Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACNI65_RS24400 Genome accession   NZ_CP182288
Coordinates   5352407..5353930 (+) Length   507 a.a.
NCBI ID   WP_416760027.1    Uniprot ID   -
Organism   Roseateles sp. So40a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5347407..5358930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNI65_RS24380 (ACNI65_24380) - 5348919..5349998 (+) 1080 WP_416760022.1 type IV toxin-antitoxin system AbiEi family antitoxin -
  ACNI65_RS24385 (ACNI65_24385) - 5349986..5350858 (+) 873 WP_416760023.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
  ACNI65_RS24390 (ACNI65_24390) lptA 5351005..5351628 (+) 624 WP_416760024.1 lipopolysaccharide transport periplasmic protein LptA -
  ACNI65_RS24395 (ACNI65_24395) lptB 5351625..5352392 (+) 768 WP_416760025.1 LPS export ABC transporter ATP-binding protein -
  ACNI65_RS24400 (ACNI65_24400) rpoN/rpoN1 5352407..5353930 (+) 1524 WP_416760027.1 RNA polymerase factor sigma-54 Machinery gene
  ACNI65_RS24405 (ACNI65_24405) - 5354055..5355341 (+) 1287 WP_416760028.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACNI65_RS24410 (ACNI65_24410) - 5355419..5355664 (+) 246 WP_416760029.1 type II toxin-antitoxin system ParD family antitoxin -
  ACNI65_RS24415 (ACNI65_24415) - 5355689..5355979 (+) 291 WP_416760030.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACNI65_RS24420 (ACNI65_24420) - 5355982..5356728 (-) 747 WP_416760032.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACNI65_RS24425 (ACNI65_24425) - 5356732..5357289 (-) 558 WP_416760033.1 NADPH-dependent FMN reductase -
  ACNI65_RS24430 (ACNI65_24430) - 5357344..5357907 (-) 564 WP_416760035.1 hypothetical protein -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 56330.37 Da        Isoelectric Point: 4.5391

>NTDB_id=1100997 ACNI65_RS24400 WP_416760027.1 5352407..5353930(+) (rpoN/rpoN1) [Roseateles sp. So40a]
MRPTLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLASNPLLETDEEFDAPLPTATQERLTEREEREERVAQTES
DSSSGDAPAGEEMRAEEFGTTERDDWENGTEGDDFDGIRETPGSAPASSGDDDDRPETEAPSESLQDVLKAQIPGMTLSA
VDRAALLALIESLNDDGYLEDPIEELVVAILGEDADLEQREDLLDRFKVALRWLQSMEPAGVGARDLGECLELQLRVLPR
SPEQALAIVVCRRHLDLLAKRDWRRLMSLTGADEELLRDAQSLIAGLEPKPGRRYAKSDNRPVIPDVIVQKSGRNFRVSI
NPEVLPKLRINDLYAQALRATRGGVSNSPLGGQLQEARWFIKNIQQRFDTIQRVSEAIVERQRGFFTHGELAMKPLVLRE
IADELGLHESTISRVTTAKYMATPWGTFELKYFFGSSLSTEAGGNASSTAVRALIKQFIAAEDTASPLSDSQLSTMLGEQ
GITVARRTVAKYREALKLAPANLRKAR

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=1100997 ACNI65_RS24400 WP_416760027.1 5352407..5353930(+) (rpoN/rpoN1) [Roseateles sp. So40a]
ATGAGGCCGACGCTACAGGTTCGCCTGTCCCAGCATCTTGCGCTGACGCCTCAGCTGCAGCAGTCCATCCGTCTGTTGCA
GCTGTCGACGCTGGAGCTCCACCAGGAGGTGGAGCAGATGCTCGCGAGCAACCCGCTGCTGGAGACCGACGAGGAATTCG
ACGCCCCGCTGCCGACCGCGACGCAGGAGCGCCTGACCGAACGCGAGGAGCGCGAAGAACGCGTCGCGCAGACCGAGTCC
GACAGCAGCAGCGGCGACGCGCCGGCGGGCGAGGAGATGCGGGCCGAGGAATTCGGCACCACCGAGCGCGACGACTGGGA
GAACGGCACCGAGGGCGACGACTTCGACGGCATCCGCGAGACGCCGGGCAGCGCGCCCGCCTCGTCGGGCGACGACGACG
ACCGGCCCGAGACTGAAGCGCCGTCGGAGTCGCTGCAGGACGTGCTCAAGGCACAGATCCCCGGCATGACGCTCAGCGCG
GTCGACCGCGCGGCGCTGCTCGCGCTGATCGAATCGCTCAATGACGACGGCTACCTCGAGGATCCGATCGAGGAACTGGT
CGTGGCGATCCTCGGCGAGGACGCGGACCTCGAGCAGCGCGAGGACCTGCTGGACCGGTTCAAGGTCGCGCTCAGATGGC
TGCAGTCGATGGAGCCGGCCGGCGTCGGCGCGCGCGACCTGGGCGAATGCCTGGAGCTGCAACTGCGCGTCCTGCCCAGA
TCGCCGGAACAGGCGCTGGCCATCGTTGTCTGCCGGCGCCATCTGGACCTGCTGGCCAAGCGGGACTGGCGCCGCCTGAT
GTCGCTGACCGGCGCGGACGAGGAGCTGCTGCGCGACGCGCAGTCGCTGATCGCGGGCCTGGAGCCCAAGCCGGGCCGCC
GTTACGCGAAGAGCGACAACCGGCCGGTGATTCCGGACGTGATCGTGCAGAAGTCGGGCCGCAACTTCCGCGTGTCGATC
AATCCGGAGGTGCTGCCCAAACTGCGCATCAACGACCTCTACGCACAGGCGCTGCGCGCCACGCGGGGCGGCGTCAGCAA
CTCGCCGCTGGGCGGGCAACTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCAGCGCG
TCAGCGAGGCGATCGTCGAGCGCCAGCGCGGGTTCTTCACCCACGGCGAGCTGGCGATGAAGCCGCTGGTGCTGCGCGAG
ATCGCCGATGAGCTGGGCCTGCACGAGTCCACCATCTCCCGCGTGACGACGGCCAAGTACATGGCCACGCCGTGGGGCAC
CTTCGAGCTCAAGTACTTCTTCGGCTCCAGTCTGTCGACCGAGGCCGGCGGCAACGCATCGAGCACGGCGGTGCGGGCGC
TGATCAAGCAGTTCATCGCTGCCGAGGACACCGCGAGCCCGCTGTCGGACAGCCAGCTGTCGACGATGCTCGGCGAGCAG
GGCATCACGGTGGCGCGGCGCACGGTCGCGAAGTACCGCGAGGCGCTCAAGCTCGCGCCCGCGAACCTGCGCAAGGCCCG
ATAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-334)

Sigma-54, DNA binding domain (Sigma54_DBD)

(353-505)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.568

100

0.527

Multiple sequence alignment