Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACLKM4_RS02640 Genome accession   NZ_CP183895
Coordinates   556076..556873 (+) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain D1_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551076..561873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM4_RS02610 - 551333..551758 (+) 426 WP_104470588.1 hypothetical protein -
  ACLKM4_RS02615 - 551979..553262 (+) 1284 WP_411687597.1 glycosyltransferase family 4 protein -
  ACLKM4_RS02620 - 553295..553846 (+) 552 WP_104470590.1 phosphatase PAP2 family protein -
  ACLKM4_RS02625 iscX 553941..554141 (+) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  ACLKM4_RS02630 ndk 554251..554682 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  ACLKM4_RS02635 rlmN 554805..556040 (+) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACLKM4_RS02640 pilF 556076..556873 (+) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACLKM4_RS02645 - 556864..557652 (+) 789 WP_016659532.1 helix-turn-helix domain-containing protein -
  ACLKM4_RS02650 ispG 557675..558796 (+) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACLKM4_RS02655 hisS 558800..560095 (+) 1296 WP_411687598.1 histidine--tRNA ligase -
  ACLKM4_RS02660 - 560126..560830 (+) 705 WP_005182401.1 YfgM family protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=1106967 ACLKM4_RS02640 WP_104470591.1 556076..556873(+) (pilF) [Acinetobacter indicus strain D1_NDM_tet(X3)]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=1106967 ACLKM4_RS02640 WP_104470591.1 556076..556873(+) (pilF) [Acinetobacter indicus strain D1_NDM_tet(X3)]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCACTGGAAACAGATTCGCGTGATTCCATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGCAGTAAGCTGAATCTGGAGAAGGCCGATACGTATTTTAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCTCTGCAGGCGAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCAGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCATTGTGGATTGGGATCCGTGTTGCACGAGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732

Multiple sequence alignment