Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACN222_RS12215 Genome accession   NZ_CP183473
Coordinates   2934617..2936629 (-) Length   670 a.a.
NCBI ID   WP_010996600.1    Uniprot ID   Q8YUA8
Organism   Anabaena sp. FACHB-595     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2929617..2941629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN222_RS12195 (ACN222_12225) bioU 2930209..2931213 (+) 1005 WP_010996596.1 (S)-8-amino-7-oxononanoate synthase BioU -
  ACN222_RS12200 (ACN222_12230) - 2931281..2931835 (-) 555 WP_010996597.1 hypothetical protein -
  ACN222_RS12205 (ACN222_12235) - 2932119..2933343 (-) 1225 Protein_2409 type II secretion system F family protein -
  ACN222_RS12210 (ACN222_12240) pilT 2933451..2934569 (-) 1119 WP_010996599.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN222_RS12215 (ACN222_12245) pilB/pilB1 2934617..2936629 (-) 2013 WP_010996600.1 GspE/PulE family protein Machinery gene
  ACN222_RS12220 (ACN222_12250) grpE 2937015..2937761 (+) 747 WP_010996601.1 nucleotide exchange factor GrpE -
  ACN222_RS12225 (ACN222_12255) dnaK 2938044..2940005 (+) 1962 WP_010996602.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75006.90 Da        Isoelectric Point: 5.1212

>NTDB_id=1105844 ACN222_RS12215 WP_010996600.1 2934617..2936629(-) (pilB/pilB1) [Anabaena sp. FACHB-595]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYINTEQMRQALIESRKSGRPLTEVLESITGRQLSPEYLRQYKKQQLFELK
ILYGVEYLDPEVNNIGSSMVGNLIETLIPVDICRRHRLVPLSKNDEHTPPYVLVAMVAPDNLEASDDLNRILRPQGLTLQ
RMVITQEDYQQLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDLEDAPEEMEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLQIVQDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIAYTPTTEELARYGMSASQEVSVTFYKANIVPAEAIAEAKAKNELCPACNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KTGLTCRTCSAILQPEWLDCPYCLTPRFED

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=1105844 ACN222_RS12215 WP_010996600.1 2934617..2936629(-) (pilB/pilB1) [Anabaena sp. FACHB-595]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTTACTACAAGAACAGAGTTTTCGCCTTTCGGTAATAAGCTAGT
GCAGTCTGGCTACATTAATACCGAACAGATGAGACAGGCGCTGATTGAAAGTCGCAAGTCTGGTAGACCCTTAACAGAAG
TTTTGGAGTCAATTACTGGGCGACAATTGTCACCTGAGTATCTCAGGCAGTACAAGAAACAACAATTATTTGAACTGAAA
ATACTATACGGTGTTGAATACCTCGATCCGGAGGTCAACAATATCGGTAGCTCGATGGTGGGTAATTTAATTGAAACCCT
CATCCCAGTTGATATCTGCCGTCGTCATCGTTTAGTTCCACTATCTAAAAATGATGAACATACGCCGCCTTATGTTTTAG
TGGCGATGGTGGCTCCCGATAACTTGGAAGCATCGGATGACCTAAACCGCATCTTACGCCCCCAAGGTTTAACATTACAG
CGTATGGTAATTACCCAGGAGGATTACCAACAGCTAATTAACCAATATTTGGATGAATTAGCTGTGCGGCAAAAGCACCT
GGAACAAGAAAAATTTACAGATATTAATCAGGATTTAGAAAATCTTGGCAATCTCGACCTAGAAGATGCTCCTGAAGAGA
TGGAGGCTGATTTAGGTTCAGCAATGAAGGGTGCAGAGGATGCACCAGTCATCAACCTCGTCAATAGAATTTTGGCGAAG
GCTTTGCATGAGAAGGTTTCTGATATCCACATCGAACCCCAAGAAGAAAACCTGCGGATTCGCTTCCGCAAGGATGGGGT
ACTGCGGGAGGCTTTTGACCCCCTACCGAAAAAAATTATTCCGGCTGTTACAGCTAGATTTAAAATTATCTCCAATTTAG
ATATTGCCGAGCGACGTTTACCCCAAGATGGACGTATCCGGCGGATGTTTGAGGGACGCAAGGTTGACTTCCGGGTGAAT
ACCTTACCTAGTCGCTATGGGGAAAAGGTAGTACTACGAATTTTGGATAATTCTTCTACCCAACTGGGGCTAAATAAGTT
AATCACTGACCCAGAAACATTGCAGATTGTCCAAGATATGGTGAGTAAGCCTTTCGGGTTGATTTTGGTGACTGGGCCGA
CTGGTTCAGGTAAAACCACTTCCTTGTATTCGGCACTGTCTGAGAAGAACTCACCAGGTATTAATATCAGTACGGTAGAA
GACCCGATTGAGTACAGTTTGCCGGGGATTACTCAAGTACAGGTAATTCGGGAAAAAGGGCTAGATTTTGCCACAGCATT
ACGGGCTTTTTTGCGACAAGATCCTGATGTGTTGCTGGTGGGTGAAACAAGAGACAAAGAAACAGCGAAAACAGCGATTG
AAGCGGCTTTAACAGGTCACTTGGTCTTAACTACATTACACACCAACGATGCCCCTGGTGCGATCGCTCGTTTGGGCGAA
ATGGGTATTGAACCATTCATGGTTTCCAGTTCTCTAATTGGGGTATTGGCACAACGTCTAGTGCGCCGTGTTTGTAGTGA
GTGCCGTATTGCTTATACTCCCACCACTGAGGAATTAGCTCGTTATGGAATGTCTGCTTCTCAAGAAGTGTCTGTCACCT
TCTATAAAGCTAACATTGTCCCAGCAGAAGCGATCGCCGAAGCCAAAGCTAAAAATGAGCTTTGTCCAGCTTGTAACGGT
GTCGGCTATAAAGGACGCTGTGGTGTTTATGAAGTGATGCGGGTTACAGAAAACCTACAAACTTTAATCAACGAAGAAGC
ACCAACAGAACGCATCAAAGAAGTAGCCGTAGAAGAAGGGATGAAAACCTTATTGGCTTATAGCCTAGACCTCGTTCGCC
AAGGTGCTACCACCTTAGAAGAAGTGGAACGGGTGACATTCACAGACACAGGTTTGGAAGCTGAGTTAAAAGCTAAACGC
AAGACTGGTCTTACCTGCCGGACGTGTAGTGCCATATTACAACCAGAATGGCTAGATTGTCCCTACTGTCTAACACCCCG
TTTTGAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8YUA8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.179

100

0.654
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.74

94.776

0.367

Multiple sequence alignment