Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACMX77_RS14060 Genome accession   NZ_CP180438
Coordinates   2670543..2670953 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain W-2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2665543..2675953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMX77_RS14030 (ACMX77_14030) - 2665630..2665770 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  ACMX77_RS14035 (ACMX77_14035) yqeF 2666076..2666807 (-) 732 WP_047182903.1 SGNH/GDSL hydrolase family protein -
  ACMX77_RS14040 (ACMX77_14040) cwlH 2667059..2667811 (-) 753 WP_047182904.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ACMX77_RS14045 (ACMX77_14045) yqeD 2667998..2668624 (+) 627 WP_047182905.1 TVP38/TMEM64 family protein -
  ACMX77_RS14050 (ACMX77_14050) gnd 2668643..2669536 (-) 894 WP_047182906.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ACMX77_RS14055 (ACMX77_14055) yqeB 2669788..2670510 (+) 723 WP_047182907.1 hypothetical protein -
  ACMX77_RS14060 (ACMX77_14060) nucA/comI 2670543..2670953 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ACMX77_RS14065 (ACMX77_14065) - 2671149..2671499 (+) 351 Protein_2724 sigma-70 family RNA polymerase sigma factor -
  ACMX77_RS14070 (ACMX77_14070) spoIVCA 2671527..2672987 (-) 1461 WP_253206441.1 site-specific DNA recombinase SpoIVCA -
  ACMX77_RS14075 (ACMX77_14075) - 2672945..2673123 (-) 179 Protein_2726 hypothetical protein -
  ACMX77_RS14080 (ACMX77_14080) mauJ 2673425..2674951 (+) 1527 WP_174616725.1 methylamine utilization protein MauJ -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=1095812 ACMX77_RS14060 WP_009967785.1 2670543..2670953(-) (nucA/comI) [Bacillus subtilis strain W-2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1095812 ACMX77_RS14060 WP_009967785.1 2670543..2670953(-) (nucA/comI) [Bacillus subtilis strain W-2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCTCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGTGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment