Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACN262_RS33555 Genome accession   NZ_CP183460
Coordinates   3796124..3797629 (+) Length   501 a.a.
NCBI ID   WP_036049140.1    Uniprot ID   A0AAW7R6G5
Organism   Burkholderia gladioli strain BG-GF5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3791124..3802629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN262_RS33530 (ACN262_33525) kdsD 3792333..3793316 (+) 984 WP_025096995.1 arabinose 5-phosphate isomerase KdsD -
  ACN262_RS33535 (ACN262_33530) - 3793316..3793855 (+) 540 WP_186119289.1 KdsC family phosphatase -
  ACN262_RS33540 (ACN262_33535) lptC 3793859..3794473 (+) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  ACN262_RS33545 (ACN262_33540) lptA 3794492..3795160 (+) 669 WP_036049142.1 lipopolysaccharide transport periplasmic protein LptA -
  ACN262_RS33550 (ACN262_33545) lptB 3795157..3795933 (+) 777 WP_013699406.1 LPS export ABC transporter ATP-binding protein -
  ACN262_RS33555 (ACN262_33550) rpoN/rpoN1 3796124..3797629 (+) 1506 WP_036049140.1 RNA polymerase factor sigma-54 Machinery gene
  ACN262_RS33560 (ACN262_33555) hpf 3797780..3798145 (+) 366 WP_013699408.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACN262_RS33565 (ACN262_33560) - 3798359..3798871 (+) 513 WP_025097001.1 PTS sugar transporter subunit IIA -
  ACN262_RS33570 (ACN262_33565) hprK 3798997..3799965 (+) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  ACN262_RS33575 (ACN262_33570) rapZ 3800076..3800969 (+) 894 WP_013699411.1 RNase adapter RapZ -
  ACN262_RS33580 (ACN262_33575) - 3801002..3801637 (+) 636 WP_036049137.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55077.04 Da        Isoelectric Point: 4.9483

>NTDB_id=1105769 ACN262_RS33555 WP_036049140.1 3796124..3797629(+) (rpoN/rpoN1) [Burkholderia gladioli strain BG-GF5]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEASGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=1105769 ACN262_RS33555 WP_036049140.1 3796124..3797629(+) (rpoN/rpoN1) [Burkholderia gladioli strain BG-GF5]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CGAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCCTCCTCGACGCCCGAGCCGATGCACGGT
GGCGGCTCGAACGGCAGCGAAGCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGTCGCTCCTCGGGCGCCTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCACGAGGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTGACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAAGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGGGCGCGCTCCGCATCGGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCATGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGAGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACGACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCGCACGTGTCGACGGATACCGGGGGTGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment