Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACNH6H_RS09685 Genome accession   NZ_CP182947
Coordinates   2167502..2169046 (+) Length   514 a.a.
NCBI ID   WP_418317293.1    Uniprot ID   -
Organism   Piscinibacter sakaiensis strain 201-F6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2162502..2174046
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6H_RS09660 (ACNH6H_09660) argS 2162757..2164502 (+) 1746 WP_418317289.1 arginine--tRNA ligase -
  ACNH6H_RS09665 (ACNH6H_09665) - 2164499..2165218 (+) 720 WP_418317290.1 SPOR domain-containing protein -
  ACNH6H_RS09670 (ACNH6H_09670) - 2165336..2165977 (+) 642 WP_418317291.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACNH6H_RS09675 (ACNH6H_09675) lptA 2166080..2166703 (+) 624 WP_418317292.1 lipopolysaccharide transport periplasmic protein LptA -
  ACNH6H_RS09680 (ACNH6H_09680) lptB 2166793..2167491 (+) 699 WP_418321275.1 LPS export ABC transporter ATP-binding protein -
  ACNH6H_RS09685 (ACNH6H_09685) rpoN/rpoN1 2167502..2169046 (+) 1545 WP_418317293.1 RNA polymerase factor sigma-54 Machinery gene
  ACNH6H_RS09690 (ACNH6H_09690) - 2169173..2170438 (+) 1266 WP_418317294.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACNH6H_RS09695 (ACNH6H_09695) - 2170463..2170885 (-) 423 WP_418317295.1 YidB family protein -
  ACNH6H_RS09700 (ACNH6H_09700) - 2170965..2171729 (-) 765 WP_418317296.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACNH6H_RS09705 (ACNH6H_09705) - 2171799..2173475 (-) 1677 WP_418317297.1 long-chain-fatty-acid--CoA ligase -

Sequence

Protein


Download         Length: 514 a.a.        Molecular weight: 56714.14 Da        Isoelectric Point: 4.7794

>NTDB_id=1103405 ACNH6H_RS09685 WP_418317293.1 2167502..2169046(+) (rpoN/rpoN1) [Piscinibacter sakaiensis strain 201-F6]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEMEEESVSLAEPLAERLSAAERAGERESERAGE
SGADAADDAPAIDGAEFGATERDDWENGTERDDFDGIRETPSLNSPGSGSGDDSDFEAQDRDQFDISLAEHLRRQLLGMR
LSAEDAAAIHVLIESLSEDGYLADSLEEIAAGLVDDPEQREELVDRLRCALIWLQGMEPAGVGARDLGECLCLQLRQLPR
SQAQAIAIIVCRNHLELLARRDIKRLMSATGADEELLREAQALIVALEPKPGRAFSRAEANIIIPDVIVQKSGRGWKVVL
NPDVMPKLRINDLYAQAIRQQRGGRGGGGLNGSHAGLSSRLQEARWFMKNILQRFDTIQRVSQAIVERQKAFFSHGEIAM
KPLVLREIADELGLHESTISRVTTAKYMTTPFGTFELKYFFGSSLNTDVGGNASSTAVRALIKQFVAAENATKPLSDSQL
STMLEEQGIKVARRTVAKYREALKIAPANLRKAM

Nucleotide


Download         Length: 1545 bp        

>NTDB_id=1103405 ACNH6H_RS09685 WP_418317293.1 2167502..2169046(+) (rpoN/rpoN1) [Piscinibacter sakaiensis strain 201-F6]
ATGAAACCCTCGTTGCAGGTCCGGCTGTCGCAGCACCTGGCGCTGACGCCGCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAGTTGCACCAGGAAGTCGAGCAGATGCTCGAGCAGAATCCCTTCCTCGAGATGGAAGAGGAGTCGG
TGTCGCTCGCCGAGCCACTCGCCGAACGATTGAGCGCGGCTGAACGTGCCGGCGAACGTGAATCGGAGCGGGCTGGCGAA
TCCGGCGCCGATGCGGCCGACGACGCGCCGGCGATCGATGGTGCCGAATTCGGCGCGACCGAGCGCGACGACTGGGAAAA
CGGCACCGAACGCGACGATTTCGACGGCATCCGCGAAACGCCGTCGCTGAACTCGCCCGGCAGCGGAAGCGGCGACGACA
GCGACTTCGAGGCGCAGGACCGTGACCAGTTCGACATCAGCCTGGCCGAACATCTGCGCCGGCAGCTGCTCGGCATGCGC
CTGTCGGCCGAAGATGCGGCGGCCATCCATGTGCTGATCGAATCGCTGAGCGAAGACGGTTATCTGGCCGATTCGCTGGA
GGAGATTGCCGCCGGGCTGGTCGACGATCCCGAGCAGCGCGAGGAACTGGTCGATCGCCTGCGATGTGCGCTGATCTGGC
TGCAGGGCATGGAACCGGCCGGTGTCGGCGCCCGCGACCTAGGCGAATGCCTGTGCCTGCAGTTGCGCCAGCTGCCGCGC
AGCCAGGCGCAGGCAATCGCGATCATCGTCTGCCGCAACCACCTCGAACTGCTCGCTCGCCGCGACATCAAGCGGCTGAT
GTCGGCCACCGGTGCCGACGAGGAGCTGCTGCGCGAGGCACAGGCGCTGATCGTCGCGCTCGAGCCGAAACCGGGCCGGG
CCTTCAGCCGCGCCGAAGCGAACATCATCATCCCCGATGTGATCGTGCAGAAATCGGGCCGCGGCTGGAAGGTGGTGCTC
AATCCCGACGTGATGCCGAAATTGCGCATCAATGACCTTTATGCGCAGGCGATCCGCCAGCAACGCGGCGGACGCGGCGG
CGGCGGCCTGAACGGCAGCCACGCAGGCCTCAGCTCGCGGCTGCAGGAAGCGCGCTGGTTCATGAAAAATATCCTGCAGC
GCTTCGACACCATCCAGCGGGTTTCGCAGGCGATCGTCGAGCGCCAGAAAGCCTTCTTCAGCCACGGCGAGATCGCGATG
AAGCCGCTGGTGCTGCGCGAAATCGCCGACGAACTCGGCCTGCACGAATCGACGATCTCGCGGGTGACGACGGCCAAATA
CATGACGACGCCGTTCGGCACCTTCGAGCTGAAATATTTTTTCGGCTCGTCGCTGAATACCGACGTTGGTGGCAATGCGT
CGAGCACCGCGGTGCGCGCGTTGATCAAGCAGTTCGTCGCCGCCGAGAATGCCACCAAGCCGCTGTCGGACAGTCAGCTG
TCGACGATGCTCGAGGAGCAGGGCATCAAGGTCGCGCGGCGCACCGTCGCAAAATACCGCGAGGCGCTGAAGATTGCGCC
GGCCAATCTGCGCAAGGCAATGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(145-334)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(357-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.817

100

0.549

Multiple sequence alignment