Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACNFKI_RS01755 Genome accession   NZ_CP182571
Coordinates   321600..322037 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain DY201     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 316600..327037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFKI_RS01715 (ACNFKI_01715) - 317179..317343 (-) 165 WP_374107008.1 hypothetical protein -
  ACNFKI_RS01720 (ACNFKI_01720) - 317457..318659 (+) 1203 WP_025853783.1 GTP-binding protein -
  ACNFKI_RS01725 (ACNFKI_01725) - 318724..319860 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  ACNFKI_RS01730 (ACNFKI_01730) - 319875..320309 (+) 435 WP_003156592.1 RDD family protein -
  ACNFKI_RS01735 (ACNFKI_01735) - 320426..320701 (+) 276 WP_371745793.1 YckD family protein -
  ACNFKI_RS01740 (ACNFKI_01740) - 320805..320921 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  ACNFKI_RS01745 (ACNFKI_01745) - 320920..321139 (+) 220 Protein_303 family 1 glycosylhydrolase -
  ACNFKI_RS01750 (ACNFKI_01750) nin/comJ 321180..321578 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  ACNFKI_RS01755 (ACNFKI_01755) nucA/comI 321600..322037 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  ACNFKI_RS01760 (ACNFKI_01760) hxlB 322396..322953 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  ACNFKI_RS01765 (ACNFKI_01765) hxlA 322950..323585 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  ACNFKI_RS01770 (ACNFKI_01770) - 323817..324179 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1102246 ACNFKI_RS01755 WP_003156588.1 321600..322037(-) (nucA/comI) [Bacillus velezensis strain DY201]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1102246 ACNFKI_RS01755 WP_003156588.1 321600..322037(-) (nucA/comI) [Bacillus velezensis strain DY201]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment