Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACAK34_RS01775 Genome accession   NZ_AP031582
Coordinates   368622..369362 (+) Length   246 a.a.
NCBI ID   WP_000076097.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain JUNP499     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 363622..374362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAK34_RS01760 (JUNP499_0360) ponA 364210..366765 (-) 2556 WP_000736686.1 penicillin-binding protein PBP1a -
  ACAK34_RS01765 (JUNP499_0361) pilM 366926..367984 (+) 1059 WP_005119265.1 pilus assembly protein PilM Machinery gene
  ACAK34_RS01770 (JUNP499_0362) pilN 367984..368625 (+) 642 WP_000201231.1 PilN domain-containing protein Machinery gene
  ACAK34_RS01775 (JUNP499_0363) pilO 368622..369362 (+) 741 WP_000076097.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACAK34_RS01780 (JUNP499_0364) pilP 369373..369900 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  ACAK34_RS01785 (JUNP499_0365) pilQ 369963..372128 (+) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  ACAK34_RS01790 (JUNP499_0366) aroK 372140..372709 (+) 570 WP_000643712.1 shikimate kinase AroK -
  ACAK34_RS01795 (JUNP499_0367) aroB 372738..373820 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27785.55 Da        Isoelectric Point: 4.8357

>NTDB_id=110346 ACAK34_RS01775 WP_000076097.1 368622..369362(+) (pilO) [Acinetobacter baumannii strain JUNP499]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGFFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTIHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=110346 ACAK34_RS01775 WP_000076097.1 368622..369362(+) (pilO) [Acinetobacter baumannii strain JUNP499]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTTTTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATTCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.187

100

0.992
  comO Acinetobacter baylyi ADP1

69.008

98.374

0.679

Multiple sequence alignment