Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   ACNFKI_RS13340 Genome accession   NZ_CP182571
Coordinates   2680588..2681388 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain DY201     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2675588..2686388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFKI_RS13310 (ACNFKI_13310) - 2676138..2676770 (+) 633 WP_014418348.1 helix-turn-helix domain-containing protein -
  ACNFKI_RS13315 (ACNFKI_13315) - 2677061..2677198 (+) 138 WP_014470646.1 hypothetical protein -
  ACNFKI_RS13320 (ACNFKI_13320) - 2677292..2678410 (+) 1119 WP_015388019.1 NADH-dependent flavin oxidoreductase -
  ACNFKI_RS13325 (ACNFKI_13325) - 2678536..2678748 (+) 213 WP_003153183.1 spore germination protein -
  ACNFKI_RS13330 (ACNFKI_13330) - 2678874..2679086 (+) 213 WP_014305392.1 spore germination protein -
  ACNFKI_RS13335 (ACNFKI_13335) alr 2679261..2680442 (+) 1182 WP_015388018.1 alanine racemase -
  ACNFKI_RS13340 (ACNFKI_13340) spo0A 2680588..2681388 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  ACNFKI_RS13345 (ACNFKI_13345) spoIVB 2681669..2682949 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  ACNFKI_RS13350 (ACNFKI_13350) recN 2683112..2684842 (-) 1731 WP_024085591.1 DNA repair protein RecN Machinery gene
  ACNFKI_RS13355 (ACNFKI_13355) ahrC 2684874..2685323 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  ACNFKI_RS13360 (ACNFKI_13360) - 2685420..2686265 (-) 846 WP_015388017.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=1102276 ACNFKI_RS13340 WP_003153177.1 2680588..2681388(-) (spo0A) [Bacillus velezensis strain DY201]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1102276 ACNFKI_RS13340 WP_003153177.1 2680588..2681388(-) (spo0A) [Bacillus velezensis strain DY201]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCCGATGTGCTCG
TGTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGTGAATCAGAGCTTGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCATCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCGGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAACCAACCAACTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment