Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   ACK2OW_RS03645 Genome accession   NZ_CP180575
Coordinates   619980..620747 (-) Length   255 a.a.
NCBI ID   WP_049141200.1    Uniprot ID   -
Organism   Bacillus subtilis strain K3C     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 614980..625747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS03625 (ACK2OW_03625) ppsR 615154..615966 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  ACK2OW_RS03630 (ACK2OW_03630) ccpN 615992..616630 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  ACK2OW_RS03635 (ACK2OW_03635) glyS 616763..618802 (-) 2040 WP_032722129.1 glycine--tRNA ligase subunit beta -
  ACK2OW_RS03640 (ACK2OW_03640) glyQ 618795..619682 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  ACK2OW_RS03645 (ACK2OW_03645) recO 619980..620747 (-) 768 WP_049141200.1 DNA repair protein RecO Machinery gene
  ACK2OW_RS03650 (ACK2OW_03650) yqzL 620784..620927 (-) 144 WP_003230053.1 YqzL family protein -
  ACK2OW_RS03655 (ACK2OW_03655) era 621075..621980 (-) 906 WP_003230051.1 GTPase Era -
  ACK2OW_RS03660 (ACK2OW_03660) - 621961..622371 (-) 411 WP_003230049.1 cytidine deaminase -
  ACK2OW_RS03665 (ACK2OW_03665) unpK 622490..622861 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  ACK2OW_RS03670 (ACK2OW_03670) ybeY 622842..623315 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  ACK2OW_RS03675 (ACK2OW_03675) pgpH 623316..625451 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29326.05 Da        Isoelectric Point: 8.3080

>NTDB_id=1096751 ACK2OW_RS03645 WP_049141200.1 619980..620747(-) (recO) [Bacillus subtilis strain K3C]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGAFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=1096751 ACK2OW_RS03645 WP_049141200.1 619980..620747(-) (recO) [Bacillus subtilis strain K3C]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTGCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGATTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTTCATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment