Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ACNFKI_RS06065 Genome accession   NZ_CP182571
Coordinates   1156668..1158497 (+) Length   609 a.a.
NCBI ID   WP_003155028.1    Uniprot ID   -
Organism   Bacillus velezensis strain DY201     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1151668..1163497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFKI_RS06040 (ACNFKI_06040) - 1152331..1152909 (+) 579 WP_003155035.1 GNAT family N-acetyltransferase -
  ACNFKI_RS06045 (ACNFKI_06045) spx 1153088..1153483 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  ACNFKI_RS06050 (ACNFKI_06050) - 1153541..1154197 (-) 657 WP_003155033.1 TerC family protein -
  ACNFKI_RS06055 (ACNFKI_06055) mecA 1154473..1155129 (+) 657 WP_003155032.1 adaptor protein MecA Regulator
  ACNFKI_RS06060 (ACNFKI_06060) - 1155280..1156440 (+) 1161 WP_060386867.1 competence protein CoiA family protein -
  ACNFKI_RS06065 (ACNFKI_06065) pepF 1156668..1158497 (+) 1830 WP_003155028.1 oligoendopeptidase F Regulator
  ACNFKI_RS06070 (ACNFKI_06070) - 1158535..1158702 (-) 168 WP_003155026.1 hypothetical protein -
  ACNFKI_RS06075 (ACNFKI_06075) spxH 1158988..1159890 (-) 903 WP_003155024.1 protease adaptor protein SpxH -
  ACNFKI_RS06080 (ACNFKI_06080) - 1159887..1160285 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  ACNFKI_RS06085 (ACNFKI_06085) - 1160514..1161200 (-) 687 WP_044053101.1 lytic transglycosylase domain-containing protein -
  ACNFKI_RS06090 (ACNFKI_06090) - 1161205..1161780 (-) 576 WP_003155021.1 CYTH domain-containing protein -
  ACNFKI_RS06095 (ACNFKI_06095) - 1161905..1162270 (+) 366 WP_025851933.1 hypothetical protein -
  ACNFKI_RS06100 (ACNFKI_06100) yjbM 1162298..1162933 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69799.43 Da        Isoelectric Point: 5.2024

>NTDB_id=1102264 ACNFKI_RS06065 WP_003155028.1 1156668..1158497(+) (pepF) [Bacillus velezensis strain DY201]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=1102264 ACNFKI_RS06065 WP_003155028.1 1156668..1158497(+) (pepF) [Bacillus velezensis strain DY201]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGATTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATTCCGAATTTATCTTCATTTAAAGGCA
CGCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCTCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTTATTC
TGGAAAAAGAGGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCTGAAGCGCTGTCTTCGCCTTCCAATACATTCAGTGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTAAAAAAAGATAATTTTTACGCCCGTGTGAAAAAGTATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATTCCTGAAGAGGTTTACGATAACCTTATCAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTAACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTAACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAACTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488

Multiple sequence alignment