Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACN8ZM_RS15080 Genome accession   NZ_CP184468
Coordinates   3223575..3225080 (+) Length   501 a.a.
NCBI ID   WP_043187322.1    Uniprot ID   A0A228IW03
Organism   Burkholderia aenigmatica strain ST2120     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3218575..3230080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN8ZM_RS15055 (ACN8ZM_15055) kdsD 3219818..3220801 (+) 984 WP_043187332.1 arabinose 5-phosphate isomerase KdsD -
  ACN8ZM_RS15060 (ACN8ZM_15060) - 3220801..3221337 (+) 537 WP_043187330.1 KdsC family phosphatase -
  ACN8ZM_RS15065 (ACN8ZM_15065) lptC 3221341..3221955 (+) 615 WP_043187328.1 LPS export ABC transporter periplasmic protein LptC -
  ACN8ZM_RS15070 (ACN8ZM_15070) lptA 3221976..3222641 (+) 666 WP_043187325.1 lipopolysaccharide transport periplasmic protein LptA -
  ACN8ZM_RS15075 (ACN8ZM_15075) lptB 3222638..3223414 (+) 777 WP_034189275.1 LPS export ABC transporter ATP-binding protein -
  ACN8ZM_RS15080 (ACN8ZM_15080) rpoN/rpoN1 3223575..3225080 (+) 1506 WP_043187322.1 RNA polymerase factor sigma-54 Machinery gene
  ACN8ZM_RS15085 (ACN8ZM_15085) hpf 3225239..3225598 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACN8ZM_RS15090 (ACN8ZM_15090) - 3225767..3226306 (+) 540 WP_080741751.1 PTS sugar transporter subunit IIA -
  ACN8ZM_RS15095 (ACN8ZM_15095) hprK 3226436..3227404 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  ACN8ZM_RS15100 (ACN8ZM_15100) rapZ 3227480..3228388 (+) 909 WP_043187318.1 RNase adapter RapZ -
  ACN8ZM_RS15105 (ACN8ZM_15105) - 3228416..3229051 (+) 636 WP_043187316.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54831.91 Da        Isoelectric Point: 4.8740

>NTDB_id=1108407 ACN8ZM_RS15080 WP_043187322.1 3223575..3225080(+) (rpoN/rpoN1) [Burkholderia aenigmatica strain ST2120]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTS
TNSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1108407 ACN8ZM_RS15080 WP_043187322.1 3223575..3225080(+) (rpoN/rpoN1) [Burkholderia aenigmatica strain ST2120]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCGCAGAACACCGAGCCCACTTCG
ACGAACAGCAGCAGCTCGTCGAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAGTACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGTGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGATGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGCTCCGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACACTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGCTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAACTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAACAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAAAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGAGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTACCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A228IW03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.984

100

0.687

Multiple sequence alignment