Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACN08A_RS11875 Genome accession   NZ_CP184653
Coordinates   2352848..2354218 (+) Length   456 a.a.
NCBI ID   WP_047333966.1    Uniprot ID   A0A200I045
Organism   Enterococcus cecorum strain 2023EC-GS-SDAU-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2347848..2359218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08A_RS11850 (ACN08A_11890) - 2349201..2350448 (-) 1248 WP_311897533.1 glutamate-5-semialdehyde dehydrogenase -
  ACN08A_RS11855 (ACN08A_11895) proB 2350464..2351267 (-) 804 WP_311897561.1 glutamate 5-kinase -
  ACN08A_RS11860 (ACN08A_11900) - 2351400..2351567 (-) 168 WP_158409720.1 hypothetical protein -
  ACN08A_RS11865 (ACN08A_11905) - 2351582..2352091 (-) 510 WP_311897532.1 hypothetical protein -
  ACN08A_RS11870 (ACN08A_11910) - 2352314..2352835 (+) 522 WP_026209954.1 hypothetical protein -
  ACN08A_RS11875 (ACN08A_11915) radA 2352848..2354218 (+) 1371 WP_047333966.1 DNA repair protein RadA Machinery gene
  ACN08A_RS11880 (ACN08A_11920) - 2354415..2355521 (+) 1107 WP_420285340.1 PIN/TRAM domain-containing protein -
  ACN08A_RS11885 (ACN08A_11925) - 2355561..2356118 (+) 558 WP_047241735.1 dTDP-4-dehydrorhamnose 3,5-epimerase family protein -
  ACN08A_RS11890 (ACN08A_11930) - 2356379..2356684 (+) 306 WP_087213966.1 hypothetical protein -
  ACN08A_RS11895 (ACN08A_11935) - 2356759..2357067 (+) 309 WP_311897530.1 GNAT family N-acetyltransferase -
  ACN08A_RS11900 (ACN08A_11940) - 2357196..2358500 (+) 1305 WP_089202021.1 ISL3 family transposase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49967.80 Da        Isoelectric Point: 7.7241

>NTDB_id=1110616 ACN08A_RS11875 WP_047333966.1 2352848..2354218(+) (radA) [Enterococcus cecorum strain 2023EC-GS-SDAU-1]
MAKKAKTQFECQHCGYISPRYLGKCPNCNSWNSMMEVKIENTTDVRQAVSFTGIKSQPTKITEVVPKKTPRVQTEIGELN
RVLGGGVVPGSLILLGGDPGIGKSTLLLQVSEQLTKAHGKVLYVSGEESAEQIKMRAERLGVLHESFYVYAENDMEAIRQ
AIEQLSPDYVIIDSIQTMIQPSISSVAGSVSQVRETTAELMRIAKSNQIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GERQQSFRILRAVKNRFGSTNEIGIFEMHEDGLHEVINPSQVFLEERLQGATGSAIVVTMEGTRPLLVEIQALITPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGMLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQATDCFIGEIGLTG
EIRRVNAIEQRIKEVQKLGFKRVFVPKNNLANLEIPAGIEVIGVSTLAETMKKVFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1110616 ACN08A_RS11875 WP_047333966.1 2352848..2354218(+) (radA) [Enterococcus cecorum strain 2023EC-GS-SDAU-1]
GTGGCAAAAAAAGCAAAAACACAATTTGAGTGTCAACATTGTGGCTATATTTCCCCACGTTATTTAGGGAAATGTCCGAA
TTGTAATAGTTGGAACTCAATGATGGAAGTCAAAATCGAAAATACCACTGATGTCCGTCAAGCCGTTTCGTTTACCGGGA
TTAAGAGCCAACCAACTAAAATTACCGAAGTAGTGCCGAAAAAAACACCGCGCGTCCAAACAGAAATTGGTGAGTTGAAT
CGCGTGCTAGGTGGTGGTGTGGTGCCAGGTTCTTTGATTTTACTAGGAGGCGATCCGGGTATTGGCAAGTCGACACTGTT
ATTACAAGTGTCTGAGCAGCTAACCAAAGCCCACGGGAAAGTCTTGTACGTCTCAGGGGAAGAAAGTGCCGAGCAAATCA
AGATGCGTGCGGAACGTCTAGGGGTCTTGCATGAGTCCTTTTATGTCTATGCGGAAAATGATATGGAGGCCATTCGTCAA
GCCATCGAACAACTTTCGCCTGATTATGTGATTATTGATTCGATTCAAACCATGATTCAGCCAAGCATTTCTTCTGTGGC
GGGAAGTGTCAGTCAGGTCCGCGAAACCACCGCTGAGTTGATGCGCATAGCCAAGAGTAATCAGATTGCCATTTTTATCG
TTGGTCATGTGACAAAAGAAGGAGCACTAGCAGGTCCTAGAATGTTAGAACACATGGTTGATACCGTGTTATATTTTGAA
GGAGAACGCCAACAGTCCTTTAGAATTTTGCGTGCGGTGAAAAATCGTTTCGGTTCGACCAATGAAATCGGCATTTTCGA
GATGCATGAAGATGGGTTGCATGAAGTCATCAATCCTTCGCAAGTCTTTTTAGAAGAACGTCTGCAAGGAGCGACTGGTT
CGGCAATCGTGGTGACGATGGAAGGAACGCGCCCATTATTAGTGGAGATTCAAGCTTTGATTACCCCCACCATGTTTGGA
AATGCCAAGCGTACCACGACAGGTCTAGACTTTAATCGGGTATCTTTAATTATGGCGGTCTTGGAAAAGCGTGCCGGAAT
GTTATTGCAAAATCAAGATGCCTATTTAAAAGCAGCCGGTGGTGTGAAGTTAAACGAGCCAGCAATCGATTTAGCCATTG
CGGTGAGTATTGCCTCTAGTTACAAGGAAAAAGGCACCCAGGCAACGGATTGTTTTATCGGTGAAATTGGCTTGACCGGG
GAAATCAGACGCGTCAATGCCATCGAACAACGCATCAAAGAAGTGCAAAAGTTAGGCTTTAAACGGGTATTTGTACCTAA
AAATAATTTAGCTAATTTAGAAATTCCAGCGGGTATTGAAGTTATCGGGGTTTCTACTTTGGCTGAAACGATGAAAAAAG
TCTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A200I045

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

70.769

99.781

0.706
  radA Streptococcus pneumoniae D39

70.769

99.781

0.706
  radA Streptococcus pneumoniae R6

70.769

99.781

0.706
  radA Streptococcus pneumoniae TIGR4

70.769

99.781

0.706
  radA Streptococcus mitis NCTC 12261

70.769

99.781

0.706
  radA Streptococcus mitis SK321

70.769

99.781

0.706
  radA Bacillus subtilis subsp. subtilis str. 168

62.857

99.781

0.627

Multiple sequence alignment