Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACM0O0_RS16295 Genome accession   NZ_CP180689
Coordinates   3477062..3477862 (-) Length   266 a.a.
NCBI ID   WP_213071882.1    Uniprot ID   -
Organism   Acinetobacter lactucae isolate nanjing     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3472062..3482862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0O0_RS16275 - 3473127..3473825 (-) 699 WP_368678082.1 YfgM family protein -
  ACM0O0_RS16280 hisS 3473854..3475146 (-) 1293 WP_062849551.1 histidine--tRNA ligase -
  ACM0O0_RS16285 ispG 3475143..3476258 (-) 1116 WP_016144301.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACM0O0_RS16290 - 3476280..3477071 (-) 792 WP_016142242.1 helix-turn-helix domain-containing protein -
  ACM0O0_RS16295 pilF 3477062..3477862 (-) 801 WP_213071882.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACM0O0_RS16300 rlmN 3477877..3479112 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACM0O0_RS16305 ndk 3479235..3479666 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  ACM0O0_RS16310 iscX 3479780..3479980 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  ACM0O0_RS16315 - 3480080..3480631 (-) 552 WP_016144298.1 phosphatase PAP2 family protein -
  ACM0O0_RS16320 - 3480656..3481942 (-) 1287 WP_274567684.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29997.17 Da        Isoelectric Point: 9.0424

>NTDB_id=1097472 ACM0O0_RS16295 WP_213071882.1 3477062..3477862(-) (pilF) [Acinetobacter lactucae isolate nanjing]
MSIPKLKTVLCMGIALAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKTNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMGRYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
VNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGVRVARANADKMGTQALVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1097472 ACM0O0_RS16295 WP_213071882.1 3477062..3477862(-) (pilF) [Acinetobacter lactucae isolate nanjing]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCACTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCGGAAAAAGCAGTAAAAGTGCGTACACAACTTGCTGCTGAACATATCCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACAGCAAACATGATGATGGGGATTTTG
CTACAGCAAGAAGGTAGCAAAACCAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGGGCGTTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GTGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGTGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAACGCAAGCATTAGTAAACCAA
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

92.105

100

0.921
  pilF Acinetobacter baylyi ADP1

75

97.744

0.733

Multiple sequence alignment