Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACLKM0_RS11130 Genome accession   NZ_CP183907
Coordinates   2310408..2311205 (-) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain M3_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2305408..2316205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM0_RS11110 - 2306451..2307155 (-) 705 WP_005182401.1 YfgM family protein -
  ACLKM0_RS11115 hisS 2307186..2308481 (-) 1296 WP_005182398.1 histidine--tRNA ligase -
  ACLKM0_RS11120 ispG 2308485..2309606 (-) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACLKM0_RS11125 - 2309629..2310417 (-) 789 WP_045795990.1 helix-turn-helix domain-containing protein -
  ACLKM0_RS11130 pilF 2310408..2311205 (-) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACLKM0_RS11135 rlmN 2311241..2312476 (-) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACLKM0_RS11140 ndk 2312599..2313030 (-) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  ACLKM0_RS11145 iscX 2313140..2313340 (-) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  ACLKM0_RS11150 - 2313435..2313986 (-) 552 WP_104470590.1 phosphatase PAP2 family protein -
  ACLKM0_RS11155 - 2314019..2315302 (-) 1284 WP_411687597.1 glycosyltransferase family 4 protein -
  ACLKM0_RS11160 - 2315523..2315948 (-) 426 WP_104470588.1 hypothetical protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=1107132 ACLKM0_RS11130 WP_104470591.1 2310408..2311205(-) (pilF) [Acinetobacter indicus strain M3_NDM]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=1107132 ACLKM0_RS11130 WP_104470591.1 2310408..2311205(-) (pilF) [Acinetobacter indicus strain M3_NDM]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCACTGGAAACAGATTCGCGTGATTCCATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGTAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTCAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCCCTGCAGGCAAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCCGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTAGGACAAAAAAATCAAGGTG
CGCGTGCCTTATGGATTGGGATCCGTGTTGCACGTGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732

Multiple sequence alignment