Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACLKM2_RS01335 Genome accession   NZ_CP183899
Coordinates   291620..293842 (-) Length   740 a.a.
NCBI ID   WP_180086553.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 286620..298842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM2_RS01310 - 286836..287099 (-) 264 WP_005095339.1 YdcH family protein -
  ACLKM2_RS01315 - 287188..287520 (-) 333 WP_005095341.1 chaperone modulator CbpM -
  ACLKM2_RS01320 - 287980..288441 (-) 462 WP_005095344.1 DUF2147 domain-containing protein -
  ACLKM2_RS01325 - 288765..289640 (-) 876 WP_005095347.1 Hsp33 family molecular chaperone HslO -
  ACLKM2_RS01330 - 289691..291544 (-) 1854 WP_411685985.1 cation:proton antiporter -
  ACLKM2_RS01335 priA 291620..293842 (-) 2223 WP_180086553.1 primosomal protein N' Machinery gene
  ACLKM2_RS01340 gspF 293993..295198 (+) 1206 WP_074383949.1 type II secretion system inner membrane protein GspF -
  ACLKM2_RS01345 gspG 295278..295712 (+) 435 WP_005095357.1 type II secretion system major pseudopilin GspG -
  ACLKM2_RS01350 - 295997..296332 (+) 336 WP_100355491.1 hypothetical protein -
  ACLKM2_RS01355 lnt 296486..298045 (-) 1560 WP_100329308.1 apolipoprotein N-acyltransferase -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 84154.14 Da        Isoelectric Point: 8.5060

>NTDB_id=1107035 ACLKM2_RS01335 WP_180086553.1 291620..293842(-) (priA) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
MQNSHTDSSPLCRVRVAVPVHVFDCFDYSMNAEQFEQAQVGARVLVSFGRQNLVGVIIEKLSADTPVDPRFKLKPVTELL
DERAIIDPKVLSLLTWSAQYYQFPMGEVVHSALPTLLRQGKPYNLLARTWKLLNPDAEAKIRRSEKQQEAYRILKLHPVG
TTENVLNLAGIETATLKALEKKEICTCVLEPQDFSPQPMQLAQMPLTANPEQKHAIDQVLKYRNQYQAFLLDGLTGSGKT
EVYLQIMQQVLKQGKQVLVLVPEIGLTPQTISRFQSRFQCHIALLHSGLNDTKRLQAWQAAETGKASIILGTRSAIYTPI
PNLGLIILDEEHDLSFKQQEGFRYHARDVALYRGHLQQCPVLLGSATPSIDSYALVEHGKMQVLELNQRAGTALMPKIHI
LDLKVAQKKHGISLQLIQDIKKRLEKKEQVLIFLNRRGYAPVLLCGSCGWQAQCPHCDANFTVHRQPYQHLHCHHCGTIH
RMPEYCPQCQHQELVTLGMGTGKVEEHLNELFPDHEVIRVDRDSTSRVGSWQKIYDKIQKSEPAILLGTQMLAKGHHFPY
VTLVAILDIDSGLLSVDFRATERTAQLIVQVAGRAGRGEHKGDVYLQTLRPDHALLNTLVNENYRVFAQQTLKERQIAQM
PPYRYAILIRCESKDQAQNTEFLQKHAAFLRQYPDLALDIWGPIPAPMERKAGRYQSHMVLLSADRPRLHYYVRSWWQNM
LQDKPSSMKLTLDIDPQELS

