Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   ACK2OW_RS15800 Genome accession   NZ_CP180575
Coordinates   2942848..2943741 (+) Length   297 a.a.
NCBI ID   WP_015715882.1    Uniprot ID   A0A8E0SFH2
Organism   Bacillus subtilis strain K3C     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2937848..2948741
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS15780 (ACK2OW_15780) - 2938518..2940248 (+) 1731 WP_021479298.1 hypothetical protein -
  ACK2OW_RS15785 (ACK2OW_15785) ylqH 2940245..2940526 (+) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  ACK2OW_RS15790 (ACK2OW_15790) sucC 2940699..2941856 (+) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  ACK2OW_RS15795 (ACK2OW_15795) sucD 2941885..2942787 (+) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  ACK2OW_RS15800 (ACK2OW_15800) dprA 2942848..2943741 (+) 894 WP_015715882.1 DNA-processing protein DprA Machinery gene
  ACK2OW_RS15805 (ACK2OW_15805) topA 2943929..2946004 (+) 2076 WP_003245599.1 type I DNA topoisomerase -
  ACK2OW_RS15810 (ACK2OW_15810) trmFO 2946080..2947387 (+) 1308 WP_015715883.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  ACK2OW_RS15815 (ACK2OW_15815) xerC 2947455..2948369 (+) 915 WP_003231988.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32904.21 Da        Isoelectric Point: 9.9142

>NTDB_id=1096802 ACK2OW_RS15800 WP_015715882.1 2942848..2943741(+) (dprA) [Bacillus subtilis strain K3C]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQLYPKLQRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=1096802 ACK2OW_RS15800 WP_015715882.1 2942848..2943741(+) (dprA) [Bacillus subtilis strain K3C]
TTGGATCAGGCCGCTGCCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCTGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAACTTTATCCAAAGCTCCAGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCCGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAGCACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

98.99

100

0.99
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377

Multiple sequence alignment