Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACN9KP_RS01770 Genome accession   NZ_CP183947
Coordinates   325116..325553 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 320116..330553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN9KP_RS01730 (ACN9KP_01730) - 320696..320860 (-) 165 WP_370648487.1 molybdopterin dinucleotide binding domain-containing protein -
  ACN9KP_RS01735 (ACN9KP_01735) - 320974..322176 (+) 1203 WP_032857854.1 GTP-binding protein -
  ACN9KP_RS01740 (ACN9KP_01740) - 322241..323377 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  ACN9KP_RS01745 (ACN9KP_01745) - 323392..323826 (+) 435 WP_003156592.1 RDD family protein -
  ACN9KP_RS01750 (ACN9KP_01750) - 323943..324218 (+) 276 WP_369719109.1 YckD family protein -
  ACN9KP_RS01755 (ACN9KP_01755) - 324322..324438 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  ACN9KP_RS01760 (ACN9KP_01760) - 324437..324655 (+) 219 Protein_306 family 1 glycosylhydrolase -
  ACN9KP_RS01765 (ACN9KP_01765) nin/comJ 324696..325094 (-) 399 WP_014304317.1 competence protein ComJ Regulator
  ACN9KP_RS01770 (ACN9KP_01770) nucA/comI 325116..325553 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACN9KP_RS01775 (ACN9KP_01775) hxlB 325912..326469 (-) 558 WP_003156587.1 6-phospho-3-hexuloisomerase -
  ACN9KP_RS01780 (ACN9KP_01780) hxlA 326466..327101 (-) 636 WP_024084873.1 3-hexulose-6-phosphate synthase -
  ACN9KP_RS01785 (ACN9KP_01785) - 327333..327695 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1107282 ACN9KP_RS01770 WP_003156588.1 325116..325553(-) (nucA/comI) [Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1107282 ACN9KP_RS01770 WP_003156588.1 325116..325553(-) (nucA/comI) [Bacillus velezensis strain Ya-1 isolate Tropical rainforest soil]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment