Detailed information    

insolico Bioinformatically predicted

Overview


Name   recU   Type   Machinery gene
Locus tag   ACK2OW_RS02115 Genome accession   NZ_CP180575
Coordinates   350726..351346 (+) Length   206 a.a.
NCBI ID   WP_219144557.1    Uniprot ID   -
Organism   Bacillus subtilis strain K3C     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context


Location: 345726..356346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2OW_RS02075 (ACK2OW_02075) ptsA 346857..347363 (-) 507 WP_120028600.1 PTS glucose transporter subunit IIA -
  ACK2OW_RS02080 (ACK2OW_02080) ypqA 347501..347920 (+) 420 WP_072173284.1 Hsp20/alpha crystallin family protein -
  ACK2OW_RS02085 (ACK2OW_02085) sppG 347941..348318 (-) 378 WP_004398696.1 YppG family protein -
  ACK2OW_RS02090 (ACK2OW_02090) yppF 348506..348694 (+) 189 WP_003230690.1 YppF family protein -
  ACK2OW_RS02095 (ACK2OW_02095) yppE 348733..349104 (-) 372 WP_021481525.1 YppE family protein -
  ACK2OW_RS02100 (ACK2OW_02100) yppD 349150..349395 (-) 246 WP_021481526.1 DUF5446 family protein -
  ACK2OW_RS02105 (ACK2OW_02105) sspM 349594..349698 (+) 105 WP_003225606.1 acid-soluble spore protein SspM -
  ACK2OW_RS02110 (ACK2OW_02110) yppC 349723..350685 (-) 963 WP_411225449.1 DUF2515 domain-containing protein -
  ACK2OW_RS02115 (ACK2OW_02115) recU 350726..351346 (+) 621 WP_219144557.1 Holliday junction resolvase RecU Machinery gene
  ACK2OW_RS02120 (ACK2OW_02120) ponA 351368..354109 (+) 2742 WP_219144558.1 PBP1A family penicillin-binding protein -
  ACK2OW_RS02125 (ACK2OW_02125) ypoC 354185..354679 (-) 495 WP_219144559.1 YpoC family protein -
  ACK2OW_RS02130 (ACK2OW_02130) nth 354676..355335 (-) 660 WP_077671383.1 endonuclease III -
  ACK2OW_RS02135 (ACK2OW_02135) dnaD 355354..356052 (-) 699 WP_004398499.1 DNA replication protein DnaD -

Sequence


Protein


Download         Length: 206 a.a.        Molecular weight: 23987.14 Da        Isoelectric Point: 9.7222

>NTDB_id=1096739 ACK2OW_RS02115 WP_219144557.1 350726..351346(+) (recU) [Bacillus subtilis strain K3C]
MIRYPNGKTFQPKHSVSSQNSRKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIEHMKQVKAQDGICFVIISAFDQVYFLEADKLFYFWDRKE
KNGRKSIRKDELEETAYPISLGYAPRIDYISIIEQLYFSPSSGAKG

Nucleotide


Download         Length: 621 bp        

>NTDB_id=1096739 ACK2OW_RS02115 WP_219144557.1 350726..351346(+) (recU) [Bacillus subtilis strain K3C]
GTGATTCGGTATCCTAACGGAAAAACATTTCAGCCGAAACATTCGGTTTCATCCCAAAACAGTCGGAAAAGGGCCCCGTC
TTACAGTAATCGCGGAATGACCCTCGAAGATGACTTAAACGAAACGAATAAGTATTATCTGACAAACCAAATTGCCGTTA
TACACAAAAAGCCGACACCTGTTCAAATTGTAAATGTCCATTATCCAAAAAGAAGTGCCGCAGTGATTAAAGAAGCTTAC
TTTAAACAATCATCGACAACAGACTACAATGGGATTTACAAAGGGCGGTATATTGATTTTGAGGCGAAAGAAACGAAAAA
CAAGACCTCTTTCCCCCTGCAGAATTTTCATGACCATCAAATCGAGCATATGAAGCAGGTAAAGGCTCAAGACGGTATTT
GTTTTGTTATTATATCCGCTTTCGACCAGGTTTATTTTTTGGAAGCCGATAAGCTGTTTTATTTCTGGGACAGAAAAGAA
AAAAACGGCAGAAAATCAATTCGAAAAGATGAGTTGGAAGAAACAGCTTATCCGATTTCTCTTGGATACGCACCCAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCCATCATCTGGTGCGAAAGGTTGA

Domains


Predicted by InterproScan.

(31-193)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

99.515

100

0.995


Multiple sequence alignment