Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACLKM3_RS02755 Genome accession   NZ_CP183897
Coordinates   587962..588771 (+) Length   269 a.a.
NCBI ID   WP_019836295.1    Uniprot ID   -
Organism   Acinetobacter towneri strain M10_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 582962..593771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM3_RS02725 - 583136..583561 (+) 426 WP_236162616.1 hypothetical protein -
  ACLKM3_RS02730 - 583781..585064 (+) 1284 WP_411690676.1 glycosyltransferase family 4 protein -
  ACLKM3_RS02735 - 585090..585641 (+) 552 WP_019836292.1 phosphatase PAP2 family protein -
  ACLKM3_RS02740 iscX 585743..585943 (+) 201 WP_019836293.1 Fe-S cluster assembly protein IscX -
  ACLKM3_RS02745 ndk 586105..586536 (+) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  ACLKM3_RS02750 rlmN 586675..587907 (+) 1233 WP_019836294.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACLKM3_RS02755 pilF 587962..588771 (+) 810 WP_019836295.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACLKM3_RS02760 - 588762..589571 (+) 810 WP_411690677.1 helix-turn-helix domain-containing protein -
  ACLKM3_RS02765 ispG 589594..590715 (+) 1122 WP_411690678.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACLKM3_RS02770 hisS 590719..592014 (+) 1296 WP_236162619.1 histidine--tRNA ligase -
  ACLKM3_RS02775 - 592045..592746 (+) 702 WP_019836299.1 YfgM family protein -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 30240.28 Da        Isoelectric Point: 9.2875

>NTDB_id=1107006 ACLKM3_RS02755 WP_019836295.1 587962..588771(+) (pilF) [Acinetobacter towneri strain M10_NDM]
MNKPVFKFAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSSANMMM
GVLLQQEGSKLNLEKADAYFKRAIAADPQNAQARNNYGTYLYQVERYNEAIEQLSIAGATLGYDQRFRALENVGRIYLKL
GDVANAEKSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVL
VNQLRALFPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 810 bp        

>NTDB_id=1107006 ACLKM3_RS02755 WP_019836295.1 587962..588771(+) (pilF) [Acinetobacter towneri strain M10_NDM]
TTGAATAAGCCTGTATTTAAATTCGCACAAATTACAACCGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCAC
AGCGCCAACGCTCGGACAAAAAGACCCAGAAAAAGCAGTACAAGTACGCACTCAACTTGCCGCAGAATATTTAAAAAATG
GCGACTTAGATTCTGCTAAGCGTGCGTTGGATCAAGCTTTGGAAAGCGATTCACGCGATTCATCTGCCAATATGATGATG
GGAGTGTTGCTGCAACAAGAAGGCAGCAAGCTGAATCTAGAAAAAGCCGATGCCTATTTCAAGCGTGCCATTGCTGCTGA
CCCTCAAAATGCACAAGCGCGTAACAACTATGGTACGTATTTATATCAAGTAGAACGTTATAATGAGGCGATTGAACAGC
TCAGTATTGCTGGTGCAACACTGGGTTATGATCAGCGTTTTAGAGCATTAGAAAACGTAGGGCGTATTTATTTAAAGTTG
GGTGATGTCGCGAATGCCGAAAAATCATTCAAACAAGCCTTGCAAGCCAACCGCGATTCTTATGTTTCAATGTTAGAGTT
GTCTGAAATTTTATATTTAAGACAACAATTTGCACCCGCTCGACAATTGTATGAACAGTTTGTACGTGCGGTCGGGCAAA
AAAATCAGGGTGCGCGTGCCTTATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTA
GTGAACCAGCTGCGTGCATTATTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGT
ATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(92-134)

Tetratricopeptide repeat (TPR_16)

(46-80)

Tetratricopeptide repeat (TPR_16)

(154-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

73.606

100

0.736
  pilF Acinetobacter baylyi ADP1

72.835

94.424

0.688

Multiple sequence alignment