS16585 (BN940_17156) coaD 3714013..3714525 (+) 513 WP_043684832.1 pantetheine-phosphate adenylyltransferase -
  BN940_RS16590 (BN940_17161) - 3714529..3714918 (-) 390 WP_043684835.1 MOSC N-terminal beta barrel domain-containing protein -
  BN940_RS16595 (BN940_17166) - 3714942..3717533 (-) 2592 WP_084330746.1 AsmA family protein -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 44572.65 Da        Isoelectric Point: 4.8598

>NTDB_id=1112102 BN940_RS16575 WP_043684826.1 3711985..3713283(-) (pilA) [Castellaniella defragrans 65Phen]
MFSFFKRKKAPAPQEAAPGRDEALGAAASPDAPEVAPVAPTELPDTWPADAGGLPETGADAPAHEAPAAAAEPVSEFVSG
AGPAPAAAPVQPPDAWSDRPDETADPLVRLDRAASAEAAVPASSGSWLGRLRRGLSRTGQSLGGLFTGVKVDEALFEELE
TALIMADAGVEATEKLLEQLRARVRRQRIERPEAVRAALRDILAEHLKVLEKPFVVGEAAPMVVMIAGVNGAGKTTSIGK
LAHALRERQASVLLAAGDTFRAAAREQLVAWGARNGVTVIAQEGGDPAAVAFDAVNAGRARSADVVLVDTAGRLPTQLHL
MEELKKIKRVIGKADPAAPHEILLVVDGNTGQNALAQIRAFDAALGLTGLVVTKLDGTAKGGTLAAVAAGSQGVRPVPVY
WIGVGEGMQDLQPFVAAEFADALLGGPETDRA

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1112102 BN940_RS16575 WP_043684826.1 3711985..3713283(-) (pilA) [Castellaniella defragrans 65Phen]
ATGTTCAGTTTCTTTAAACGCAAGAAGGCGCCCGCGCCGCAGGAAGCGGCGCCCGGGCGGGACGAAGCCCTCGGCGCCGC
CGCATCCCCGGACGCGCCGGAAGTGGCGCCGGTCGCGCCGACCGAACTGCCCGACACCTGGCCGGCGGATGCCGGCGGGC
TGCCCGAAACCGGCGCCGATGCGCCCGCGCACGAGGCGCCGGCCGCGGCGGCCGAGCCCGTGTCCGAGTTCGTTTCGGGC
GCCGGGCCGGCGCCCGCCGCGGCGCCCGTCCAGCCCCCCGACGCCTGGTCGGACCGGCCGGACGAGACCGCCGATCCCCT
GGTCCGGCTGGACCGCGCGGCCAGCGCCGAGGCGGCCGTGCCCGCGTCCTCGGGCTCCTGGCTGGGCCGGCTGCGGCGCG
GCCTGTCGCGCACCGGCCAGAGCCTGGGCGGCCTGTTCACCGGGGTCAAGGTCGATGAGGCCCTGTTCGAGGAACTGGAA
ACCGCGCTCATCATGGCCGACGCCGGCGTCGAGGCCACGGAAAAGCTGCTGGAGCAGCTGCGCGCGCGCGTGCGGCGCCA
GCGCATCGAGCGGCCCGAGGCCGTGCGCGCCGCGCTGCGCGACATCCTGGCCGAGCACCTGAAGGTCCTGGAAAAGCCCT
TCGTCGTGGGCGAGGCCGCGCCCATGGTGGTCATGATCGCGGGGGTCAATGGCGCGGGCAAGACCACCTCCATCGGCAAG
CTCGCCCATGCCCTGCGGGAACGCCAGGCCTCGGTGCTGCTGGCGGCCGGCGACACCTTCCGCGCGGCGGCGCGCGAGCA
GCTCGTGGCCTGGGGCGCGCGCAACGGCGTCACCGTCATCGCCCAGGAAGGCGGCGATCCGGCGGCCGTGGCCTTCGACG
CGGTCAACGCCGGGCGCGCGCGTTCGGCGGACGTGGTGCTGGTCGATACCGCCGGCCGGCTGCCGACCCAGCTGCACCTG
ATGGAAGAACTGAAGAAGATCAAGCGCGTCATCGGCAAGGCCGATCCGGCGGCGCCCCACGAGATCCTGCTGGTGGTGGA
TGGCAACACCGGCCAGAACGCGCTCGCGCAGATCCGCGCCTTCGACGCCGCGCTGGGCCTGACCGGCCTGGTCGTCACCA
AGCTCGACGGCACGGCCAAGGGCGGGACGCTGGCGGCGGTGGCGGCGGGCAGCCAGGGCGTGCGGCCGGTGCCCGTGTAC
TGGATCGGCGTGGGCGAGGGCATGCAGGACCTGCAGCCCTTCGTCGCGGCGGAGTTCGCCGACGCGCTGCTGGGCGGACC
CGAGACCGACAGGGCCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(221-425)

SRP54-type protein, helical bundle domain (SRP54_N)

(140-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8X5R7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50

100

0.5

Multiple sequence alignment