Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACM660_RS16635 Genome accession   NZ_CP181170
Coordinates   3528857..3529663 (-) Length   268 a.a.
NCBI ID   WP_412781845.1    Uniprot ID   -
Organism   Acinetobacter sp. SEK541     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3523857..3534663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM660_RS16615 (ACM660_16615) - 3524917..3525615 (-) 699 WP_004802650.1 YfgM family protein -
  ACM660_RS16620 (ACM660_16620) hisS 3525645..3526937 (-) 1293 WP_412781844.1 histidine--tRNA ligase -
  ACM660_RS16625 (ACM660_16625) ispG 3526934..3528049 (-) 1116 WP_004802647.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACM660_RS16630 (ACM660_16630) - 3528072..3528866 (-) 795 WP_004802646.1 helix-turn-helix domain-containing protein -
  ACM660_RS16635 (ACM660_16635) pilF 3528857..3529663 (-) 807 WP_412781845.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACM660_RS16640 (ACM660_16640) rlmN 3529681..3530916 (-) 1236 WP_412781846.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACM660_RS16645 (ACM660_16645) ndk 3531038..3531469 (-) 432 WP_004652531.1 nucleoside-diphosphate kinase -
  ACM660_RS16650 (ACM660_16650) iscX 3531577..3531777 (-) 201 WP_004652532.1 Fe-S cluster assembly protein IscX -
  ACM660_RS16655 (ACM660_16655) - 3531874..3532425 (-) 552 WP_005206348.1 phosphatase PAP2 family protein -
  ACM660_RS16660 (ACM660_16660) - 3532464..3533735 (-) 1272 WP_412781847.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 30456.39 Da        Isoelectric Point: 9.6938

>NTDB_id=1099391 ACM660_RS16635 WP_412781845.1 3528857..3529663(-) (pilF) [Acinetobacter sp. SEK541]
MNTTRPKTIIATAVVFTLLGLTACQSTSSTLKKDPEKAVQVRTQLAAEYIRNRDLDAAKRSLDQALKVDSRDANANMMMG
ILLQQEGSKTNKEKAEAYFKRAISSEPDNAQARNNYGTYLYQEQRYNDAIEQFTRAGATLGYEQRFQSLENLGRIYLKLG
DVANAEKSFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGLRIARANADQLGMQVLV
NQLRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 807 bp        

>NTDB_id=1099391 ACM660_RS16635 WP_412781845.1 3528857..3529663(-) (pilF) [Acinetobacter sp. SEK541]
TTGAATACCACTCGACCAAAAACGATTATTGCGACAGCTGTTGTGTTTACTTTACTTGGCTTAACAGCATGTCAATCAAC
ATCTTCAACATTAAAAAAAGATCCAGAAAAAGCAGTGCAAGTCCGGACCCAACTTGCAGCTGAATATATTCGCAATCGTG
ATTTAGACGCAGCTAAGCGTTCGCTGGACCAAGCCCTAAAGGTTGATTCACGTGATGCCAATGCCAATATGATGATGGGG
ATTTTGCTGCAACAAGAAGGTAGCAAGACCAATAAGGAAAAAGCAGAAGCTTATTTTAAGCGTGCAATTTCCTCAGAACC
TGATAATGCTCAAGCGCGTAATAATTACGGTACATATTTGTATCAAGAGCAACGCTATAATGATGCCATTGAGCAATTTA
CTCGTGCGGGTGCAACCTTAGGGTATGAACAGCGTTTTCAGTCGTTGGAAAACCTAGGACGAATCTATTTGAAATTGGGC
GATGTTGCGAATGCTGAAAAATCATTCAAACAAGCGCTACAAGCGAATCGAAATGCAACCATTTCAATGTTAGAGTTGGC
GGAAATTTTCTATTTGCAACAAAATAATAGAGATGCGTCGCAACTATACGAACAATATGTTCGTAACGTGGGACAGAAAA
ACCAAGGTGCTCGGGCACTTTGGATTGGTCTACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTGTTAGTC
AATCAGCTACGTGCGCTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATG
GAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(151-209)

Tetratricopeptide repeat (TPR_16)

(92-136)

Tetratricopeptide repeat (TPR_16)

(45-80)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.851

100

0.799
  pilF Acinetobacter baylyi ADP1

68.702

97.761

0.672

Multiple sequence alignment