Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACK8RZ_RS14110 Genome accession   NZ_CP181174
Coordinates   3096518..3097879 (-) Length   453 a.a.
NCBI ID   WP_003479244.1    Uniprot ID   A0A127ELS8
Organism   Clostridium perfringens strain XZ-E1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3091518..3102879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8RZ_RS14090 (ACK8RZ_14090) ispD 3092890..3093567 (-) 678 WP_416187734.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ACK8RZ_RS14095 (ACK8RZ_14095) - 3093581..3094687 (-) 1107 WP_003469613.1 PIN/TRAM domain-containing protein -
  ACK8RZ_RS14100 (ACK8RZ_14100) - 3094999..3095400 (+) 402 WP_416187735.1 DUF1573 domain-containing protein -
  ACK8RZ_RS14105 (ACK8RZ_14105) disA 3095434..3096498 (-) 1065 WP_003452233.1 DNA integrity scanning diadenylate cyclase DisA -
  ACK8RZ_RS14110 (ACK8RZ_14110) radA 3096518..3097879 (-) 1362 WP_003479244.1 DNA repair protein RadA Machinery gene
  ACK8RZ_RS14115 (ACK8RZ_14115) nagB 3098131..3098859 (-) 729 WP_003459367.1 glucosamine-6-phosphate deaminase -
  ACK8RZ_RS14120 (ACK8RZ_14120) - 3098856..3099578 (-) 723 WP_003459300.1 GntR family transcriptional regulator -
  ACK8RZ_RS14125 (ACK8RZ_14125) - 3099778..3099942 (+) 165 WP_003459343.1 hypothetical protein -
  ACK8RZ_RS14130 (ACK8RZ_14130) - 3100036..3100263 (+) 228 WP_003452255.1 glutaredoxin family protein -
  ACK8RZ_RS14135 (ACK8RZ_14135) - 3100386..3101834 (-) 1449 WP_416187736.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  ACK8RZ_RS14140 (ACK8RZ_14140) - 3101851..3102084 (-) 234 WP_003452243.1 glutaredoxin family protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49820.54 Da        Isoelectric Point: 6.5314

>NTDB_id=1099418 ACK8RZ_RS14110 WP_003479244.1 3096518..3097879(-) (radA) [Clostridium perfringens strain XZ-E1]
MAKVRSIYVCQNCGYETPKWMGKCPECNNWNTLVEEIRDTKSNQSSPKVERQIGELKKIKEIKSGEKERYDTGIGELNRV
LGGGLVKGSLTLISGDPGIGKSTLLLQTANNISKKYGKVLYVSGEESEEQIKIRGDRLKVDAEELYIVSETNLDVIEAYI
DKLEPAFIIIDSIQTIYRETVSSAPGSVSQVKECSNAVMRIAKGKNIPLFIVAHVTKQGDLAGPRVLEHMVDTVLSFEGE
RTEEFRILRTMKNRFGTTAEIGVFEMRGEGLMQVYDPSSMFLEDTSFNQEGSVVIGVMEGTRPILVEIQSLASETKAVMP
RRTSVGVENSRLSLILAVLEKKLRVPFYNTDVYVNVVGGLEIEGTTADLGIAISLVSSVKGKAASLEKLVVVGEVGLTGE
IRPISNCDRILNEAEKMGFLNAVVPYRSLEKLKGSKLNLIGVKTVREAIGKIF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1099418 ACK8RZ_RS14110 WP_003479244.1 3096518..3097879(-) (radA) [Clostridium perfringens strain XZ-E1]
GTGGCAAAGGTTAGAAGCATATATGTATGTCAAAATTGTGGATATGAAACACCTAAATGGATGGGTAAATGTCCAGAATG
TAATAATTGGAATACTTTAGTTGAAGAGATTAGAGATACCAAGAGTAATCAATCTTCTCCTAAGGTTGAAAGACAAATTG
GTGAGCTTAAAAAGATAAAAGAAATTAAATCAGGTGAAAAGGAAAGATATGATACTGGAATTGGTGAATTAAATAGAGTA
TTAGGTGGAGGGCTTGTTAAGGGGTCACTTACACTTATATCTGGTGACCCTGGAATAGGTAAATCTACACTTCTTTTACA
AACAGCCAATAATATTTCAAAAAAATATGGGAAAGTTTTATATGTTTCTGGGGAAGAATCAGAAGAACAAATAAAAATAA
GAGGAGACAGATTAAAAGTTGATGCAGAAGAACTTTATATAGTTTCCGAAACTAATCTTGATGTAATAGAAGCATATATA
GATAAACTTGAACCAGCTTTCATAATAATAGATTCAATACAAACAATTTATAGAGAAACAGTTTCTTCAGCACCAGGAAG
TGTTTCACAGGTTAAGGAATGCTCAAATGCTGTAATGAGAATTGCTAAAGGAAAAAATATTCCTTTATTTATAGTTGCTC
ACGTTACTAAGCAAGGGGATTTAGCAGGACCAAGAGTATTAGAACACATGGTGGATACTGTATTATCCTTTGAAGGGGAA
AGAACTGAAGAATTTAGAATATTAAGAACCATGAAAAATAGATTTGGTACTACGGCTGAAATAGGTGTTTTTGAAATGCG
TGGAGAGGGATTAATGCAGGTTTATGATCCATCTAGTATGTTTTTAGAGGACACTAGTTTTAATCAAGAAGGGTCAGTTG
TAATTGGAGTCATGGAAGGAACTAGACCTATTCTTGTGGAAATACAATCATTAGCATCTGAAACAAAAGCAGTTATGCCT
AGAAGAACCTCTGTAGGAGTAGAAAATTCAAGGTTAAGTTTAATATTAGCTGTATTAGAAAAAAAATTAAGAGTTCCTTT
TTATAATACTGATGTTTATGTAAATGTAGTAGGAGGACTTGAAATAGAAGGTACTACAGCAGACTTAGGAATTGCTATTT
CACTTGTATCCTCTGTTAAAGGTAAAGCAGCTAGCTTAGAAAAATTAGTTGTTGTAGGCGAGGTTGGGTTAACAGGTGAA
ATAAGACCAATATCAAATTGTGACAGAATATTAAATGAAGCAGAAAAAATGGGATTTTTAAATGCAGTTGTTCCTTATAG
AAGTTTAGAAAAATTAAAGGGAAGTAAATTAAACCTTATAGGAGTAAAAACTGTAAGGGAAGCAATAGGAAAGATATTTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-426)

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127ELS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.11

100

0.501
  radA Streptococcus pneumoniae Rx1

47.577

100

0.477
  radA Streptococcus pneumoniae D39

47.577

100

0.477
  radA Streptococcus pneumoniae R6

47.577

100

0.477
  radA Streptococcus pneumoniae TIGR4

47.577

100

0.477
  radA Streptococcus mitis SK321

47.357

100

0.475
  radA Streptococcus mitis NCTC 12261

47.357

100

0.475

Multiple sequence alignment