Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   ACK2OW_RS19170 Genome accession   NZ_CP180575
Coordinates   3675912..3676361 (+) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain K3C     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 3670912..3681361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS19150 (ACK2OW_19150) hxlR 3672124..3672486 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  ACK2OW_RS19155 (ACK2OW_19155) hxlA 3672718..3673350 (+) 633 WP_032724921.1 3-hexulose-6-phosphate synthase -
  ACK2OW_RS19160 (ACK2OW_19160) hxlB 3673356..3673913 (+) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  ACK2OW_RS19165 (ACK2OW_19165) tlpC 3674023..3675744 (+) 1722 WP_416042408.1 methyl-accepting chemotaxis protein TlpC -
  ACK2OW_RS19170 (ACK2OW_19170) nucA/comI 3675912..3676361 (+) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  ACK2OW_RS19175 (ACK2OW_19175) nin/comJ 3676388..3676786 (+) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  ACK2OW_RS19180 (ACK2OW_19180) bglC 3676823..3678256 (-) 1434 WP_172861418.1 6-phospho-beta-glucosidase -
  ACK2OW_RS19185 (ACK2OW_19185) yckD 3678410..3678742 (-) 333 WP_032724908.1 YckD family protein -
  ACK2OW_RS19190 (ACK2OW_19190) yckC 3678823..3679278 (-) 456 WP_064672016.1 RDD family protein -
  ACK2OW_RS19195 (ACK2OW_19195) - 3679298..3679495 (-) 198 WP_122060560.1 hypothetical protein -
  ACK2OW_RS19200 (ACK2OW_19200) yckB 3679657..3680520 (+) 864 WP_080481234.1 transporter substrate-binding domain-containing protein -
  ACK2OW_RS19205 (ACK2OW_19205) yckA 3680530..3681210 (+) 681 WP_003234624.1 amino acid ABC transporter permease -

Sequence


Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=1096811 ACK2OW_RS19170 WP_014475770.1 3675912..3676361(+) (nucA/comI) [Bacillus subtilis strain K3C]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1096811 ACK2OW_RS19170 WP_014475770.1 3675912..3676361(+) (nucA/comI) [Bacillus subtilis strain K3C]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987


Multiple sequence alignment