Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   HZ99_RS19700 Genome accession   NZ_CP008896
Coordinates   4403736..4405955 (-) Length   739 a.a.
NCBI ID   WP_038445417.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain UK4     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4398736..4410955
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZ99_RS19680 (HZ99_20115) hslU 4398910..4400247 (-) 1338 WP_038445413.1 ATP-dependent protease ATPase subunit HslU -
  HZ99_RS19685 (HZ99_20120) hslV 4400286..4400816 (-) 531 WP_017738857.1 ATP-dependent protease subunit HslV -
  HZ99_RS19690 (HZ99_20125) - 4401073..4401774 (-) 702 WP_038445415.1 SPOR domain-containing protein -
  HZ99_RS19695 (HZ99_20130) argS 4401776..4403512 (-) 1737 WP_038448133.1 arginine--tRNA ligase -
  HZ99_RS19700 (HZ99_20135) priA 4403736..4405955 (-) 2220 WP_038445417.1 primosomal protein N' Machinery gene
  HZ99_RS19705 (HZ99_20140) rpmE 4406158..4406388 (+) 231 WP_029296053.1 50S ribosomal protein L31 -
  HZ99_RS19710 (HZ99_20145) - 4406403..4407209 (+) 807 WP_038445419.1 thermonuclease family protein -
  HZ99_RS19715 (HZ99_20150) - 4407393..4408661 (+) 1269 WP_038445421.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81967.45 Da        Isoelectric Point: 8.4355

>NTDB_id=110793 HZ99_RS19700 WP_038445417.1 4403736..4405955(-) (priA) [Pseudomonas fluorescens strain UK4]
MPDAILRLALPSPLRRLFDYRAPAGVVRSQLQPGMRVRVPFGRREMIGILIEVADHSEVPPEKLKPALAILDATPPLPAS
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAETRQERFWSITPGARLDDPRIARAPRQREVLATLAQHPHGVAHPLL
SKLMLSKDSLDLLLAKDLVQVEIRRHAPGERHEHWLAQPELPLNPEQRAAFEAISASADSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLESWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECNRCDARMTVHQRSAELRCHHCGHVERVPRHC
PQCGKVDLRPVGAGTERAEERLGILFPDFPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGRVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEGFLDEACSVAERLLGELGLSGIELLGPVPAPMERRAGRYRAQLLLQATARAPLHRLLSSWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=110793 HZ99_RS19700 WP_038445417.1 4403736..4405955(-) (priA) [Pseudomonas fluorescens strain UK4]
GTGCCCGACGCCATTTTGCGCCTCGCCCTGCCCTCGCCCCTGCGCCGCCTGTTCGACTATCGGGCACCCGCGGGCGTGGT
GCGGTCGCAGTTGCAGCCGGGGATGCGCGTACGCGTGCCGTTCGGCCGACGGGAGATGATCGGGATCCTGATCGAAGTGG
CGGATCATAGCGAAGTGCCACCAGAAAAGCTCAAGCCGGCCCTGGCCATCCTGGACGCCACGCCGCCGCTGCCGGCGTCG
CTGTTCAAGCTGTGCCTGTGGACTTCCCAGTATTACCAGCACAGCCTGGGCGATACGTTGAGCTGGGCATTGCCGGTGCT
GTTGCGCCAGGGCGAGCTGGCCGAGACCCGCCAGGAGCGCTTCTGGTCGATAACGCCCGGTGCACGCCTGGACGACCCAC
GTATCGCCCGCGCCCCACGCCAGCGCGAGGTCCTGGCCACACTGGCGCAGCACCCCCATGGCGTTGCTCACCCACTGCTG
AGCAAACTGATGTTGAGCAAGGACAGCCTCGACCTGCTGCTGGCCAAGGACCTGGTGCAGGTGGAAATCCGCCGCCACGC
CCCCGGCGAGCGCCATGAACACTGGCTGGCCCAACCGGAGCTGCCGCTGAACCCGGAACAGCGCGCCGCGTTCGAGGCCA
TCAGCGCCAGCGCCGACAGCTTCCACGCATTCCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACCGAAGTCTATTTGCAA
TTGATCCGCGAAACCCTGGAAGCCGGCAAGCAGGCCCTGGTGCTGATCCCCGAGATTAACCTCGGCCCCCAGACCCTGGC
GCGCTTCGAACAACGCTTCAATGCGCGGATCGCCCTGGTGCATTCGGCCGTCAATGACCGCGAGCGCCTGGAATCCTGGC
TGGCCGCCCGGGACGGCGAGGCCGACATTATTATCGGTACCCGCTCGGCCCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGCTCCTACAAACAGCAGGAAGGCCTGCGCTACCACGCCCGCGACCTGGCCCTGGT
GCGCGCACGCCAGGAAAACATTCCGATTGTGCTGGGCTCGGCCACGCCCTCCCTGGAAAGCCTGCATAACGCGTACACCG
GACGCTACGGCCTGCTGCGCCTGAACGAACGGGCGGGCGGGGCCAAACAACCGCGCTTCCTGCGCCTGGATGTAAAAAGT
CGCCCACTCGACAGCGGCATTTCCGGGCCGATGCAACAGGCCATCGGCCAGACCCTGGCGGCCGGCCAGCAAGTGCTGGT
GTTCCTCAATCGCCGGGGCTTTGCGCCGACACTGCTGTGCCACGACTGTGGCTGGATGTCCGAGTGCAACCGCTGCGATG
CGCGGATGACCGTGCACCAGCGCTCCGCCGAATTGCGCTGCCATCACTGCGGCCATGTGGAGCGGGTCCCGCGTCATTGC
CCGCAGTGCGGCAAAGTCGACCTGCGGCCAGTAGGCGCGGGCACCGAACGCGCCGAGGAGCGCCTGGGGATTCTGTTCCC
GGACTTCCCGGTACTGCGCGTAGACCGCGACAGTACGTCGCGCAAGGACGCCATGAACCAACTGTTCGCCACCATCCAGA
AAGGCCAGCCGTGCATTTTGGTAGGCACGCAAATGCTTGCCAAAGGGCACCACTTTCCACGTGTGACCCTGGTCTCGATC
CTGGATGCCGACGGCGGGCTGTTCTCCGGCGACTTTCGCGCCAGCGAACGCATGGCGCAGTTGATCGTGCAGGTTGCTGG
CCGCGCCGGGCGTGCCGAGGAGCCTGGCCGAGTGATCATCCAGACCCACCTGGCCGACCATCCATTGCTGATTCAACTGA
CAGAACAAGGCTATTTTGCCTTCGCCGAACAGGCCTTGAGCGAACGTCGCGCAGCCGGCCTGCCACCCTTTGCGCACCTG
GCGCTGTTGCGCGCCGAGGCCCATAAACCGGGGCAGGCCGAAGGTTTTCTGGATGAGGCCTGCAGCGTGGCTGAACGTTT
GCTCGGTGAACTGGGTCTGAGCGGCATCGAGTTGCTGGGCCCGGTGCCAGCACCGATGGAACGCCGCGCCGGACGTTATC
GCGCGCAGCTACTCTTGCAAGCCACCGCTCGTGCACCGCTGCATCGGCTATTAAGTAGCTGGTTACTTGCTCTGGAGCAA
ATGCCCAGCGGACGGCAGGTGCGATGGTCGTTGGATGTCGATCCGGTCGATTTGTACTGA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

DEAD/DEAH box helicase (DEAD)

(206-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

46.837

100

0.471

Multiple sequence alignment