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=1107035 ACLKM2_RS01335 WP_180086553.1 291620..293842(-) (priA) [Acinetobacter pseudolwoffii strain M8_NDM_tet(X3)]
ATGCAAAATTCTCATACCGATTCTAGTCCACTTTGTCGCGTACGCGTTGCTGTACCTGTGCATGTATTCGATTGTTTTGA
TTACAGTATGAATGCAGAACAATTTGAGCAGGCCCAGGTCGGCGCACGCGTATTGGTATCTTTTGGACGACAGAATCTGG
TTGGGGTGATTATCGAAAAGCTCTCCGCAGATACTCCGGTCGACCCACGTTTTAAACTCAAGCCCGTCACTGAATTGCTG
GATGAACGTGCCATCATAGACCCTAAAGTTTTAAGTTTGTTGACATGGTCGGCACAGTATTATCAGTTTCCGATGGGTGA
AGTGGTGCACAGTGCCCTGCCAACGCTGTTACGCCAAGGTAAGCCTTATAACCTGCTGGCCCGCACCTGGAAACTTCTGA
ACCCTGATGCTGAAGCTAAAATACGCCGTTCGGAAAAGCAGCAGGAAGCCTACCGGATTTTAAAACTGCATCCGGTTGGC
ACCACTGAAAATGTATTGAATTTGGCCGGAATTGAAACTGCGACCTTAAAAGCACTGGAGAAAAAAGAGATTTGTACCTG
TGTGCTGGAGCCACAAGATTTCAGTCCACAGCCGATGCAATTGGCGCAAATGCCGCTAACCGCTAATCCTGAACAGAAAC
ATGCCATTGATCAGGTATTAAAGTATCGCAATCAATATCAGGCTTTTTTACTGGATGGTCTGACCGGCAGTGGTAAAACC
GAAGTCTATCTACAAATCATGCAACAGGTGCTGAAACAGGGCAAGCAGGTTTTGGTGCTGGTGCCGGAAATTGGTCTGAC
CCCGCAAACGATTAGCCGTTTCCAGTCGCGTTTTCAGTGTCATATCGCGCTATTGCATTCAGGCCTAAACGATACAAAAC
GTCTGCAAGCCTGGCAGGCAGCAGAGACTGGCAAAGCTTCAATCATCCTCGGCACACGTTCCGCCATCTATACCCCCATA
CCGAATTTGGGTCTGATCATTCTGGATGAAGAACATGATTTGTCTTTTAAGCAGCAGGAAGGTTTCCGCTATCATGCCCG
CGATGTGGCGCTGTACCGCGGCCATTTACAGCAATGTCCAGTGCTGCTGGGATCAGCGACACCAAGTATCGACAGTTATG
CGCTGGTTGAACATGGCAAAATGCAGGTGCTGGAGCTGAATCAACGTGCCGGCACAGCCTTAATGCCGAAAATCCATATT
CTGGATTTAAAGGTGGCACAGAAAAAGCATGGCATCAGTCTGCAACTGATTCAGGACATCAAAAAGCGTCTGGAGAAAAA
AGAACAGGTACTGATCTTTTTAAACCGCCGTGGCTATGCCCCCGTCCTGCTTTGTGGCAGTTGTGGCTGGCAGGCACAAT
GTCCGCACTGCGATGCCAATTTTACTGTGCATCGCCAGCCTTATCAGCATCTGCATTGCCATCACTGTGGCACCATTCAC
CGAATGCCGGAGTACTGTCCGCAGTGTCAGCATCAGGAACTGGTCACACTGGGTATGGGCACTGGCAAGGTCGAAGAACA
TCTGAATGAACTTTTTCCAGATCATGAAGTGATTCGGGTCGATCGCGATTCCACCAGCCGGGTGGGTAGCTGGCAAAAAA
TCTATGACAAGATTCAAAAAAGTGAACCCGCGATTCTGCTCGGCACCCAGATGCTGGCCAAAGGGCATCATTTTCCTTAT
GTGACGCTGGTGGCGATTCTGGATATTGATTCCGGTCTGCTCAGTGTGGATTTCCGTGCCACCGAACGTACCGCACAACT
GATCGTTCAGGTCGCAGGCCGGGCTGGACGTGGTGAACACAAAGGCGATGTCTATTTGCAGACGCTCAGACCGGATCATG
CCCTGCTGAATACCTTGGTCAATGAAAATTATCGTGTGTTTGCCCAGCAGACCTTAAAGGAACGGCAAATTGCCCAGATG
CCGCCTTATCGTTATGCCATCCTGATTCGCTGCGAATCCAAGGATCAGGCACAAAACACCGAATTCTTACAGAAACATGC
GGCATTCCTCAGGCAATATCCTGATCTGGCGCTGGACATCTGGGGGCCAATTCCTGCGCCGATGGAACGTAAGGCTGGGC
GCTATCAGTCGCATATGGTGCTCTTGTCAGCAGATCGTCCACGTTTGCATTACTATGTGCGCAGCTGGTGGCAGAATATG
CTGCAGGACAAACCTTCCAGCATGAAGCTGACACTGGACATTGACCCGCAAGAACTCAGCTAA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(15-114)

Primosomal protein N C-terminal domain (PriA_C)

(642-736)

Type III restriction enzyme, res subunit (ResIII)

(209-369)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

71.117

99.189

0.705

Multiple sequence alignment