Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACLKM2_RS02185 Genome accession   NZ_CP183899
Coordinates   475043..475843 (+) Length   266 a.a.
NCBI ID   WP_005172689.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470043..480843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM2_RS02155 - 470662..470895 (+) 234 WP_253134160.1 hypothetical protein -
  ACLKM2_RS02160 - 470987..472258 (+) 1272 WP_005172698.1 glycosyltransferase family 4 protein -
  ACLKM2_RS02165 - 472289..472840 (+) 552 WP_005095663.1 phosphatase PAP2 family protein -
  ACLKM2_RS02170 iscX 472928..473128 (+) 201 WP_100329232.1 Fe-S cluster assembly protein IscX -
  ACLKM2_RS02175 ndk 473239..473670 (+) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  ACLKM2_RS02180 rlmN 473793..475028 (+) 1236 WP_411685958.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACLKM2_RS02185 pilF 475043..475843 (+) 801 WP_005172689.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACLKM2_RS02190 - 475834..476625 (+) 792 WP_074384029.1 helix-turn-helix domain-containing protein -
  ACLKM2_RS02195 ispG 476649..477770 (+) 1122 WP_005172685.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACLKM2_RS02200 hisS 477774..479069 (+) 1296 WP_005095678.1 histidine--tRNA ligase -
  ACLKM2_RS02205 - 479100..479795 (+) 696 WP_005095680.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30021.09 Da        Isoelectric Point: 9.0728

>NTDB_id=1107039 ACLKM2_RS02185 WP_005172689.1 475043..475843(+) (pilF) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MRNLTSKIALISTVCVSLALVACQTPDLGQKDPEKATKVRTQLAAEYLRTGDLDAAKRALDQALETTPRDSQANMMMGVL
LQQEGSQLNLEKADAYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFNIAGATLGYDQRFRALENMGRIYLKMGDV
VNAEKSFKQALQANRDSYISMLELSEIFYLNQQFPAATQLYQQFARGVGQKNQGARALWVGIRTARASGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1107039 ACLKM2_RS02185 WP_005172689.1 475043..475843(+) (pilF) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
TTGAGAAATCTAACATCTAAAATTGCTTTGATTTCAACAGTATGTGTGTCTTTAGCTTTGGTTGCATGTCAAACGCCAGA
TTTAGGTCAGAAAGATCCTGAAAAAGCCACCAAAGTCCGTACCCAATTGGCGGCAGAATATTTAAGAACAGGTGACCTAG
ATGCAGCCAAACGTGCGCTGGATCAGGCACTCGAAACGACTCCGCGTGATTCGCAAGCGAATATGATGATGGGCGTGCTA
TTGCAACAGGAAGGTAGTCAGCTCAATCTTGAGAAAGCAGATGCTTATTTCAAGCGTGCAATTTCGGCAGATCCGAAAAA
TGCACAGGCACGCAACAACTATGGCACGTATTTATATCAGCTAGAACGCTATAATGATGCGATCGAGCAGTTTAACATTG
CCGGTGCAACTTTAGGTTATGATCAGCGTTTCCGTGCGCTGGAAAATATGGGACGTATTTACCTGAAAATGGGCGATGTG
GTGAATGCTGAAAAATCATTCAAACAGGCGCTTCAGGCGAATCGCGATTCATATATATCAATGTTAGAGTTGTCAGAAAT
TTTTTATTTAAACCAGCAGTTCCCGGCAGCAACACAACTGTATCAACAGTTTGCGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTTGGCATTCGCACTGCGCGTGCCAGTGGTGATCAGTTGGGGATGCAGGTATTGGTGAACCAG
TTACGTGCATTGTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_16)

(150-204)

Tetratricopeptide repeat (TPR_14)

(40-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.538

97.744

0.748
  pilF Acinetobacter baylyi ADP1

69.804

95.865

0.669

Multiple sequence alignment