Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACNANZ_RS20895 Genome accession   NZ_CP181370
Coordinates   4404880..4406433 (-) Length   517 a.a.
NCBI ID   WP_416400854.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain A12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4399880..4411433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNANZ_RS20870 (ACNANZ_20870) - 4399937..4400737 (+) 801 WP_416400849.1 hypothetical protein -
  ACNANZ_RS20875 (ACNANZ_20875) - 4400745..4401230 (-) 486 WP_416400850.1 putative toxin-antitoxin system toxin component, PIN family -
  ACNANZ_RS20880 (ACNANZ_20880) - 4401220..4402488 (-) 1269 WP_416400851.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACNANZ_RS20885 (ACNANZ_20885) - 4402601..4404424 (+) 1824 WP_416400852.1 methyl-accepting chemotaxis protein -
  ACNANZ_RS20890 (ACNANZ_20890) - 4404426..4404866 (-) 441 WP_416400853.1 acyl-CoA thioesterase -
  ACNANZ_RS20895 (ACNANZ_20895) rpoN/rpoN1 4404880..4406433 (-) 1554 WP_416400854.1 RNA polymerase factor sigma-54 Machinery gene
  ACNANZ_RS20900 (ACNANZ_20900) lptB 4406483..4407283 (-) 801 WP_094438705.1 LPS export ABC transporter ATP-binding protein -
  ACNANZ_RS20905 (ACNANZ_20905) lptA 4407280..4407924 (-) 645 WP_094438704.1 lipopolysaccharide transport periplasmic protein LptA -
  ACNANZ_RS20910 (ACNANZ_20910) - 4408044..4408700 (-) 657 WP_416400855.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACNANZ_RS20915 (ACNANZ_20915) - 4408923..4409543 (-) 621 WP_094438702.1 SPOR domain-containing protein -
  ACNANZ_RS20920 (ACNANZ_20920) argS 4409556..4411259 (-) 1704 WP_416400856.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 517 a.a.        Molecular weight: 56164.05 Da        Isoelectric Point: 4.5284

>NTDB_id=1099883 ACNANZ_RS20895 WP_416400854.1 4404880..4406433(-) (rpoN/rpoN1) [Alicycliphilus denitrificans strain A12]
MKPSLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTVDDAPPDEPLAPPAGEGGRQADDATSSGAA
DADWTGDEGQNDAQMPDIAAPTDWEGDTDIPGDDREWGSDAPAGAGSGSGDGDEADAADLAHGHESLADHLERQALALRL
SELDMAALRFLIGSLNDDGYLDEPLESLAASLADTDDPEQLDELVHRLTVALRLLQSLDPAGVGARGLAECLALQLQALD
REEEGDPEVIQAALCICRQPLDLLAKRDVRRLAQACAASDDLTRAAMALIARLEPRPGRRFADVERNVIVPDVIVRRTGR
GAQQSFSVRLNPDVMPRLRVHDVYANALRGGSKGDGHQALQARLQEARWFIKNIQQRFDTILRVSSAIVERQKNFFVHGE
LAMRPLVLREIADELGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRALIRQFVAAEDPHKPLSD
GQIADMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1554 bp        

>NTDB_id=1099883 ACNANZ_RS20895 WP_416400854.1 4404880..4406433(-) (rpoN/rpoN1) [Alicycliphilus denitrificans strain A12]
ATGAAGCCCAGCCTGTCGCTGCGCGTCTCGCAGCACCTGGCGCTCACGCCGCAGCTGCAGCAGTCCATCCGCCTGCTGCA
GCTGTCCACGCTGGAGCTGGCGCAGGAGGTCGAGCAGATGCTCGACGAGAACCCCTTCCTGGAGCGCACGGTGGACGACG
CGCCGCCGGACGAGCCGCTCGCGCCCCCCGCGGGCGAGGGCGGCCGCCAGGCCGACGATGCTACTTCTTCAGGAGCTGCC
GACGCAGACTGGACGGGCGATGAGGGCCAAAACGATGCCCAAATGCCCGATATCGCCGCGCCCACCGACTGGGAGGGCGA
CACCGACATCCCGGGCGACGACCGCGAGTGGGGCAGCGATGCGCCCGCGGGCGCCGGCAGCGGCTCCGGCGATGGCGACG
AGGCCGACGCGGCGGACCTGGCGCACGGCCACGAATCGCTCGCCGACCACCTTGAGCGCCAGGCCCTGGCGCTGCGCCTG
TCCGAGCTGGACATGGCGGCGCTGCGCTTCCTGATCGGCTCGCTCAACGACGACGGCTATCTGGACGAGCCGCTCGAATC
CCTGGCCGCGAGCCTGGCGGACACGGACGACCCCGAGCAGCTCGACGAACTGGTGCACCGCCTGACCGTGGCCCTGCGCC
TGCTGCAGAGCCTGGACCCCGCGGGCGTGGGCGCGCGCGGCCTGGCCGAATGCCTGGCGCTGCAGCTGCAGGCGCTGGAC
CGGGAGGAGGAGGGCGACCCCGAGGTGATCCAGGCCGCGCTGTGCATCTGCCGCCAGCCGCTCGACCTGCTGGCCAAGCG
CGACGTGCGCCGCCTGGCCCAGGCCTGCGCTGCGAGCGACGACCTCACGCGCGCCGCCATGGCGCTGATCGCGCGGCTGG
AGCCGCGCCCCGGGCGGCGCTTCGCCGACGTGGAGCGCAACGTCATCGTGCCCGACGTGATCGTCAGGAGGACCGGACGC
GGCGCGCAGCAGAGCTTCAGCGTCCGCCTCAACCCCGACGTGATGCCGCGCCTGCGCGTGCACGACGTCTACGCCAACGC
CCTGCGCGGCGGCAGCAAGGGCGACGGCCACCAGGCGCTGCAGGCCCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACA
TACAGCAGCGCTTCGACACCATCCTGCGCGTGTCCAGCGCCATCGTGGAGCGGCAGAAGAACTTCTTCGTGCATGGCGAG
CTGGCCATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGAGCTCGGCCTGCACGAATCCACCATCAGCCGCGTGACCAC
GGCCAAGTACATGGCCACGCCCCAGGGCACGTTCGAGCTCAAGTACTTCTTCGGCTCGGGCCTGGGCACCGAGACCGGCG
GCAGCGCGTCGAGCACGGCGGTGCGCGCGCTCATCCGGCAGTTCGTCGCCGCCGAGGACCCGCACAAGCCGCTGTCGGAC
GGCCAGATCGCCGACATGCTCAAGGAGCAGGGCATAGAGTGCGCGCGCCGCACCGTGGCCAAGTACCGCGAGGCGCTGAA
GATCGCGCCCGCCAACCTGCGCAAGGCGCTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-344)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(359-515)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.667

100

0.54

Multiple sequence alignment