Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACMZ68_RS02450 Genome accession   NZ_CP180522
Coordinates   519541..520341 (+) Length   266 a.a.
NCBI ID   WP_415871686.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AP5091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514541..525341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMZ68_RS02425 (ACMZ68_02425) - 515457..516746 (+) 1290 WP_126645042.1 glycosyltransferase family 4 protein -
  ACMZ68_RS02430 (ACMZ68_02430) - 516771..517322 (+) 552 WP_415871685.1 phosphatase PAP2 family protein -
  ACMZ68_RS02435 (ACMZ68_02435) iscX 517422..517622 (+) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  ACMZ68_RS02440 (ACMZ68_02440) ndk 517736..518167 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  ACMZ68_RS02445 (ACMZ68_02445) rlmN 518291..519526 (+) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACMZ68_RS02450 (ACMZ68_02450) pilF 519541..520341 (+) 801 WP_415871686.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACMZ68_RS02455 (ACMZ68_02455) - 520332..521123 (+) 792 WP_032042051.1 helix-turn-helix domain-containing protein -
  ACMZ68_RS02460 (ACMZ68_02460) ispG 521145..522260 (+) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACMZ68_RS02465 (ACMZ68_02465) hisS 522257..523549 (+) 1293 WP_039764397.1 histidine--tRNA ligase -
  ACMZ68_RS02470 (ACMZ68_02470) - 523578..524276 (+) 699 WP_017398816.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30192.45 Da        Isoelectric Point: 9.0224

>NTDB_id=1096179 ACMZ68_RS02450 WP_415871686.1 519541..520341(+) (pilF) [Acinetobacter pittii strain AP5091]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISYEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
TNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1096179 ACMZ68_RS02450 WP_415871686.1 519541..520341(+) (pilF) [Acinetobacter pittii strain AP5091]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ATTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATATGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
ACGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(97-128)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.609

100

0.936
  pilF Acinetobacter baylyi ADP1

75.769

97.744

0.741

Multiple sequence alignment