Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACN2AT_RS12830 Genome accession   NZ_CP183238
Coordinates   2497917..2498684 (-) Length   255 a.a.
NCBI ID   WP_003230055.1    Uniprot ID   A0AAE2SJ05
Organism   Bacillus subtilis strain NDmed     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2492917..2503684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN2AT_RS12810 (ACN2AT_12715) ppsR 2493092..2493904 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  ACN2AT_RS12815 (ACN2AT_12720) ccpN 2493930..2494568 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ACN2AT_RS12820 (ACN2AT_12725) glyS 2494701..2496740 (-) 2040 WP_003230059.1 glycine--tRNA ligase subunit beta -
  ACN2AT_RS12825 (ACN2AT_12730) glyQ 2496733..2497620 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ACN2AT_RS12830 (ACN2AT_12735) recO 2497917..2498684 (-) 768 WP_003230055.1 DNA repair protein RecO Machinery gene
  ACN2AT_RS12835 (ACN2AT_12740) yqzL 2498721..2498864 (-) 144 WP_003230053.1 YqzL family protein -
  ACN2AT_RS12840 (ACN2AT_12745) era 2499012..2499917 (-) 906 WP_003230051.1 GTPase Era -
  ACN2AT_RS12845 (ACN2AT_12750) - 2499898..2500308 (-) 411 WP_003230049.1 cytidine deaminase -
  ACN2AT_RS12850 (ACN2AT_12755) unpK 2500427..2500798 (-) 372 WP_004398824.1 diacylglycerol kinase family protein -
  ACN2AT_RS12855 (ACN2AT_12760) ybeY 2500779..2501252 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  ACN2AT_RS12860 (ACN2AT_12765) pgpH 2501253..2503388 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=1104687 ACN2AT_RS12830 WP_003230055.1 2497917..2498684(-) (recO) [Bacillus subtilis strain NDmed]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1104687 ACN2AT_RS12830 WP_003230055.1 2497917..2498684(-) (recO) [Bacillus subtilis strain NDmed]
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment