Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACLKM1_RS02190 Genome accession   NZ_CP183903
Coordinates   474291..475091 (+) Length   266 a.a.
NCBI ID   WP_005172689.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain M5_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469291..480091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM1_RS02160 - 469910..470143 (+) 234 WP_159516235.1 hypothetical protein -
  ACLKM1_RS02165 - 470235..471506 (+) 1272 WP_411689382.1 glycosyltransferase family 4 protein -
  ACLKM1_RS02170 - 471537..472088 (+) 552 WP_005095663.1 phosphatase PAP2 family protein -
  ACLKM1_RS02175 iscX 472176..472376 (+) 201 WP_005172695.1 Fe-S cluster assembly protein IscX -
  ACLKM1_RS02180 ndk 472487..472918 (+) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  ACLKM1_RS02185 rlmN 473041..474276 (+) 1236 WP_005095669.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACLKM1_RS02190 pilF 474291..475091 (+) 801 WP_005172689.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACLKM1_RS02195 - 475082..475873 (+) 792 WP_074384029.1 helix-turn-helix domain-containing protein -
  ACLKM1_RS02200 ispG 475897..477018 (+) 1122 WP_005172685.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACLKM1_RS02205 hisS 477022..478317 (+) 1296 WP_005095678.1 histidine--tRNA ligase -
  ACLKM1_RS02210 - 478348..479043 (+) 696 WP_005095680.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30021.09 Da        Isoelectric Point: 9.0728

>NTDB_id=1107077 ACLKM1_RS02190 WP_005172689.1 474291..475091(+) (pilF) [Acinetobacter pseudolwoffii strain M5_NDM]
MRNLTSKIALISTVCVSLALVACQTPDLGQKDPEKATKVRTQLAAEYLRTGDLDAAKRALDQALETTPRDSQANMMMGVL
LQQEGSQLNLEKADAYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFNIAGATLGYDQRFRALENMGRIYLKMGDV
VNAEKSFKQALQANRDSYISMLELSEIFYLNQQFPAATQLYQQFARGVGQKNQGARALWVGIRTARASGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1107077 ACLKM1_RS02190 WP_005172689.1 474291..475091(+) (pilF) [Acinetobacter pseudolwoffii strain M5_NDM]
TTGAGAAATCTAACATCTAAAATTGCTTTGATTTCAACAGTATGTGTGTCTTTAGCTTTGGTTGCATGTCAAACGCCAGA
TTTAGGTCAGAAAGATCCTGAAAAAGCCACCAAAGTCCGTACCCAATTGGCGGCAGAATATTTAAGAACAGGTGACCTAG
ATGCAGCCAAACGTGCGCTGGATCAGGCACTCGAAACGACTCCGCGTGATTCGCAAGCGAATATGATGATGGGCGTGCTA
TTGCAACAGGAAGGTAGTCAGCTCAATCTTGAGAAAGCAGATGCTTATTTCAAGCGTGCAATTTCGGCAGATCCGAAAAA
TGCACAGGCACGCAACAACTATGGCACGTATTTATATCAGCTAGAACGCTATAATGATGCGATCGAGCAGTTTAACATTG
CCGGTGCAACTTTAGGTTATGATCAGCGTTTCCGTGCGCTGGAAAATATGGGACGTATTTACCTGAAAATGGGCGATGTG
GTGAATGCTGAAAAATCATTCAAACAGGCGCTTCAGGCGAATCGCGATTCATATATATCAATGTTAGAGTTGTCAGAAAT
TTTTTATTTAAACCAGCAGTTCCCGGCAGCAACACAACTGTATCAACAGTTTGCGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTTGGCATTCGCACTGCGCGTGCCAGTGGTGATCAGTTGGGGATGCAGGTATTGGTGAACCAG
TTACGTGCATTGTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_16)

(150-204)

Tetratricopeptide repeat (TPR_14)

(40-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.538

97.744

0.748
  pilF Acinetobacter baylyi ADP1

69.804

95.865

0.669

Multiple sequence alignment