Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACN19N_RS12695 Genome accession   NZ_CP184160
Coordinates   2640868..2641671 (-) Length   267 a.a.
NCBI ID   WP_269228782.1    Uniprot ID   A0A9E9NMZ7
Organism   Acinetobacter sp. LF10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2635868..2646671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN19N_RS12675 (ACN19N_12675) - 2636923..2637621 (-) 699 WP_269228779.1 YfgM family protein -
  ACN19N_RS12680 (ACN19N_12680) hisS 2637653..2638945 (-) 1293 WP_293743625.1 histidine--tRNA ligase -
  ACN19N_RS12685 (ACN19N_12685) ispG 2638942..2640057 (-) 1116 WP_004664888.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACN19N_RS12690 (ACN19N_12690) - 2640080..2640877 (-) 798 WP_269238477.1 helix-turn-helix domain-containing protein -
  ACN19N_RS12695 (ACN19N_12695) pilF 2640868..2641671 (-) 804 WP_269228782.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACN19N_RS12700 (ACN19N_12700) rlmN 2641689..2642924 (-) 1236 WP_293743619.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACN19N_RS12705 (ACN19N_12705) ndk 2643046..2643477 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  ACN19N_RS12710 (ACN19N_12710) iscX 2643586..2643786 (-) 201 WP_004664895.1 Fe-S cluster assembly protein IscX -
  ACN19N_RS12715 (ACN19N_12715) - 2643881..2644432 (-) 552 WP_269228784.1 phosphatase PAP2 family protein -
  ACN19N_RS12720 (ACN19N_12720) - 2644457..2645743 (-) 1287 WP_420002392.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30219.21 Da        Isoelectric Point: 9.4465

>NTDB_id=1108021 ACN19N_RS12695 WP_269228782.1 2640868..2641671(-) (pilF) [Acinetobacter sp. LF10]
MNTTRLKTIIVPTVVFTVLGLTACQSATGLKKDPEKAVQVRSQLAAEYIRTGDLDSAKRSLDQALNIDSRDANANMMMGI
LLQQEGSKSNMEKADAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTRAGATLGYEQRFQSLENLGRIYLKLGD
VANAEKAFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARSSADQLGMQVLVN
QLRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1108021 ACN19N_RS12695 WP_269228782.1 2640868..2641671(-) (pilF) [Acinetobacter sp. LF10]
TTGAATACAACTCGACTAAAAACGATTATAGTGCCAACTGTTGTGTTTACAGTACTAGGGTTAACTGCTTGCCAATCTGC
TACAGGATTAAAAAAAGATCCAGAAAAGGCAGTGCAAGTTCGTTCTCAACTGGCTGCTGAATATATTCGTACAGGTGATT
TGGATTCCGCTAAACGTTCTCTTGATCAAGCTTTAAATATTGATTCGCGTGATGCAAACGCCAATATGATGATGGGGATT
CTACTGCAACAAGAAGGCAGTAAGTCCAATATGGAAAAAGCAGATGCTTATTTTAAGCGTGCGATTTCTTCAGAACCAGA
TAATGCACAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAAGCGATTGAGCAATTTACCC
GTGCAGGTGCAACCTTAGGTTATGAACAGCGATTTCAATCGTTGGAAAACTTAGGGCGAATCTATTTAAAGTTAGGCGAT
GTTGCGAATGCTGAAAAAGCATTCAAACAAGCGCTACAAGCAAATCGTAACGCAACTATTTCAATGTTAGAGTTGGCAGA
AATTTTCTATTTGCAACAGAATAATAGAGATGCATCGCAACTATACGAACAGTATGTTCGTAATGTGGGGCAAAAAAACC
AAGGTGCGCGTGCACTTTGGATTGGTATACGCATTGCTCGATCGAGTGCGGATCAATTAGGAATGCAAGTGTTAGTGAAT
CAATTACGAGCATTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
ATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(109-176)

Tetratricopeptide repeat (TPR_8)

(88-108)

Tetratricopeptide repeat (TPR_8)

(43-69)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.528

100

0.775
  pilF Acinetobacter baylyi ADP1

67.816

97.753

0.663

Multiple sequence alignment