Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | ACK2OW_RS03895 | Genome accession | NZ_CP180575 |
| Coordinates | 663423..663833 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis strain K3C | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 658788..678377 | 663423..663833 | within | 0 |
Gene organization within MGE regions
Location: 658788..678377
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ACK2OW_RS03870 (ACK2OW_03870) | yqeF | 658955..659686 (-) | 732 | WP_029317882.1 | SGNH/GDSL hydrolase family protein | - |
| ACK2OW_RS03875 (ACK2OW_03875) | cwlH | 659938..660690 (-) | 753 | WP_416042547.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| ACK2OW_RS03880 (ACK2OW_03880) | yqeD | 660877..661503 (+) | 627 | WP_120028373.1 | TVP38/TMEM64 family protein | - |
| ACK2OW_RS03885 (ACK2OW_03885) | gnd | 661523..662416 (-) | 894 | WP_416042548.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| ACK2OW_RS03890 (ACK2OW_03890) | yqeB | 662668..663390 (+) | 723 | WP_049141193.1 | hypothetical protein | - |
| ACK2OW_RS03895 (ACK2OW_03895) | nucA/comI | 663423..663833 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| ACK2OW_RS03900 (ACK2OW_03900) | sigK | 664029..664757 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| ACK2OW_RS03905 (ACK2OW_03905) | - | 664757..664873 (+) | 117 | WP_141949575.1 | hypothetical protein | - |
| ACK2OW_RS03910 (ACK2OW_03910) | - | 664839..665036 (-) | 198 | Protein_777 | recombinase family protein | - |
| ACK2OW_RS03915 (ACK2OW_03915) | - | 664994..665172 (-) | 179 | Protein_778 | hypothetical protein | - |
| ACK2OW_RS03920 (ACK2OW_03920) | fumC | 665297..666685 (-) | 1389 | WP_032725151.1 | class II fumarate hydratase | - |
| ACK2OW_RS03925 (ACK2OW_03925) | - | 666852..667742 (+) | 891 | WP_077671576.1 | LysR family transcriptional regulator | - |
| ACK2OW_RS03930 (ACK2OW_03930) | - | 668684..669079 (+) | 396 | WP_046160622.1 | VOC family protein | - |
| ACK2OW_RS03935 (ACK2OW_03935) | - | 669819..670946 (-) | 1128 | WP_077671578.1 | Rap family tetratricopeptide repeat protein | - |
| ACK2OW_RS03940 (ACK2OW_03940) | phrE | 671356..671490 (+) | 135 | WP_032725165.1 | phosphatase RapE inhibitor PhrE | - |
| ACK2OW_RS03945 (ACK2OW_03945) | - | 671625..672476 (-) | 852 | WP_032725166.1 | aldo/keto reductase | - |
| ACK2OW_RS03950 (ACK2OW_03950) | - | 672646..673089 (+) | 444 | WP_029318107.1 | MerR family transcriptional regulator | - |
| ACK2OW_RS03955 (ACK2OW_03955) | - | 673287..673436 (-) | 150 | WP_234398249.1 | helix-turn-helix domain-containing protein | - |
| ACK2OW_RS03960 (ACK2OW_03960) | - | 674018..675943 (+) | 1926 | WP_416042549.1 | ribonuclease YeeF family protein | - |
| ACK2OW_RS03965 (ACK2OW_03965) | - | 675957..676244 (+) | 288 | WP_032726216.1 | hypothetical protein | - |
| ACK2OW_RS03970 (ACK2OW_03970) | - | 676548..676634 (+) | 87 | WP_072176383.1 | hypothetical protein | - |
| ACK2OW_RS03975 (ACK2OW_03975) | - | 676647..677087 (+) | 441 | WP_032726217.1 | SMI1/KNR4 family protein | - |
| ACK2OW_RS03980 (ACK2OW_03980) | - | 677186..677638 (+) | 453 | WP_416042550.1 | SMI1/KNR4 family protein | - |
| ACK2OW_RS03985 (ACK2OW_03985) | - | 677832..678377 (-) | 546 | WP_416042551.1 | SMI1/KNR4 family protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=1096755 ACK2OW_RS03895 WP_009967785.1 663423..663833(-) (nucA/comI) [Bacillus subtilis strain K3C]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=1096755 ACK2OW_RS03895 WP_009967785.1 663423..663833(-) (nucA/comI) [Bacillus subtilis strain K3C]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |