Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACNFWO_RS02035 Genome accession   NZ_CP181386
Coordinates   449228..450730 (-) Length   500 a.a.
NCBI ID   WP_132648432.1    Uniprot ID   A0A4R2M7B8
Organism   Rubrivivax gelatinosus isolate YF6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444228..455730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFWO_RS02010 - 444490..444978 (+) 489 WP_132648427.1 hypothetical protein -
  ACNFWO_RS02015 - 445028..445756 (+) 729 WP_132648428.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACNFWO_RS02020 - 445799..447301 (+) 1503 WP_132648429.1 amidase -
  ACNFWO_RS02025 - 447302..447940 (-) 639 WP_132648430.1 DsbA family oxidoreductase -
  ACNFWO_RS02030 - 447937..449172 (-) 1236 WP_132648431.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACNFWO_RS02035 rpoN/rpoN1 449228..450730 (-) 1503 WP_132648432.1 RNA polymerase factor sigma-54 Machinery gene
  ACNFWO_RS02040 lptB 450741..451499 (-) 759 WP_416461513.1 LPS export ABC transporter ATP-binding protein -
  ACNFWO_RS02045 lptA 451499..452119 (-) 621 WP_132648434.1 lipopolysaccharide transport periplasmic protein LptA -
  ACNFWO_RS02050 - 452235..452882 (-) 648 WP_132648435.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACNFWO_RS02055 - 452947..453564 (-) 618 WP_132648436.1 SPOR domain-containing protein -
  ACNFWO_RS02060 argS 453561..455261 (-) 1701 WP_132648437.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 55177.32 Da        Isoelectric Point: 4.6288

>NTDB_id=1099914 ACNFWO_RS02035 WP_132648432.1 449228..450730(-) (rpoN/rpoN1) [Rubrivivax gelatinosus isolate YF6]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEMDEESPTPFDAPAERAAVDRVAEREESDGAAE
AAPAEVDAAEFGTTERDDWENGTERDDFDGIRETPSSAAPESDDDLDPHERRAGTGTLQDHLRAQVAGMRLSPEDFAALN
VLIESLDDDGYLADPLEDIAARLADMLDLDDEEQREALADRLQCALRWLQSLEPTGVGARSLSECLVLQLRTLPRCEARE
VAIRICEHSLELLARRDVKKLTAATGAGEDLLRQAQALIVACEPKPGRPFSPAESNIIVPDVVVQKAGRGFKVLLNPDVM
PKLRINDLYAQAIRGQRGSAGGLSSRLQEARWFMKNIQQRFDTILRVSKAIVERQKGFFTHGAIAMKPLVLREIADELGL
HESTISRVTTAKYMATPQGTFELKYFFGSSLNTEAGGNASSTAVRALIQQLVSAEDSAKPLSDSQLSQMLEEQGIQVARR
TVAKYREALKIAPASLRRAI

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=1099914 ACNFWO_RS02035 WP_132648432.1 449228..450730(-) (rpoN/rpoN1) [Rubrivivax gelatinosus isolate YF6]
ATGAAGCCGTCCCTCCAAGTCCGCCTGTCGCAGCACCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTGGAGCTGCACCAGGAAGTCGAGCAGATGCTCGAGCAGAACCCGTTCCTGGAGATGGACGAGGAGTCAC
CGACGCCGTTCGACGCGCCGGCCGAACGCGCCGCCGTCGACCGTGTCGCCGAGCGCGAGGAGTCCGATGGCGCCGCCGAA
GCCGCCCCGGCCGAGGTCGACGCCGCCGAGTTCGGCACCACCGAACGCGACGACTGGGAAAACGGCACCGAGCGCGACGA
CTTCGACGGCATCCGCGAGACGCCGTCCTCGGCCGCGCCCGAAAGCGACGACGACCTCGACCCGCACGAGCGCCGCGCCG
GCACCGGCACGCTGCAGGACCACCTGCGCGCCCAGGTGGCGGGCATGCGCCTGTCGCCCGAGGACTTCGCCGCGCTGAAC
GTGCTGATCGAGTCGCTCGACGACGACGGCTACCTCGCCGACCCGCTGGAGGACATCGCCGCGCGCCTGGCCGACATGCT
GGACCTGGACGACGAGGAGCAGCGCGAGGCCCTGGCCGACCGCCTGCAGTGCGCCTTGCGCTGGCTGCAGAGCCTGGAGC
CCACCGGCGTCGGCGCGCGCTCGCTGTCCGAGTGTCTGGTGCTGCAGCTGCGCACGCTGCCGCGCTGCGAGGCACGCGAG
GTCGCGATCCGCATCTGCGAGCACTCTCTGGAGCTGCTGGCACGCCGCGACGTCAAGAAGCTCACCGCCGCCACCGGCGC
CGGCGAGGACCTGCTGCGCCAGGCGCAGGCGCTGATCGTCGCCTGCGAGCCCAAGCCGGGGCGGCCGTTCTCGCCGGCCG
AGTCCAACATCATCGTGCCCGACGTCGTCGTGCAGAAGGCGGGCCGCGGCTTCAAGGTGCTGCTGAACCCGGACGTGATG
CCCAAGCTGCGCATCAACGACCTGTACGCCCAGGCGATCCGCGGCCAGCGCGGCAGCGCCGGCGGGCTGTCCTCGCGGCT
GCAGGAGGCGCGCTGGTTCATGAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTATCCAAGGCCATCGTCGAGC
GCCAGAAGGGTTTCTTCACCCACGGCGCGATCGCGATGAAGCCGCTGGTGCTGCGCGAGATCGCTGACGAACTCGGCCTG
CACGAGAGCACGATCTCGCGCGTGACGACGGCCAAGTACATGGCCACGCCGCAGGGCACCTTCGAGCTGAAGTACTTCTT
CGGCAGCTCGCTGAACACCGAGGCCGGCGGCAACGCGTCGAGCACCGCGGTGCGCGCGCTGATCCAGCAGCTCGTCTCGG
CCGAGGACAGCGCCAAGCCGCTGTCGGACAGCCAGCTCTCGCAGATGCTCGAGGAGCAGGGCATCCAGGTCGCGCGCCGC
ACCGTCGCCAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCCTCGCTGCGCCGCGCGATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-498)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(131-329)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R2M7B8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

54.207

100

0.554

Multiple sequence alignment