Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACN22W_RS15535 Genome accession   NZ_CP183461
Coordinates   3294611..3296116 (+) Length   501 a.a.
NCBI ID   WP_129512998.1    Uniprot ID   -
Organism   Burkholderia theae strain XITOU     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3289611..3301116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN22W_RS15510 (ACN22W_15510) kdsD 3290854..3291837 (+) 984 WP_129512993.1 arabinose 5-phosphate isomerase KdsD -
  ACN22W_RS15515 (ACN22W_15515) - 3291837..3292373 (+) 537 WP_129512994.1 KdsC family phosphatase -
  ACN22W_RS15520 (ACN22W_15520) lptC 3292377..3292991 (+) 615 WP_419685341.1 LPS export ABC transporter periplasmic protein LptC -
  ACN22W_RS15525 (ACN22W_15525) lptA 3293012..3293677 (+) 666 WP_419685342.1 lipopolysaccharide transport periplasmic protein LptA -
  ACN22W_RS15530 (ACN22W_15530) lptB 3293674..3294450 (+) 777 WP_129512997.1 LPS export ABC transporter ATP-binding protein -
  ACN22W_RS15535 (ACN22W_15535) rpoN/rpoN1 3294611..3296116 (+) 1506 WP_129512998.1 RNA polymerase factor sigma-54 Machinery gene
  ACN22W_RS15540 (ACN22W_15540) hpf 3296275..3296634 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACN22W_RS15545 (ACN22W_15545) - 3296833..3297345 (+) 513 WP_048246965.1 PTS sugar transporter subunit IIA -
  ACN22W_RS15550 (ACN22W_15550) hprK 3297475..3298443 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  ACN22W_RS15555 (ACN22W_15555) rapZ 3298519..3299427 (+) 909 WP_419685343.1 RNase adapter RapZ -
  ACN22W_RS15560 (ACN22W_15560) - 3299455..3300090 (+) 636 WP_048246968.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54823.90 Da        Isoelectric Point: 4.8740

>NTDB_id=1105783 ACN22W_RS15535 WP_129512998.1 3294611..3296116(+) (rpoN/rpoN1) [Burkholderia theae strain XITOU]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVASDGSLIAQTPPAQNTEPSA
ANSSSQSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPSELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIMVKKVGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1105783 ACN22W_RS15535 WP_129512998.1 3294611..3296116(+) (rpoN/rpoN1) [Burkholderia theae strain XITOU]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTCCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGTTGCGCGTTGCATCGGACGGATCGCTGATCGCGCAGACGCCCCCCGCGCAGAACACCGAGCCAAGCGCG
GCGAACAGCAGCAGCCAGTCGAGCGATCGCGCGGAGCGCGACGAACCGGCCGGCGCGGACGAATACGACGGTTATGGATC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATACGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTACAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CGCCGACCTGCCGTCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGCGCCCGCTCGGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTGGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCTGACATCATGGTGAAGAAGGTCGGCCAGGGATGGCTCGCGGAACTCAATCCGGAGGTCGTGCCG
CGTCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAAAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.984

100

0.687

Multiple sequence alignment