Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACLKM6_RS02490 Genome accession   NZ_CP183890
Coordinates   527169..527966 (+) Length   265 a.a.
NCBI ID   WP_104470591.1    Uniprot ID   -
Organism   Acinetobacter indicus strain D11_NDM_tet(X5)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 522169..532966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM6_RS02460 - 522485..522904 (+) 420 WP_411687097.1 hypothetical protein -
  ACLKM6_RS02465 - 523072..524355 (+) 1284 WP_168397903.1 glycosyltransferase family 4 protein -
  ACLKM6_RS02470 - 524388..524939 (+) 552 WP_104470590.1 phosphatase PAP2 family protein -
  ACLKM6_RS02475 iscX 525034..525234 (+) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  ACLKM6_RS02480 ndk 525344..525775 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  ACLKM6_RS02485 rlmN 525898..527133 (+) 1236 WP_411687098.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACLKM6_RS02490 pilF 527169..527966 (+) 798 WP_104470591.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACLKM6_RS02495 - 527957..528745 (+) 789 WP_045795990.1 helix-turn-helix domain-containing protein -
  ACLKM6_RS02500 ispG 528768..529889 (+) 1122 WP_411687099.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACLKM6_RS02505 hisS 529893..531188 (+) 1296 WP_411687100.1 histidine--tRNA ligase -
  ACLKM6_RS02510 - 531219..531923 (+) 705 WP_005182401.1 YfgM family protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29946.85 Da        Isoelectric Point: 8.3587

>NTDB_id=1106884 ACLKM6_RS02490 WP_104470591.1 527169..527966(+) (pilF) [Acinetobacter indicus strain D11_NDM_tet(X5)]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKSFKQALQANRDSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=1106884 ACLKM6_RS02490 WP_104470591.1 527169..527966(+) (pilF) [Acinetobacter indicus strain D11_NDM_tet(X5)]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTGGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCGGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCACTGGAAACAGATTCGCGTGATTCTATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGTAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTCAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAACAGCTGAGTATTGCCG
GGGCAACACTTGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCG
AATGCTGAAAAATCATTCAAGCAGGCTCTGCAGGCGAACCGTGACTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCAGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTGGGACAAAAAAATCAAGGTG
CGCGTGCATTGTGGATTGGGATCCGTGTTGCACGAGCAAATGGCGATCAGTTGGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-175)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.992

97.736

0.762
  pilF Acinetobacter baylyi ADP1

72.119

100

0.732

Multiple sequence alignment