Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M1B74_RS06635 Genome accession   NZ_CP184347
Coordinates   1664376..1665743 (-) Length   455 a.a.
NCBI ID   WP_027324506.1    Uniprot ID   W4PHY4
Organism   Bacteroides pyogenes strain LC-305-2021     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1659376..1670743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1B74_RS06605 (M1B74_06600) - 1659700..1659879 (-) 180 Protein_1279 ATP-binding protein -
  M1B74_RS06610 (M1B74_06605) - 1659909..1660197 (-) 289 Protein_1280 IS21 family transposase -
  M1B74_RS06615 (M1B74_06610) - 1660508..1660954 (+) 447 WP_148295155.1 RNA polymerase sigma factor -
  M1B74_RS06620 (M1B74_06615) - 1660939..1661520 (-) 582 WP_420187995.1 hypothetical protein -
  M1B74_RS06625 (M1B74_06620) - 1661974..1662573 (-) 600 WP_021646416.1 response regulator transcription factor -
  M1B74_RS06630 (M1B74_06625) - 1662676..1664343 (-) 1668 WP_418987800.1 NAD(P)/FAD-dependent oxidoreductase -
  M1B74_RS06635 (M1B74_06630) radA 1664376..1665743 (-) 1368 WP_027324506.1 DNA repair protein RadA Machinery gene
  M1B74_RS06640 (M1B74_06635) - 1666052..1667101 (-) 1050 WP_212122269.1 asparaginase -
  M1B74_RS06645 (M1B74_06640) thrA 1667530..1669965 (+) 2436 WP_420187996.1 bifunctional aspartate kinase/homoserine dehydrogenase I -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50058.77 Da        Isoelectric Point: 6.4043

>NTDB_id=1108298 M1B74_RS06635 WP_027324506.1 1664376..1665743(-) (radA) [Bacteroides pyogenes strain LC-305-2021]
MAKEKTVYVCSNCGQGSPKWVGKCPSCGEWNTYVEEIVRKEPVSRRPVSGIETPKAKPLALSEIEADDEPRIDMHDEELN
RVLGGGLVPGSLVLIGGEPGIGKSTLVMQTVLRMPERKILYVSGEESARQLKLRADRLSRVSNDCLIVCETSLEQIYVHI
KNTNPDLVIIDSIQTISTETIESSPGSVAQVRECSASILRFAKETHTPVLLIGHINKEGSIAGPKVLEHIVDTVLQFEGD
QHYMYRILRSIKNRFGSTAELGIYEMRQDGLRQVSNPSELLLSQDHEGMSGVAVASAIEGVRPFLIETQALVSTAAYGTP
QRSATGFDIRRMNMLLAVLEKRVGFKLAQKDVFLNIAGGLKVNDPAIDLAVISAILSSNMDAPVEREICMAGEIGLSGEI
RPVNRIEQRIGEAEKLGFKQFLLPKYNLQGINTQKLKIELVPVRKVEEAFRALFG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1108298 M1B74_RS06635 WP_027324506.1 1664376..1665743(-) (radA) [Bacteroides pyogenes strain LC-305-2021]
ATGGCTAAAGAGAAAACAGTGTATGTATGCAGCAACTGCGGACAGGGTTCGCCGAAATGGGTCGGCAAATGTCCGTCGTG
CGGAGAATGGAACACGTATGTGGAAGAGATTGTGCGGAAAGAGCCGGTAAGCCGGCGTCCCGTGTCGGGTATCGAGACCC
CGAAGGCGAAACCGCTTGCATTGAGCGAGATAGAAGCGGACGACGAACCGCGCATCGACATGCACGACGAAGAGTTGAAC
CGGGTGTTGGGAGGCGGACTTGTGCCGGGTTCGCTCGTACTGATAGGCGGTGAGCCGGGCATCGGGAAGTCGACGCTGGT
GATGCAAACCGTGCTTCGTATGCCGGAACGGAAAATCCTCTACGTCTCCGGCGAAGAGAGCGCACGGCAGTTGAAACTTC
GTGCCGACCGCCTTTCCCGCGTGTCGAACGATTGCCTCATCGTATGCGAGACTTCGCTGGAGCAAATATACGTGCACATC
AAGAATACCAACCCCGATTTAGTCATCATCGACTCCATCCAGACCATCTCCACCGAGACGATCGAATCTTCGCCCGGAAG
CGTCGCCCAGGTACGGGAATGTTCGGCCTCCATCCTGCGGTTTGCCAAAGAAACCCATACGCCGGTGTTGCTGATCGGGC
ATATCAATAAGGAAGGAAGCATCGCAGGGCCGAAAGTGCTGGAGCACATCGTCGACACCGTTCTTCAGTTCGAAGGCGAC
CAACACTATATGTACCGCATCCTGCGAAGCATCAAAAACCGTTTCGGCAGCACGGCGGAGCTGGGCATTTACGAGATGCG
GCAGGACGGACTGCGGCAAGTGAGCAACCCGTCGGAACTGTTGCTGAGCCAAGACCATGAAGGGATGAGCGGAGTGGCGG
TGGCCTCCGCCATCGAAGGCGTGCGCCCGTTTCTGATAGAGACGCAGGCTCTGGTAAGCACGGCGGCTTACGGAACGCCC
CAACGCTCGGCTACGGGATTCGACATACGTAGGATGAACATGTTGCTGGCGGTGCTCGAAAAGCGCGTCGGCTTCAAACT
TGCGCAGAAAGACGTGTTCCTCAACATTGCCGGCGGACTGAAAGTGAACGACCCCGCCATCGACTTGGCAGTCATCAGCG
CTATCTTGTCGTCGAACATGGACGCTCCCGTCGAACGGGAAATCTGCATGGCGGGAGAAATCGGACTTTCGGGAGAGATA
CGCCCGGTGAACCGCATCGAGCAACGCATCGGTGAAGCGGAAAAGCTGGGTTTCAAACAGTTTCTGCTGCCCAAATACAA
CCTGCAAGGGATAAATACCCAAAAACTGAAAATTGAACTGGTTCCGGTGAGGAAGGTGGAAGAGGCGTTCAGGGCGCTAT
TCGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-427)

AAA domain (AAA_25)

(67-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W4PHY4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.545

100

0.51
  radA Streptococcus mitis SK321

47.692

100

0.477
  radA Streptococcus mitis NCTC 12261

47.692

100

0.477
  radA Streptococcus pneumoniae Rx1

47.692

100

0.477
  radA Streptococcus pneumoniae D39

47.692

100

0.477
  radA Streptococcus pneumoniae R6

47.692

100

0.477
  radA Streptococcus pneumoniae TIGR4

47.692

100

0.477

Multiple sequence alignment