Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACNPWN_RS00955 Genome accession   NZ_CP182825
Coordinates   199751..201118 (+) Length   455 a.a.
NCBI ID   WP_119864261.1    Uniprot ID   -
Organism   Clostridium isatidis strain BK32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 194751..206118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNPWN_RS00930 (ACNPWN_00930) - 195690..197405 (+) 1716 WP_418223087.1 NADH-dependent [FeFe] hydrogenase, group A6 -
  ACNPWN_RS00935 (ACNPWN_00935) - 197441..197668 (-) 228 WP_119864258.1 glutaredoxin family protein -
  ACNPWN_RS00940 (ACNPWN_00940) - 197777..197938 (-) 162 WP_164704059.1 hypothetical protein -
  ACNPWN_RS00945 (ACNPWN_00945) - 198075..198797 (+) 723 WP_119864259.1 GntR family transcriptional regulator -
  ACNPWN_RS00950 (ACNPWN_00950) nagB 198794..199525 (+) 732 WP_119864260.1 glucosamine-6-phosphate deaminase -
  ACNPWN_RS00955 (ACNPWN_00955) radA 199751..201118 (+) 1368 WP_119864261.1 DNA repair protein RadA Machinery gene
  ACNPWN_RS00960 (ACNPWN_00960) disA 201137..202201 (+) 1065 WP_418223088.1 DNA integrity scanning diadenylate cyclase DisA -
  ACNPWN_RS00965 (ACNPWN_00965) - 202271..202660 (-) 390 WP_119864263.1 DUF1573 domain-containing protein -
  ACNPWN_RS00970 (ACNPWN_00970) - 202907..204001 (+) 1095 WP_119864264.1 PIN/TRAM domain-containing protein -
  ACNPWN_RS00975 (ACNPWN_00975) ispD 204017..204685 (+) 669 WP_119864265.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50603.35 Da        Isoelectric Point: 6.5190

>NTDB_id=1102649 ACNPWN_RS00955 WP_119864261.1 199751..201118(+) (radA) [Clostridium isatidis strain BK32]
MPKIKSVYVCQECGYESAKWLGKCPDCNNWNTLVEEVRDNKKELKSAINTVRILENSPKSIGDIQSGEKKRYNTGLEELN
RVLGGGLVRGSLTLISGDPGIGKSTLLLQTSNYIASNYGKVLYVSGEESEEQIKIRGDRLGVNSNNLYILSETNLDLIEA
HIEELKPVFVIIDSIQTVYKESITSAPGSVSQVRECSNAIMRIGKNNNIPLFIVAHVTKQGELAGPRVLEHIVDTVLYFE
GERTEEFRVLRTMKNRFGTTSEIGVFEMSEEGLVEIYDPSKIFLEETNFNQEGSSVIGIMEGTRPILVEMQALATETKMN
IPIRTGIGIDFQRLRLIIAVLEKKLRVPFYKYDVYANVVGGLRLEGTTGDLGLALSLISSIKNKPFKLEKVLIIGEVGLT
GEIRPVASCDRLIREAEKMGFKHAIIPERNKDKVKSKTISIIGVNNLTEAINNIF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=1102649 ACNPWN_RS00955 WP_119864261.1 199751..201118(+) (radA) [Clostridium isatidis strain BK32]
ATGCCTAAAATAAAATCGGTATATGTTTGTCAGGAATGCGGATACGAGTCGGCAAAATGGCTTGGGAAATGCCCGGATTG
TAATAATTGGAATACTTTGGTGGAGGAAGTTAGAGATAATAAAAAAGAACTAAAAAGTGCAATAAATACAGTAAGAATAT
TAGAAAATTCTCCTAAAAGCATAGGAGATATACAATCAGGTGAAAAGAAAAGATATAATACTGGATTAGAAGAATTAAAT
AGAGTGTTGGGAGGAGGACTTGTGAGAGGATCTTTAACCTTGATTTCAGGAGATCCCGGAATAGGTAAGTCAACACTTTT
ACTTCAAACATCAAATTATATTGCCAGCAATTATGGTAAAGTTTTATATGTATCAGGGGAGGAATCTGAGGAACAAATAA
AAATTCGAGGCGATAGGCTTGGTGTTAATTCAAATAATTTATACATACTATCAGAAACAAACCTAGATTTAATAGAAGCA
CATATAGAAGAATTAAAACCTGTCTTTGTAATAATAGATTCGATACAAACAGTATATAAGGAAAGCATAACTTCAGCACC
AGGAAGTGTATCTCAAGTAAGAGAATGTTCAAATGCAATAATGAGAATAGGAAAAAACAATAATATTCCTTTATTTATAG
TTGCTCATGTAACAAAGCAGGGAGAACTTGCAGGGCCAAGGGTATTAGAGCATATAGTAGATACTGTATTGTATTTTGAA
GGAGAACGTACTGAAGAATTTAGGGTTTTAAGAACAATGAAAAATAGGTTTGGTACAACTAGTGAAATTGGCGTCTTTGA
AATGTCTGAGGAAGGTTTAGTGGAAATATACGATCCGTCAAAGATATTTTTAGAGGAAACAAATTTCAATCAGGAAGGTT
CATCAGTTATAGGAATAATGGAAGGTACAAGACCTATTTTAGTAGAAATGCAGGCTTTAGCTACTGAAACTAAGATGAAC
ATTCCAATAAGAACTGGTATAGGAATAGATTTTCAAAGGCTTAGACTAATAATAGCCGTTTTAGAAAAAAAGCTAAGAGT
ACCTTTTTATAAATACGATGTATATGCAAATGTTGTTGGTGGCCTTAGATTAGAAGGAACAACAGGTGATTTAGGGTTAG
CATTATCTTTGATATCAAGTATTAAAAATAAACCTTTTAAATTAGAGAAAGTTTTAATAATAGGAGAAGTTGGACTTACT
GGAGAAATTAGACCAGTTGCATCTTGTGATAGGCTTATAAGAGAAGCAGAAAAAATGGGTTTTAAACATGCTATTATTCC
AGAGAGAAATAAAGATAAGGTAAAAAGTAAAACAATAAGCATAATTGGAGTAAATAATTTAACTGAGGCAATTAATAATA
TATTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(351-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

52.298

100

0.525
  radA Streptococcus pneumoniae Rx1

48.904

100

0.49
  radA Streptococcus pneumoniae D39

48.904

100

0.49
  radA Streptococcus pneumoniae R6

48.904

100

0.49
  radA Streptococcus pneumoniae TIGR4

48.904

100

0.49
  radA Streptococcus mitis SK321

48.684

100

0.488
  radA Streptococcus mitis NCTC 12261

48.684

100

0.488

Multiple sequence alignment