Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   ACM6AD_RS00275 Genome accession   NZ_CP181138
Coordinates   59514..60863 (-) Length   449 a.a.
NCBI ID   WP_000815646.1    Uniprot ID   Q9XB96
Organism   Staphylococcus aureus strain AG21-0601     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39760..60863 59514..60863 within 0


Gene organization within MGE regions


Location: 39760..60863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM6AD_RS00180 (ACM6AD_00180) ccrB 39760..41388 (-) 1629 WP_001631035.1 cassette chromosome recombinase CcrB Machinery gene
  ACM6AD_RS00185 (ACM6AD_00185) ccrA 41385..42746 (-) 1362 WP_001631036.1 cassette chromosome recombinase CcrA Machinery gene
  ACM6AD_RS00190 (ACM6AD_00190) - 42933..43562 (-) 630 Protein_35 hypothetical protein -
  ACM6AD_RS00195 (ACM6AD_00195) - 43571..43909 (+) 339 Protein_36 recombinase family protein -
  ACM6AD_RS00200 (ACM6AD_00200) - 44021..44380 (-) 360 WP_001631039.1 hypothetical protein -
  ACM6AD_RS00205 (ACM6AD_00205) - 44587..44913 (+) 327 WP_000391495.1 ArsR/SmtB family transcription factor -
  ACM6AD_RS00210 (ACM6AD_00210) - 45243..47168 (+) 1926 WP_031783917.1 heavy metal translocating P-type ATPase -
  ACM6AD_RS00215 (ACM6AD_00215) - 48496..49752 (+) 1257 WP_000566670.1 MrcB family domain-containing protein -
  ACM6AD_RS00220 (ACM6AD_00220) - 50167..50406 (+) 240 WP_001282728.1 hypothetical protein -
  ACM6AD_RS00225 (ACM6AD_00225) - 50438..51112 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  ACM6AD_RS00230 (ACM6AD_00230) - 51326..52108 (-) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  ACM6AD_RS00235 (ACM6AD_00235) - 52259..52636 (-) 378 WP_000361059.1 DUF6262 family protein -
  ACM6AD_RS00240 (ACM6AD_00240) - 52643..54535 (-) 1893 WP_001557544.1 site-specific integrase -
  ACM6AD_RS00245 (ACM6AD_00245) - 54532..55617 (-) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  ACM6AD_RS00250 (ACM6AD_00250) - 55736..56053 (-) 318 WP_000659050.1 JAB domain-containing protein -
  ACM6AD_RS00255 (ACM6AD_00255) - 56074..56580 (-) 507 WP_001092169.1 DUF1643 domain-containing protein -
  ACM6AD_RS00260 (ACM6AD_00260) - 56598..56909 (-) 312 WP_000700853.1 DUF960 family protein -
  ACM6AD_RS00265 (ACM6AD_00265) - 56996..57346 (-) 351 WP_001631034.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ACM6AD_RS00270 (ACM6AD_00270) ccrB 57864..59492 (-) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  ACM6AD_RS00275 (ACM6AD_00275) ccrA 59514..60863 (-) 1350 WP_000815646.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52607.20 Da        Isoelectric Point: 9.9585

>NTDB_id=1098893 ACM6AD_RS00275 WP_000815646.1 59514..60863(-) (ccrA) [Staphylococcus aureus strain AG21-0601]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNTTLTNMTVRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CKDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1098893 ACM6AD_RS00275 WP_000815646.1 59514..60863(-) (ccrA) [Staphylococcus aureus strain AG21-0601]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATACCATGTTCATATCTTAAGCGTACACGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATACAACACTTACGAATATGACCGTTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCTCGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCAAAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9XB96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

100

100

1
  ccrA Staphylococcus aureus COL

74.833

100

0.748

Multiple sequence alignment