Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACM641_RS00640 Genome accession   NZ_CP182815
Coordinates   134191..135567 (+) Length   458 a.a.
NCBI ID   WP_008180607.1    Uniprot ID   -
Organism   Lysinibacillus fusiformis strain A15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 129191..140567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM641_RS00620 - 129533..130003 (+) 471 WP_031416734.1 CtsR family transcriptional regulator -
  ACM641_RS00625 - 130017..130559 (+) 543 WP_025116467.1 UvrB/UvrC motif-containing protein -
  ACM641_RS00630 - 130556..131665 (+) 1110 WP_144788658.1 protein arginine kinase -
  ACM641_RS00635 - 131655..134099 (+) 2445 WP_418301861.1 ATP-dependent Clp protease ATP-binding subunit -
  ACM641_RS00640 radA 134191..135567 (+) 1377 WP_008180607.1 DNA repair protein RadA Machinery gene
  ACM641_RS00645 - 135783..136868 (+) 1086 WP_418301862.1 PIN/TRAM domain-containing protein -
  ACM641_RS00650 ispD 136880..137575 (+) 696 WP_418301863.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACM641_RS00655 ispF 137963..138439 (+) 477 WP_008180601.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ACM641_RS00660 gltX 138528..139988 (+) 1461 WP_418301864.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50197.03 Da        Isoelectric Point: 7.3825

>NTDB_id=1102531 ACM641_RS00640 WP_008180607.1 134191..135567(+) (radA) [Lysinibacillus fusiformis strain A15]
MAKKKTKFVCSGCGYESAKWMGRCPGCGEWNKMVEEVEVIAKGPRGAFQHSATVTQKAIPIIQVEASEESRVATEMGELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLSNKGHRVLYISGEESIRQTKLRAERLGVISQELYIYSETNLEFLNQ
TIEEVQPKFVIVDSIQTVFHPEVTSAPGSVSQVRECTAELMRIAKTKGIAIFLVGHVTKEGQIAGPRILEHMVDTVLYFE
GERHHNHRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTIVASMEGTRPILVEIQSLVTPTSFN
YPKRMATGVDQNRVQLLMAVLEKRMGLMLQAQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKDQAVRATDCFVGEVGLTG
EVRRVSRIEQRVIEAAKLGFKRVFIPTSNIGGWDFPQGIEIIGVETIKDALNACFREL

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1102531 ACM641_RS00640 WP_008180607.1 134191..135567(+) (radA) [Lysinibacillus fusiformis strain A15]
TTGGCAAAGAAAAAAACGAAATTTGTATGTTCAGGCTGTGGCTATGAGTCAGCAAAATGGATGGGAAGATGTCCAGGGTG
TGGGGAATGGAATAAGATGGTGGAGGAAGTAGAAGTGATTGCGAAAGGACCACGTGGGGCTTTCCAGCATTCCGCTACTG
TTACTCAAAAGGCAATCCCTATTATACAGGTGGAAGCTAGTGAGGAGTCCCGTGTAGCGACAGAAATGGGCGAGCTGAAC
CGTGTATTAGGCGGTGGTATTGTGCCTGGCTCTCTAGTATTAATAGGTGGTGATCCCGGGATTGGAAAATCCACATTATT
ACTACAAGTTTCAGCTTTGCTCTCTAATAAAGGTCATCGTGTGCTGTATATTTCTGGTGAGGAATCTATCCGTCAAACGA
AGCTACGTGCGGAGCGTTTAGGGGTAATCTCTCAGGAGTTGTACATTTATTCCGAAACAAATCTTGAGTTTTTGAATCAA
ACGATTGAGGAAGTACAGCCGAAATTTGTCATTGTCGATTCCATACAGACTGTCTTTCATCCAGAGGTAACAAGTGCACC
AGGTAGTGTCTCACAGGTCCGTGAGTGTACAGCTGAGCTTATGCGAATTGCTAAGACAAAAGGAATTGCGATCTTCTTAG
TTGGGCATGTTACGAAAGAGGGGCAAATAGCAGGGCCTCGTATTTTAGAACATATGGTGGATACAGTCCTGTATTTTGAA
GGGGAAAGACATCATAATCATCGTATTTTACGAAGTCAAAAAAACCGCTTCGGCTCTACAAATGAAATTGCTATATTTGA
AATGCTACAGGGTGGGCTAAAAGAGGTTTTGAACCCATCTGAGCTATTTTTACAGGAGCGTTCACAAGGAGCTGCGGGCT
CCACGATTGTTGCCTCAATGGAGGGAACAAGGCCAATTCTCGTTGAAATACAGTCGCTAGTGACGCCTACCAGCTTTAAT
TACCCCAAACGGATGGCAACCGGGGTTGATCAAAATAGAGTGCAATTGCTGATGGCCGTACTTGAAAAGCGTATGGGTCT
CATGCTACAAGCACAGGATGCATATATTAAAGTTGCTGGTGGTGTAAAATTAGACGAACCCGCCATCGATTTAGCTGTTT
TGACAAGCATCGTTTCTAGCTTTAAGGATCAGGCTGTGCGAGCAACGGATTGCTTTGTTGGTGAGGTGGGTCTGACAGGA
GAGGTGCGTCGAGTATCTCGTATTGAGCAACGTGTCATTGAAGCTGCGAAGCTTGGTTTTAAAAGAGTATTCATTCCTAC
GTCCAATATAGGTGGTTGGGATTTTCCACAAGGAATTGAAATTATCGGGGTAGAAACGATCAAGGATGCATTAAATGCCT
GCTTTAGAGAGTTATAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(350-432)

AAA domain (AAA_25)

(66-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

70.925

99.127

0.703
  radA Streptococcus pneumoniae Rx1

63.158

99.563

0.629
  radA Streptococcus pneumoniae D39

63.158

99.563

0.629
  radA Streptococcus pneumoniae R6

63.158

99.563

0.629
  radA Streptococcus pneumoniae TIGR4

63.158

99.563

0.629
  radA Streptococcus mitis NCTC 12261

63.158

99.563

0.629
  radA Streptococcus mitis SK321

63.158

99.563

0.629

Multiple sequence alignment