Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | ACNM7T_RS08955 | Genome accession | NZ_CP183041 |
| Coordinates | 1823209..1823643 (+) | Length | 144 a.a. |
| NCBI ID | WP_015417516.1 | Uniprot ID | - |
| Organism | Bacillus amyloliquefaciens strain Scm | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 1824514..1838371 | 1823209..1823643 | flank | 871 |
| IScluster/Tn | 1824514..1825664 | 1823209..1823643 | flank | 871 |
Gene organization within MGE regions
Location: 1823209..1838371
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACNM7T_RS08955 (ACNM7T_08955) | nucA/comI | 1823209..1823643 (+) | 435 | WP_015417516.1 | NucA/NucB deoxyribonuclease domain-containing protein | Machinery gene |
| ACNM7T_RS08960 (ACNM7T_08960) | - | 1823703..1824458 (+) | 756 | WP_003154084.1 | YoaK family protein | - |
| ACNM7T_RS08970 (ACNM7T_08970) | - | 1825780..1826142 (-) | 363 | WP_007410383.1 | hypothetical protein | - |
| ACNM7T_RS08975 (ACNM7T_08975) | - | 1826335..1827663 (-) | 1329 | WP_039063127.1 | S8 family peptidase | - |
| ACNM7T_RS08980 (ACNM7T_08980) | - | 1827842..1828075 (+) | 234 | WP_015239897.1 | hypothetical protein | - |
| ACNM7T_RS08985 (ACNM7T_08985) | - | 1828331..1829038 (+) | 708 | WP_007410381.1 | poly-gamma-glutamate hydrolase family protein | - |
| ACNM7T_RS08990 (ACNM7T_08990) | - | 1829098..1829550 (+) | 453 | WP_007410380.1 | OsmC family protein | - |
| ACNM7T_RS08995 (ACNM7T_08995) | - | 1829564..1829917 (-) | 354 | WP_007410379.1 | DMT family transporter | - |
| ACNM7T_RS09000 (ACNM7T_09000) | - | 1829935..1830249 (-) | 315 | WP_007410378.1 | DMT family transporter | - |
| ACNM7T_RS09005 (ACNM7T_09005) | - | 1830388..1830693 (-) | 306 | WP_015239898.1 | hypothetical protein | - |
| ACNM7T_RS09010 (ACNM7T_09010) | - | 1830795..1831199 (+) | 405 | WP_032866019.1 | YmaF family protein | - |
| ACNM7T_RS09015 (ACNM7T_09015) | miaA | 1831298..1832242 (+) | 945 | WP_007410375.1 | tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA | - |
| ACNM7T_RS09020 (ACNM7T_09020) | hfq | 1832282..1832503 (+) | 222 | WP_003154064.1 | RNA chaperone Hfq | - |
| ACNM7T_RS09025 (ACNM7T_09025) | - | 1832600..1832875 (+) | 276 | WP_007410374.1 | YmzC family protein | - |
| ACNM7T_RS09030 (ACNM7T_09030) | - | 1832958..1833173 (+) | 216 | WP_003154062.1 | hypothetical protein | - |
| ACNM7T_RS09035 (ACNM7T_09035) | nrdI | 1833433..1833825 (+) | 393 | WP_003154061.1 | class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI | - |
| ACNM7T_RS09040 (ACNM7T_09040) | nrdE | 1833785..1835887 (+) | 2103 | WP_007611605.1 | class 1b ribonucleoside-diphosphate reductase subunit alpha | - |
| ACNM7T_RS09045 (ACNM7T_09045) | nrdF | 1835905..1836894 (+) | 990 | WP_012117608.1 | class 1b ribonucleoside-diphosphate reductase subunit beta | - |
| ACNM7T_RS09050 (ACNM7T_09050) | - | 1836943..1837563 (+) | 621 | WP_012117609.1 | hypothetical protein | - |
| ACNM7T_RS09055 (ACNM7T_09055) | - | 1837613..1838371 (-) | 759 | WP_012117610.1 | N-acetylmuramoyl-L-alanine amidase | - |
Sequence
Protein
Download Length: 144 a.a. Molecular weight: 15514.44 Da Isoelectric Point: 7.2418
>NTDB_id=1103786 ACNM7T_RS08955 WP_015417516.1 1823209..1823643(+) (nucA/comI) [Bacillus amyloliquefaciens strain Scm]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ
Nucleotide
Download Length: 435 bp
>NTDB_id=1103786 ACNM7T_RS08955 WP_015417516.1 1823209..1823643(+) (nucA/comI) [Bacillus amyloliquefaciens strain Scm]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
57.258 |
86.111 |
0.493 |