Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   IR04_RS18525 Genome accession   NZ_CP009042
Coordinates   2929968..2932172 (-) Length   734 a.a.
NCBI ID   WP_001247858.1    Uniprot ID   Q9KNQ3
Organism   Vibrio cholerae O1 biovar El Tor strain FJ147     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2924968..2937172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IR04_RS18505 (IR04_18425) hslU 2925841..2927172 (-) 1332 WP_001293370.1 HslU--HslV peptidase ATPase subunit -
  IR04_RS18510 (IR04_18430) hslV 2927238..2927795 (-) 558 WP_000208249.1 ATP-dependent protease subunit HslV -
  IR04_RS18515 (IR04_18435) - 2927932..2928483 (-) 552 WP_001884022.1 SPOR domain-containing protein -
  IR04_RS18520 (IR04_18440) cytR 2928655..2929662 (-) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  IR04_RS18525 (IR04_18445) priA 2929968..2932172 (-) 2205 WP_001247858.1 primosomal protein N' Machinery gene
  IR04_RS18530 (IR04_18450) rpmE 2932427..2932645 (+) 219 WP_000643446.1 50S ribosomal protein L31 -
  IR04_RS18535 (IR04_18455) - 2932953..2934230 (+) 1278 WP_001278588.1 malic enzyme-like NAD(P)-binding protein -
  IR04_RS18540 (IR04_18460) metJ 2934341..2934658 (-) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  IR04_RS18545 (IR04_18465) - 2934926..2936092 (+) 1167 WP_000213241.1 O-succinylhomoserine (thiol)-lyase -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82080.38 Da        Isoelectric Point: 8.4853

>NTDB_id=111159 IR04_RS18525 WP_001247858.1 2929968..2932172(-) (priA) [Vibrio cholerae O1 biovar El Tor strain FJ147]
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=111159 IR04_RS18525 WP_001247858.1 2929968..2932172(-) (priA) [Vibrio cholerae O1 biovar El Tor strain FJ147]
ATGCGACCCTCCATTGCCCGAGTTTGCCTACCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGCCAGTTTTATCATTATCCGCTCGGTGAAACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAAGCGGCAGAGCTGACTAGCCATAAAGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCGGTAAAACAAGCGCAAGTTCTCCATTTGTTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAGGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACA
CCGGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTGGTGCCGGAAATTGGTTTAACCCCACAAACCATCAACCGGTTTCG
CCAACGTTTTAATGTCCCCGTCGAAGTCATGCACTCGGCGCTCAACGATACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGCCTGCGTTATCACGCCCGCGATGTGGCTGTGATGCGCGCGCA
TCTGGAGAATATTCCGATTGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCAGTGGTAAATACC
ATCACTTGCAACTGACGCAGCGTGCTGGTAATGCACTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGTCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGCCATGAATGCGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAGATGCGCTGCCACCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGTTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGTAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAGCTTTTAC
TACAAACGCCGAATCGCACCCTGATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGGCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(204-364)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KNQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.572

100

0.439

Multiple sequence alignment