Detailed information    

insolico Bioinformatically predicted

Overview

Name   rok   Type   Regulator
Locus tag   ACK2OW_RS14850 Genome accession   NZ_CP180575
Coordinates   2754042..2754626 (+) Length   194 a.a.
NCBI ID   WP_014113628.1    Uniprot ID   A0A0D1L215
Organism   Bacillus subtilis strain K3C     
Function   repression of comK (predicted from homology)   
Competence regulation

Genomic Context

Location: 2749042..2759626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS14820 (ACK2OW_14820) dapD 2749229..2749939 (+) 711 WP_003218680.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase -
  ACK2OW_RS14825 (ACK2OW_14825) dapI 2750009..2751133 (+) 1125 WP_106073886.1 N-acetyldiaminopimelate deacetylase -
  ACK2OW_RS14830 (ACK2OW_14830) ykuS 2751195..2751440 (+) 246 WP_003232382.1 YkuS family protein -
  ACK2OW_RS14835 (ACK2OW_14835) mscT 2751477..2752280 (-) 804 WP_003244994.1 small-conductance mechanosensitive channel protein MscT -
  ACK2OW_RS14840 (ACK2OW_14840) ahpA 2752517..2753059 (+) 543 WP_003245371.1 biofilm-specific peroxidase AhpA -
  ACK2OW_RS14845 (ACK2OW_14845) ykuV 2753130..2753576 (+) 447 WP_416042323.1 thiol-disulfide oxidoreductase YkuV -
  ACK2OW_RS14850 (ACK2OW_14850) rok 2754042..2754626 (+) 585 WP_014113628.1 transcriptional regulator Rok Regulator
  ACK2OW_RS14855 (ACK2OW_14855) cse15 2754667..2755632 (-) 966 WP_049139871.1 spore protein Cse15 -
  ACK2OW_RS14860 (ACK2OW_14860) mobA 2755769..2756368 (+) 600 WP_049139873.1 molybdenum cofactor guanylyltransferase -
  ACK2OW_RS14865 (ACK2OW_14865) moeB 2756419..2757438 (+) 1020 WP_049139875.1 molybdopterin-synthase adenylyltransferase MoeB -
  ACK2OW_RS14870 (ACK2OW_14870) glp 2757456..2758748 (+) 1293 WP_049139877.1 gephyrin-like molybdotransferase Glp -
  ACK2OW_RS14875 (ACK2OW_14875) mobB 2758709..2759230 (+) 522 WP_049139878.1 molybdopterin-guanine dinucleotide biosynthesis protein B -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22127.12 Da        Isoelectric Point: 9.7036

>NTDB_id=1096800 ACK2OW_RS14850 WP_014113628.1 2754042..2754626(+) (rok) [Bacillus subtilis strain K3C]
MFNEREALRLRLEQLNEAEVKVIREYQIERDKIYAKLRELDRNGSPSEIKKDFRSEKKPDSLPVLAELAAQEIRSYQPQS
QQQSVQPQLQSISSLPAGIPDGTTRRRRGTARPGSKAAKLREAAIKTLKRHNAAIKSSELQKEIEKESGLEIPNMTTFMQ
SLIKMYPEVKKPYRGQYILEGEIESAESAESANE

Nucleotide


Download         Length: 585 bp        

>NTDB_id=1096800 ACK2OW_RS14850 WP_014113628.1 2754042..2754626(+) (rok) [Bacillus subtilis strain K3C]
ATGTTTAATGAAAGAGAAGCTTTGCGCTTGCGGTTAGAACAATTAAATGAAGCTGAAGTGAAAGTCATTCGTGAATATCA
AATTGAACGTGATAAAATATACGCAAAATTAAGAGAGCTGGACAGAAATGGAAGCCCTTCCGAAATCAAAAAGGATTTCC
GTTCTGAAAAAAAACCTGACTCTCTGCCGGTTCTCGCTGAGCTTGCGGCTCAGGAAATCAGAAGCTATCAGCCGCAATCA
CAGCAGCAGTCTGTCCAGCCTCAGCTTCAATCTATTTCCTCTCTTCCTGCCGGTATACCAGACGGAACAACACGAAGAAG
AAGAGGAACGGCAAGACCGGGATCAAAAGCAGCGAAGCTGCGTGAAGCTGCTATTAAAACATTAAAACGCCACAACGCGG
CGATTAAGAGCTCCGAGCTTCAAAAAGAGATTGAAAAAGAAAGCGGTCTTGAAATCCCTAATATGACAACGTTTATGCAA
AGCTTAATTAAAATGTACCCGGAAGTGAAAAAACCATATCGCGGGCAGTACATTTTAGAGGGTGAAATCGAATCTGCAGA
ATCAGCTGAATCAGCTAATGAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L215

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rok Bacillus subtilis subsp. subtilis str. 168

100

97.423

0.974

Multiple sequence alignment