Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABXS94_RS02785 Genome accession   NZ_AP031566
Coordinates   604041..604850 (+) Length   269 a.a.
NCBI ID   WP_353675501.1    Uniprot ID   -
Organism   Acinetobacter towneri strain PT23-B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 599041..609850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXS94_RS02755 (PT23B2_05310) - 599196..599621 (+) 426 WP_131260763.1 hypothetical protein -
  ABXS94_RS02760 (PT23B2_05320) - 599862..601145 (+) 1284 WP_353675499.1 glycosyltransferase family 1 protein -
  ABXS94_RS02765 (PT23B2_05330) - 601171..601722 (+) 552 WP_279186092.1 phosphatase PAP2 family protein -
  ABXS94_RS02770 (PT23B2_05340) iscX 601824..602024 (+) 201 WP_004975089.1 Fe-S cluster assembly protein IscX -
  ABXS94_RS02775 (PT23B2_05350) ndk 602186..602617 (+) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  ABXS94_RS02780 (PT23B2_05360) rlmN 602754..603986 (+) 1233 WP_353675500.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABXS94_RS02785 (PT23B2_05370) pilF 604041..604850 (+) 810 WP_353675501.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABXS94_RS02790 (PT23B2_05380) - 604841..605650 (+) 810 WP_353675502.1 RodZ domain-containing protein -
  ABXS94_RS02795 (PT23B2_05390) ispG 605673..606794 (+) 1122 WP_131260758.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABXS94_RS02800 (PT23B2_05400) hisS 606798..608093 (+) 1296 WP_353675503.1 histidine--tRNA ligase -
  ABXS94_RS02805 (PT23B2_05410) - 608124..608825 (+) 702 WP_131260757.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 30226.25 Da        Isoelectric Point: 9.2875

>NTDB_id=109997 ABXS94_RS02785 WP_353675501.1 604041..604850(+) (pilF) [Acinetobacter towneri strain PT23-B2]
MNKPVFKFAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSSANMMM
GVLLQQEGSKLNLEKADAYFKRAIAADPQNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKL
GDVANAEKSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVL
VNQLRALFPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 810 bp        

>NTDB_id=109997 ABXS94_RS02785 WP_353675501.1 604041..604850(+) (pilF) [Acinetobacter towneri strain PT23-B2]
TTGAATAAGCCTGTATTTAAATTCGCACAAATTACAACCGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCAC
AGCGCCAACGCTCGGACAAAAAGACCCAGAAAAAGCAGTACAAGTACGCACTCAACTTGCCGCAGAATATTTAAAAAATG
GCGACTTAGATTCTGCTAAGCGTGCGTTGGATCAAGCTTTGGAAAGCGATTCACGCGATTCATCTGCCAATATGATGATG
GGAGTGTTGTTGCAGCAAGAAGGTAGCAAGCTGAATCTAGAAAAAGCCGATGCCTATTTCAAGCGTGCCATTGCTGCTGA
CCCTCAAAATGCACAAGCGCGTAACAACTATGGTACGTATTTATATCAAGTAGAACGTTATAATGATGCGATTGAGCAGC
TCAGTATTGCTGGTGCAACACTGGGTTATGATCAGCGTTTTAGAGCATTAGAAAACGTAGGGCGTATTTATTTAAAGTTG
GGTGATGTCGCGAATGCCGAAAAATCATTCAAACAAGCCTTGCAAGCCAACCGCGATTCTTATGTTTCAATGTTAGAGTT
GTCTGAAATTTTATATTTAAGACAACAATTTGCACCCGCTCGACAATTGTATGAACAGTTTGTACGTGCGGTCGGGCAAA
AAAATCAGGGTGCGCGTGCCTTATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTA
GTGAACCAGCTGCGTGCATTATTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGT
ATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(92-134)

Tetratricopeptide repeat (TPR_16)

(46-80)

Tetratricopeptide repeat (TPR_16)

(154-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

73.978

100

0.74
  pilF Acinetobacter baylyi ADP1

73.228

94.424

0.691

Multiple sequence alignment