Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACNSPE_RS23420 Genome accession   NZ_CP183326
Coordinates   4642983..4644362 (-) Length   459 a.a.
NCBI ID   WP_057767155.1    Uniprot ID   A0A4R1AZZ2
Organism   Cytobacillus praedii strain MM2025     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4637983..4649362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNSPE_RS23395 (ACNSPE_23395) ispF 4639400..4639879 (-) 480 WP_328160072.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACNSPE_RS23400 (ACNSPE_23400) - 4639905..4640132 (-) 228 WP_419393146.1 IspD/TarI family cytidylyltransferase -
  ACNSPE_RS23405 (ACNSPE_23405) - 4640087..4640593 (-) 507 WP_419393147.1 IspD/TarI family cytidylyltransferase -
  ACNSPE_RS23410 (ACNSPE_23410) - 4640615..4641697 (-) 1083 WP_057767159.1 PIN/TRAM domain-containing protein -
  ACNSPE_RS23415 (ACNSPE_23415) disA 4641906..4642979 (-) 1074 WP_131237681.1 DNA integrity scanning diadenylate cyclase DisA -
  ACNSPE_RS23420 (ACNSPE_23420) radA 4642983..4644362 (-) 1380 WP_057767155.1 DNA repair protein RadA Machinery gene
  ACNSPE_RS23425 (ACNSPE_23425) clpC 4644752..4647205 (-) 2454 WP_057767154.1 ATP-dependent protease ATP-binding subunit ClpC -
  ACNSPE_RS23430 (ACNSPE_23430) - 4647222..4648289 (-) 1068 WP_057767152.1 protein arginine kinase -
  ACNSPE_RS23435 (ACNSPE_23435) - 4648291..4648839 (-) 549 WP_419393148.1 UvrB/UvrC motif-containing protein -
  ACNSPE_RS23440 (ACNSPE_23440) - 4648865..4649326 (-) 462 WP_057767148.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49818.22 Da        Isoelectric Point: 7.4033

>NTDB_id=1105309 ACNSPE_RS23420 WP_057767155.1 4642983..4644362(-) (radA) [Cytobacillus praedii strain MM2025]
MAKRKTKFMCQECGYESPKWMGKCPGCGEWNKMVEEVEKPAASRRGAFAHSAGSTVLSKATPINSIETVSEPRIYTDLNE
LNRVLGGGVVRGSLVLIGGDPGIGKSTLLLQVSSQLANKNHSVLYISGEESMRQTKLRADRLGVSSDNLLVYSETSLEEI
SRTIDQVNPSFVIIDSIQTIFHPEVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGSIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFRDKPTRPTDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPSNNLGGWKGPKGVELIGVGSVSEALKATLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1105309 ACNSPE_RS23420 WP_057767155.1 4642983..4644362(-) (radA) [Cytobacillus praedii strain MM2025]
ATGGCCAAAAGAAAAACAAAGTTTATGTGCCAGGAATGCGGGTATGAGTCACCAAAGTGGATGGGGAAATGTCCAGGCTG
TGGCGAGTGGAATAAAATGGTCGAAGAAGTAGAGAAGCCTGCTGCCTCAAGAAGGGGAGCATTTGCCCATTCCGCAGGAT
CAACTGTACTCTCAAAGGCGACACCAATTAACTCTATTGAGACAGTAAGTGAGCCAAGGATCTATACAGATTTAAATGAA
CTTAATCGTGTGCTCGGAGGCGGAGTAGTTAGAGGGTCTCTTGTGCTGATCGGCGGTGATCCTGGAATCGGTAAATCTAC
GCTGCTCCTGCAGGTTTCATCTCAACTTGCTAATAAAAACCACTCTGTTTTGTATATATCTGGGGAAGAATCGATGCGTC
AAACAAAATTAAGAGCGGATCGTTTAGGTGTTTCTTCAGATAATTTATTAGTCTACTCGGAGACAAGTCTTGAAGAAATC
AGCAGGACGATCGATCAAGTGAATCCAAGCTTTGTAATTATTGATTCAATTCAAACAATCTTTCATCCAGAGGTTACTTC
AGCACCTGGAAGTGTTTCTCAAGTAAGGGAGTGCACGGCTGAATTAATGAGAATTGGGAAGACAAAAGGAATTGCCATTT
TCATAGTCGGGCATGTTACAAAGGAAGGCTCCATTGCCGGACCAAGATTATTAGAGCATATGGTAGATACTGTGCTTTAT
TTTGAGGGAGAACGCCATCATACATATCGTATTTTGCGAGCGGTAAAAAACAGATTTGGATCTACAAATGAAATGGGAAT
TTTTGAAATGAAGGAGTTTGGACTGGAAGAGGTAGAAAACCCGTCTGAAATATTCCTAGAAGAAAGATCCCAAGGTGCAG
CAGGATCAACTGTTGTTGCATCAATGGAAGGAACACGTCCTGTTTTAGTGGAAATACAGGCGCTTATTTCCCCAACCAGT
TTTGGGAACCCAAGAAGGATGGCAACGGGGATTGATCATAACCGTGTGCCGCTGCTAATGGCTGTACTGGAAAAAAGGGT
TGGGATGCTCTTGCAGAACCAGGATGCTTATTTAAAGGTAGCTGGTGGAGTGAAGCTGGATGAGCCAGCCATCGATTTAG
CCATTGCAGTCAGCATCGCATCCAGTTTTCGTGATAAGCCAACAAGGCCAACTGATTGTATCATTGGAGAAGTCGGATTA
ACTGGTGAAGTTAGAAGGGTATCAAGAATAGAGCAAAGGGTTCAAGAAGCAGCTAAGCTTGGCTTTGAAAGAGTTATTTT
ACCATCTAATAATCTTGGTGGCTGGAAGGGGCCAAAAGGAGTAGAACTAATAGGGGTAGGTTCTGTTAGTGAAGCACTAA
AAGCGACATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R1AZZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.609

100

0.778
  radA Streptococcus mitis NCTC 12261

62.335

98.911

0.617
  radA Streptococcus pneumoniae Rx1

62.335

98.911

0.617
  radA Streptococcus pneumoniae D39

62.335

98.911

0.617
  radA Streptococcus pneumoniae R6

62.335

98.911

0.617
  radA Streptococcus pneumoniae TIGR4

62.335

98.911

0.617
  radA Streptococcus mitis SK321

62.115

98.911

0.614

Multiple sequence alignment