Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   ACNQK5_RS05505 Genome accession   NZ_CP184724
Coordinates   1089721..1092345 (-) Length   874 a.a.
NCBI ID   WP_103112708.1    Uniprot ID   A0A2H6BUU7
Organism   Microcystis aeruginosa NIES-298     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1084721..1097345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNQK5_RS05490 (ACNQK5_05490) - 1085849..1086634 (+) 786 Protein_1084 AAA-like domain-containing protein -
  ACNQK5_RS05495 (ACNQK5_05495) - 1086656..1087986 (-) 1331 Protein_1085 AAA-like domain-containing protein -
  ACNQK5_RS05500 (ACNQK5_05500) - 1088433..1089644 (-) 1212 WP_103112709.1 glycosyltransferase -
  ACNQK5_RS05505 (ACNQK5_05505) pilJ 1089721..1092345 (-) 2625 WP_103112708.1 HAMP domain-containing methyl-accepting chemotaxis protein Machinery gene
  ACNQK5_RS05510 (ACNQK5_05510) - 1092439..1092969 (-) 531 WP_002739344.1 chemotaxis protein CheW -
  ACNQK5_RS05515 (ACNQK5_05515) - 1093769..1093894 (-) 126 WP_080603235.1 DUF4388 domain-containing protein -
  ACNQK5_RS05520 (ACNQK5_05520) hmpF 1094559..1096184 (+) 1626 WP_103112707.1 pilus motility taxis protein HmpF -
  ACNQK5_RS05525 (ACNQK5_05525) - 1096583..1096807 (+) 225 WP_002738436.1 hypothetical protein -

Sequence

Protein


Download         Length: 874 a.a.        Molecular weight: 94863.73 Da        Isoelectric Point: 4.3223

>NTDB_id=1110828 ACNQK5_RS05505 WP_103112708.1 1089721..1092345(-) (pilJ) [Microcystis aeruginosa NIES-298]
MPSGKEYAKLYGKANTAYCQGNLEDAAVIVKEMISKYPDDANVILLQGHIYVGLQQYSLAEGRYEKVLELAKNSANFFEL
VDYARRGLDQIQQSRFEAENGDFMIAATESIAYSEAAFDPSQGFSNSLSGGSQAAAIERQQEVVDWDSGLFNDEDFGEPT
VGTVDSYGDDFGESEETAFSSLILAGSGSHGSQWSGDSADGEPPFPFLEAAEIDPLQEGWGGGLGSTGEATFTADWRENP
VTGRDQELSYVDAPETSTFAIDPELPRRQAAKTDLNQGRVTQKRYEEDEARDFRELKLNDEDLDGFSQLRLTDIGEELGE
SELFTGSGEEALTAGFVPEPRASLNASSAVGEPSFHPSRLEIPDDKRDSHLGPVSERLLGPVVEVNPGKLAFFVNASLGK
KQLILAALAGITPVVLIFAVSTTSWLSAKVAPKTAPIPPAPASLSPWSQPKPAMMLLTGLGTFALTWLGLQLLISQIKRS
LDDLQSQFDHLSEGNFNAKATIYSEDEFGQLANRFNQMAQVVATITTLAQGRAAETERERKNLQRQVIRLLDDVEGAARG
DLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQASTNSESFARNQSSDALRMAEELAVTLNSVQMMTDSI
QRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRDTVSETARKVKRLAEASQEISKIVAVVSQIASRTNLLALNA
SIQAARAGDAGRGFAVVADEVRQLAYRSAKSLKEIEQIVLQIQSETGSVMMAMEEGIQQVIDVTERSEQAKKSLEDIIQV
SNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQQMAGSLQKLVSISRGLLSSVERFKIDSEDN

