Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACGHC0_RS02515 Genome accession   NZ_AP035783
Coordinates   542008..543597 (+) Length   529 a.a.
NCBI ID   WP_368353858.1    Uniprot ID   -
Organism   Acidovorax sacchari strain HC-19     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537008..548597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHC0_RS02490 (AVXHC19_05020) argS 537338..539047 (+) 1710 WP_415267346.1 arginine--tRNA ligase -
  ACGHC0_RS02495 (AVXHC19_05030) - 539062..539712 (+) 651 WP_415267347.1 SPOR domain-containing protein -
  ACGHC0_RS02500 (AVXHC19_05040) - 539786..540442 (+) 657 WP_368353855.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACGHC0_RS02505 (AVXHC19_05050) lptA 540564..541214 (+) 651 WP_368353856.1 lipopolysaccharide transport periplasmic protein LptA -
  ACGHC0_RS02510 (AVXHC19_05060) lptB 541217..542008 (+) 792 WP_368353857.1 LPS export ABC transporter ATP-binding protein -
  ACGHC0_RS02515 (AVXHC19_05070) rpoN/rpoN1 542008..543597 (+) 1590 WP_368353858.1 RNA polymerase factor sigma-54 Machinery gene
  ACGHC0_RS02520 (AVXHC19_05080) - 543623..544168 (-) 546 WP_368353859.1 TspO/MBR family protein -
  ACGHC0_RS02525 (AVXHC19_05090) - 544209..546056 (-) 1848 WP_415267348.1 methyl-accepting chemotaxis protein -
  ACGHC0_RS02530 (AVXHC19_05100) - 546212..547537 (+) 1326 WP_368353861.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACGHC0_RS02535 (AVXHC19_05110) - 547527..548009 (+) 483 WP_368353862.1 putative toxin-antitoxin system toxin component, PIN family -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 57936.16 Da        Isoelectric Point: 4.6010

>NTDB_id=110794 ACGHC0_RS02515 WP_368353858.1 542008..543597(+) (rpoN/rpoN1) [Acidovorax sacchari strain HC-19]
MKPGLSLRISQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERNPDEAPREEFGLPQADAPVSDADRGAEESLY
TPASGATEAAASSPGEGASEGPDAPEVPDAPDWEGDGTVEMAPDDGEWGGEAPARTRNQMGDEDDTDATELAREHESLTD
YLHRQALSLRLSEVDRAALRFLIESLNDDGYLDESLEALAVALAGEEDPEQVEELVHRFTVALRLLQSLEPVGVGARGLS
ECMALQLRALQEDGDAEPEVLQAALRICSQPLEMLARRDVRRLAQGCGASEECTRAAMALIARLEPRPGRAFAQVERNII
VPDVIVKKSSGRGAAHAFTVQLNPDVMPRLRVHDIYAGALRGNRGGEGHQALQQRLQEARWFIKNIQQRFDTILRVSRAI
VDRQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQF
VASESPAKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=110794 ACGHC0_RS02515 WP_368353858.1 542008..543597(+) (rpoN/rpoN1) [Acidovorax sacchari strain HC-19]
ATGAAGCCGGGACTGTCGCTGCGCATCTCGCAGCATCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACGCTGGAGCTGTCGCAGGAAGTCGAGCAGATGCTCGACGAGAACCCCTTCCTAGAGCGCAATCCGGACGAGG
CGCCGCGCGAGGAGTTCGGGTTGCCGCAGGCGGATGCGCCGGTGAGCGATGCCGACCGCGGTGCGGAGGAGTCCCTCTAT
ACGCCGGCCAGCGGGGCCACGGAGGCGGCAGCCTCGTCGCCCGGCGAGGGCGCATCCGAAGGCCCGGACGCGCCGGAGGT
GCCCGACGCGCCGGACTGGGAGGGCGACGGCACCGTCGAGATGGCACCCGACGACGGCGAGTGGGGCGGCGAGGCGCCCG
CACGCACCCGCAACCAGATGGGGGACGAGGACGACACGGACGCCACCGAACTCGCGCGCGAGCACGAGTCGCTTACCGAT
TACCTGCACCGGCAGGCGCTGTCGCTGCGCCTGTCGGAGGTGGACCGCGCGGCGCTCCGCTTCCTGATCGAATCCCTGAA
CGACGACGGCTACCTCGACGAGTCGCTCGAAGCCCTGGCCGTGGCCCTGGCGGGCGAGGAAGACCCCGAGCAGGTGGAGG
AACTGGTGCACCGCTTCACGGTGGCGCTGCGGCTCTTGCAGAGCCTGGAGCCCGTGGGCGTGGGCGCGCGCGGCCTGAGC
GAATGCATGGCGCTGCAGCTGCGGGCCCTGCAGGAAGATGGCGACGCGGAACCCGAAGTGCTGCAGGCGGCGCTGCGCAT
CTGCAGTCAGCCGCTGGAGATGCTGGCGCGGCGCGACGTGCGCCGGCTCGCCCAGGGCTGCGGTGCCAGCGAGGAGTGCA
CGCGCGCGGCGATGGCGCTCATCGCACGGCTGGAACCGCGGCCGGGCCGGGCTTTCGCGCAGGTCGAGCGCAACATCATC
GTTCCCGACGTGATCGTGAAGAAGAGCAGCGGCCGCGGGGCCGCGCATGCGTTCACGGTGCAGCTCAATCCGGACGTCAT
GCCGCGGCTGCGCGTGCACGACATCTACGCTGGCGCGCTGCGCGGCAACCGCGGCGGCGAGGGGCACCAGGCGCTGCAGC
AGCGGCTGCAGGAGGCACGCTGGTTCATCAAGAACATCCAGCAGCGGTTCGACACCATCCTGCGCGTCTCGCGCGCCATC
GTGGACCGCCAGAAGAACTTCTTCGTGCATGGCGAGCTGGCCATGCGGCCGCTCGTGCTGCGCGACATCGCCGACGAGCT
GGGACTGCACGAGTCCACCATCAGCCGGGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGGACCTACGAGCTGAAGT
ATTTCTTCGGCTCGGGGCTGGGCACGGAGACGGGCGGCAATGCGTCCAGCACGGCCGTGCGTGCGCTCATCAAGCAGTTC
GTCGCATCGGAGAGCCCGGCCAAGCCGCTGTCGGACAGCCAGATCGCGGAGATGCTCAAGGAGCAGGGCATCGAGTGCGC
GCGCCGCACGGTCGCCAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCCAACCTGCGCAAGGCGCTGTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(149-356)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.642

100

0.527

Multiple sequence alignment