Nucleotide


Download         Length: 2625 bp        

>NTDB_id=1110828 ACNQK5_RS05505 WP_103112708.1 1089721..1092345(-) (pilJ) [Microcystis aeruginosa NIES-298]
ATGCCGTCAGGGAAAGAATATGCGAAACTCTATGGCAAGGCGAACACCGCCTATTGCCAGGGCAACCTTGAGGATGCCGC
CGTCATTGTCAAAGAAATGATCAGCAAGTATCCCGATGATGCCAACGTCATCCTTTTACAGGGACACATTTATGTCGGAC
TACAACAATACAGCTTGGCCGAGGGACGTTACGAAAAAGTGCTGGAATTGGCCAAAAATTCTGCCAATTTCTTTGAGCTG
GTAGATTATGCTCGACGGGGTCTCGATCAGATTCAGCAATCGCGTTTTGAAGCCGAGAATGGAGATTTTATGATCGCCGC
TACGGAAAGCATTGCCTACAGCGAGGCAGCTTTCGATCCCAGTCAAGGTTTCAGCAATTCTTTGTCCGGGGGGTCGCAAG
CGGCTGCTATCGAGCGACAACAGGAGGTAGTGGACTGGGATAGTGGTCTTTTCAATGACGAGGATTTCGGAGAGCCTACG
GTGGGTACAGTCGATTCCTATGGCGATGATTTTGGGGAATCCGAGGAAACCGCCTTTAGTTCCCTTATCCTGGCTGGTTC
AGGCTCTCACGGTTCCCAGTGGTCCGGGGATTCTGCGGATGGAGAACCCCCTTTCCCCTTTTTGGAAGCAGCGGAGATTG
ACCCGCTGCAGGAAGGGTGGGGCGGTGGGTTAGGATCAACGGGAGAAGCCACTTTTACGGCTGACTGGCGGGAAAATCCC
GTCACCGGTCGAGATCAAGAGTTATCCTATGTCGATGCCCCAGAAACAAGCACTTTTGCCATTGACCCGGAACTGCCCCG
CCGACAAGCGGCGAAAACTGACCTTAATCAGGGCAGAGTGACTCAAAAAAGATACGAGGAGGACGAAGCCAGAGATTTTA
GAGAACTAAAACTCAATGATGAGGATTTAGACGGTTTTTCCCAACTGCGTCTGACGGATATAGGCGAAGAACTGGGGGAA
TCGGAGCTATTTACCGGTTCGGGAGAGGAAGCTTTAACTGCTGGTTTTGTCCCCGAACCCAGGGCGAGCCTTAATGCCAG
TTCTGCTGTGGGAGAGCCTAGTTTTCATCCTTCTCGTCTAGAAATCCCCGATGACAAAAGAGATAGTCATCTTGGCCCTG
TTTCCGAGCGCCTGCTCGGGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGCCTTTTTTGTCAACGCTTCTTTGGGCAAA
AAACAACTAATTTTAGCGGCTTTAGCGGGTATTACCCCAGTGGTGTTAATTTTTGCCGTTAGCACCACCTCTTGGCTTTC
TGCAAAAGTTGCCCCCAAAACAGCCCCAATACCACCAGCACCAGCCAGCCTTTCTCCTTGGAGTCAACCGAAACCAGCAA
TGATGTTATTAACTGGATTGGGAACCTTTGCCCTAACTTGGCTAGGTTTACAGTTGCTAATTAGTCAAATTAAGCGCAGT
CTCGACGATTTGCAAAGCCAATTCGATCATCTTTCGGAAGGCAATTTTAACGCTAAAGCGACGATCTACTCGGAAGATGA
ATTTGGTCAACTGGCCAATCGTTTCAATCAGATGGCCCAGGTGGTGGCAACTATCACCACCTTGGCCCAAGGCCGCGCCG
CCGAAACGGAACGGGAACGGAAAAATCTACAAAGGCAAGTGATTCGACTTCTCGATGATGTGGAAGGAGCGGCTCGGGGG
GATCTGACAGTGGAAGCGGAGGTAAGTGCCGATGTTTTAGGGGCGGTGGCCGATGCTTTTAATTTGACGATCCAAAACCT
GCGCGAAATTGTCCGACAGGTGAAAAAAGCGGCAAAACAGGTGAATCAAGCCAGTACCAATAGTGAATCTTTCGCCCGCA
ATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAGTTGGCCGTCACCCTTAATTCGGTACAGATGATGACCGACTCAATT
CAAAGGGTGGCAGAAAACGCCAGAGAAGCCGAAGAAGTCGCCCGCACTTCCTCGATTACCGCTCTCAAGGGGGGCGATTC
CGTGGAAAGAACCGTGGCGGGAATTCTGCAAATCCGCGACACGGTATCAGAAACGGCGCGAAAAGTCAAGCGTTTGGCAG
AAGCTTCCCAGGAAATCTCGAAAATTGTCGCAGTAGTCTCTCAGATTGCCTCGCGAACCAATTTATTGGCTCTTAATGCC
TCGATTCAAGCGGCGCGAGCAGGAGATGCAGGCCGCGGTTTTGCGGTGGTGGCCGATGAGGTACGTCAGTTAGCCTATAG
ATCGGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGATTCAATCGGAAACGGGTTCGGTAATGATGGCAATGG
AGGAAGGGATTCAACAGGTTATCGATGTCACCGAGCGCTCGGAACAGGCCAAGAAGTCCCTAGAAGATATTATCCAAGTT
TCTAACCGCATCGATACGCTGGTGCGATCGATTACCGCCGATACGGTCAAACAACGGGAAAATTCCCTCAATGTCGCCCA
AGTTATGCAGTCGGTGGAGTTAACCGCTCAGGAAACTTCCCAAGAATCTCAACAGATGGCGGGTTCTTTGCAAAAATTGG
TGAGTATCTCCCGGGGGCTGCTTTCTTCGGTAGAACGGTTTAAGATAGACTCGGAAGATAATTAA

Domains

Predicted by InterproScan.

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(652-832)

HAMP domain (HAMP)

(472-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2H6BUU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

49.194

100

0.524

Multiple sequence alignment